Literature DB >> 8317708

A continuous spectrophotometric assay for the aminoacylation of transfer RNA by alanyl-transfer RNA synthetase.

M X Wu1, K A Hill.   

Abstract

A continuous spectrophotometric assay is described for the aminoacylation reaction catalyzed by Escherichia coli alanyl-transfer RNA synthetase. The assay is based on coupling the alanyl-tRNA synthetase-dependent formation of AMP to the lactate dehydrogenase oxidation of NADH. Oxidation of NADH, as monitored at 340 nm, is shown to be stoichiometric with the formation of alanyl-tRNA(Ala). This assay will facilitate the rapid accumulation and analysis of kinetic data for alanyl-tRNA synthetase and should be applicable to aminoacyl-tRNA synthetases in general.

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Year:  1993        PMID： 8317708     DOI： 10.1006/abio.1993.1276

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  9 in total

1.  A universal plate format for increased throughput of assays that monitor multiple aminoacyl transfer RNA synthetase activities.

Authors:  Kirk Beebe; William Waas; Zhanna Druzina; Min Guo; Paul Schimmel
Journal:  Anal Biochem       Date:  2007-05-18       Impact factor: 3.365

2.  The Siderophore Synthetase IucA of the Aerobactin Biosynthetic Pathway Uses an Ordered Mechanism.

Authors:  Lisa S Mydy; Daniel C Bailey; Ketan D Patel; Matthew R Rice; Andrew M Gulick
Journal:  Biochemistry       Date:  2020-06-01       Impact factor: 3.162

3.  A broadly applicable continuous spectrophotometric assay for measuring aminoacyl-tRNA synthetase activity.

Authors:  A J Lloyd; H U Thomann; M Ibba; D Söll
Journal:  Nucleic Acids Res       Date:  1995-08-11       Impact factor: 16.971

4.  Structural and functional delineation of aerobactin biosynthesis in hypervirulent Klebsiella pneumoniae.

Authors:  Daniel C Bailey; Evan Alexander; Matthew R Rice; Eric J Drake; Lisa S Mydy; Courtney C Aldrich; Andrew M Gulick
Journal:  J Biol Chem       Date:  2018-04-04       Impact factor: 5.157

5.  A spectrophotometric assay for quantitative measurement of aminoacyl-tRNA synthetase activity.

Authors:  Igor Cestari; Kenneth Stuart
Journal:  J Biomol Screen       Date:  2012-11-07

6.  Structural basis for acyl acceptor specificity in the achromobactin biosynthetic enzyme AcsD.

Authors:  Stefan Schmelz; Catherine H Botting; Lijiang Song; Nadia F Kadi; Gregory L Challis; James H Naismith
Journal:  J Mol Biol       Date:  2011-08-01       Impact factor: 5.469

7.  Development of a High-Throughput Biochemical Assay to Screen for Inhibitors of Aerobactin Synthetase IucA.

Authors:  Daniel C Bailey; Brian P Buckley; Mikhail V Chernov; Andrew M Gulick
Journal:  SLAS Discov       Date:  2018-07-10       Impact factor: 3.341

8.  AcsD catalyzes enantioselective citrate desymmetrization in siderophore biosynthesis.

Authors:  Stefan Schmelz; Nadia Kadi; Stephen A McMahon; Lijiang Song; Daniel Oves-Costales; Muse Oke; Huanting Liu; Kenneth A Johnson; Lester G Carter; Catherine H Botting; Malcolm F White; Gregory L Challis; James H Naismith
Journal:  Nat Chem Biol       Date:  2009-02-01       Impact factor: 15.040

9.  Structural and Functional Characterization of Aerobactin Synthetase IucA from a Hypervirulent Pathotype of Klebsiella pneumoniae.

Authors:  Daniel C Bailey; Eric J Drake; Thomas D Grant; Andrew M Gulick
Journal:  Biochemistry       Date:  2016-06-16       Impact factor: 3.162
  9 in total

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