Literature DB >> 8315014

Proliferation of rat Pneumocystis carinii on cells sheeted on microcarrier beads in spinner flasks.

C H Lee1, N L Bauer, M M Shaw, M M Durkin, M S Bartlett, S F Queener, J W Smith.   

Abstract

A method of growing rat Pneumocystis carinii with human embryonic lung fibroblasts (HEL-299 cells) sheeted onto microcarrier beads has been developed. This method allows production of large quantities of P. carinii organisms with very little contamination of host cells. A fivefold increase in the numbers of organisms was achieved, as determined by organism count, antigen detection, and DNA quantification. The majority of organisms produced by this method are trophozoites.

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Year:  1993        PMID： 8315014      PMCID： PMC265602          DOI： 10.1128/jcm.31.6.1659-1662.1993

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  13 in total

1.  Isolation and characterization of rat lung Pneumocystis carinii gp120.

Authors:  J R Paulsrud; S F Queener; M S Bartlett; J W Smith
Journal:  J Protozool       Date:  1991 Nov-Dec

2.  Propagation of Pneumocystis carinii in Vero cell culture.

Authors:  L L Pifer; D Woods; W T Hughes
Journal:  Infect Immun       Date:  1978-04       Impact factor: 3.441

3.  Antimicrobial susceptibility of Pneumocystis carinii in culture.

Authors:  M S Bartlett; R Eichholtz; J W Smith
Journal:  Diagn Microbiol Infect Dis       Date:  1985-09       Impact factor: 2.803

4.  Propagation of Pneumocystis carinii in vitro.

Authors:  L L Pifer; W T Hughes; M J Murphy
Journal:  Pediatr Res       Date:  1977-04       Impact factor: 3.756

5.  Growth and metabolism of Pneumocystis carinii in axenic culture.

Authors:  M T Cushion; D Ebbets
Journal:  J Clin Microbiol       Date:  1990-06       Impact factor: 5.948

6.  Pneumocystis carinii: growth variables and estimates in the A549 and WI-38 VA13 human cell lines.

Authors:  M T Cushion; J J Ruffolo; M J Linke; P D Walzer
Journal:  Exp Parasitol       Date:  1985-08       Impact factor: 2.011

7.  Cultivation of Pneumocystis carinii with WI-38 cells.

Authors:  M S Bartlett; P A Verbanac; J W Smith
Journal:  J Clin Microbiol       Date:  1979-12       Impact factor: 5.948

8.  Growth and serial passage of Pneumocystis carinii in the A549 cell line.

Authors:  M T Cushion; P D Walzer
Journal:  Infect Immun       Date:  1984-05       Impact factor: 3.441

9.  The beta-tubulin gene from rat and human isolates of Pneumocystis carinii.

Authors:  T D Edlind; M S Bartlett; G A Weinberg; G N Prah; J W Smith
Journal:  Mol Microbiol       Date:  1992-11       Impact factor: 3.501

10.  Comparison of pulsed field gel electrophoresis karyotypes of Pneumocystis carinii derived from rat lung, cell culture, and ferret lung.

Authors:  G A Weinberg; M S Bartlett
Journal:  J Protozool       Date:  1991 Nov-Dec
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  15 in total

1.  Magnetic bead capture eliminates PCR inhibitors in samples collected from the airborne environment, permitting detection of Pneumocystis carinii DNA.

Authors:  N Maher; H K Dillon; S H Vermund; T R Unnasch
Journal:  Appl Environ Microbiol       Date:  2001-01       Impact factor: 4.792

2.  Gene arrays at Pneumocystis carinii telomeres.

Authors:  Scott P Keely; Hubert Renauld; Ann E Wakefield; Melanie T Cushion; A George Smulian; Nigel Fosker; Audrey Fraser; David Harris; Lee Murphy; Claire Price; Michael A Quail; Kathy Seeger; Sarah Sharp; Carolyn J Tindal; Tim Warren; Eduard Zuiderwijk; Barclay G Barrell; James R Stringer; Neil Hall
Journal:  Genetics       Date:  2005-06-18       Impact factor: 4.562

3.  Characterizations of neutral lipid fatty acids and cis-9,10-epoxy octadecanoic acid in Pneumocystis carinii carinii.

Authors:  E S Kaneshiro; J E Ellis; Z Guo; K Jayasimhulu; J N Maiorano; K A Kallam
Journal:  Infect Immun       Date:  1996-10       Impact factor: 3.441

4.  Inhibitors of delta24(25) sterol methyltransferase block sterol synthesis and cell proliferation in Pneumocystis carinii.

Authors:  J A Urbina; G Visbal; L M Contreras; G McLaughlin; R Docampo
Journal:  Antimicrob Agents Chemother       Date:  1997-07       Impact factor: 5.191

5.  Pneumocystis carinii pneumonia in scid mice induced by viable organisms propagated in vitro.

Authors:  J M Beck; R L Newbury; B E Palmer
Journal:  Infect Immun       Date:  1996-11       Impact factor: 3.441

6.  Continuous axenic cultivation of Pneumocystis carinii.

Authors:  S Merali; U Frevert; J H Williams; K Chin; R Bryan; A B Clarkson
Journal:  Proc Natl Acad Sci U S A       Date:  1999-03-02       Impact factor: 11.205

7.  Development and evaluation of a molecular viability assay for Pneumocystis carinii.

Authors:  N Maher; S Vermund; M Lasbury; C Lee; M Bartlett; T R Unnasch
Journal:  J Clin Microbiol       Date:  2000-05       Impact factor: 5.948

8.  Close association of Pneumocystis carinii from infected rat lung with culture cells as shown by light and electron microscopy.

Authors:  M S Bartlett; M P Goheen; C H Lee; M M Shaw; M M Durkin; J W Smith
Journal:  Parasitol Res       Date:  1994       Impact factor: 2.289

9.  Development of a rapid real-time PCR assay for quantitation of Pneumocystis carinii f. sp. carinii.

Authors:  Hans Henrik Larsen; Joseph A Kovacs; Frida Stock; Vibeke H Vestereng; Bettina Lundgren; Steven H Fischer; Vee J Gill
Journal:  J Clin Microbiol       Date:  2002-08       Impact factor: 5.948

10.  Semisynthetic echinocandins affect cell wall deposition of Pneumocystis carinii in vitro and in vivo.

Authors:  M S Bartlett; W L Current; M P Goheen; C J Boylan; C H Lee; M M Shaw; S F Queener; J W Smith
Journal:  Antimicrob Agents Chemother       Date:  1996-08       Impact factor: 5.191
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