Literature DB >> 11133478

Magnetic bead capture eliminates PCR inhibitors in samples collected from the airborne environment, permitting detection of Pneumocystis carinii DNA.

N Maher1, H K Dillon, S H Vermund, T R Unnasch.   

Abstract

PCR detection methods are useful in studies of organisms not amenable to culture. Inhibitors in environmental samples can interfere with such assays. We describe a magnetic bead DNA capture protocol that removes inhibitors from outdoor air samples, maintaining the sensitivity of a 16S Pneumocystis carinii mitochondrial rRNA gene-based PCR.

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Year:  2001        PMID： 11133478      PMCID： PMC92598          DOI： 10.1128/AEM.67.1.449-452.2001

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  24 in total

1.  Detection of Pneumocystis carinii DNA in air samples: likely environmental risk to susceptible persons.

Authors:  M S Bartlett; S H Vermund; R Jacobs; P J Durant; M M Shaw; J W Smith; X Tang; J J Lu; B Li; S Jin; C H Lee
Journal:  J Clin Microbiol       Date:  1997-10       Impact factor: 5.948

2.  Possible transfer of Pneumocystis carinii between immunodeficient patients.

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Journal:  Lancet       Date:  1990-09-08       Impact factor: 79.321

3.  Types of Pneumocystis carinii detected in air samples.

Authors:  M S Bartlett; J J Lu; C H Lee; P J Durant; S F Queener; J W Smith
Journal:  J Eukaryot Microbiol       Date:  1996 Sep-Oct       Impact factor: 3.346

4.  DNA sequences identical to Pneumocystis carinii f. sp. carinii and Pneumocystis carinii f. sp. hominis in samples of air spora.

Authors:  A E Wakefield
Journal:  J Clin Microbiol       Date:  1996-07       Impact factor: 5.948

5.  Sequences of Pneumocystis carinii f. sp. hominis strains associated with recurrent pneumonia vary at multiple loci.

Authors:  S P Keely; J R Stringer
Journal:  J Clin Microbiol       Date:  1997-11       Impact factor: 5.948

6.  Detection of Giardia in environmental waters by immuno-PCR amplification methods.

Authors:  M H Mahbubani; F W Schaefer; D D Jones; A K Bej
Journal:  Curr Microbiol       Date:  1998-02       Impact factor: 2.188

7.  Observations favouring Pneumocystis carinii pneumonia as a primary infection: a monoclonal antibody study on paraffin sections.

Authors:  P R Millard; A R Heryet
Journal:  J Pathol       Date:  1988-04       Impact factor: 7.996

8.  Identification of Pneumocystis carinii f. sp. hominis gene sequences in filtered air in hospital environments.

Authors:  M Olsson; C Lidman; S Latouche; A Björkman; P Roux; E Linder; M Wahlgren
Journal:  J Clin Microbiol       Date:  1998-06       Impact factor: 5.948

9.  Natural mode of acquisition for de novo infection with Pneumocystis carinii.

Authors:  W T Hughes
Journal:  J Infect Dis       Date:  1982-06       Impact factor: 5.226

10.  Nonspecific interstitial pneumonitis without evidence of Pneumocystis carinii in asymptomatic patients infected with human immunodeficiency virus (HIV).

Authors:  F P Ognibene; H Masur; P Rogers; W D Travis; A F Suffredini; I Feuerstein; V J Gill; B F Baird; J A Carrasquillo; J E Parrillo
Journal:  Ann Intern Med       Date:  1988-12-01       Impact factor: 25.391
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Journal:  Med Microbiol Immunol       Date:  2006-11-09       Impact factor: 3.402

Review 2.  Microbial Degradation of Forensic Samples of Biological Origin: Potential Threat to Human DNA Typing.

Authors:  Hirak Ranjan Dash; Surajit Das
Journal:  Mol Biotechnol       Date:  2018-02       Impact factor: 2.695

3.  Release of free DNA by membrane-impaired bacterial aerosols due to aerosolization and air sampling.

Authors:  Huajun Zhen; Taewon Han; Donna E Fennell; Gediminas Mainelis
Journal:  Appl Environ Microbiol       Date:  2013-10-04       Impact factor: 4.792

4.  Evaluation of PCR inhibitory effect of enrichment broths and comparison of DNA extraction methods for detection of Salmonella Enteritidis using real-time PCR assay.

Authors:  Ji Yeon Hyeon; In Gyun Hwang; Hyo Sun Kwak; Chankyu Park; In Soo Choi; Kun Ho Seo
Journal:  J Vet Sci       Date:  2010-06       Impact factor: 1.672

5.  Oligonucleotide based magnetic bead capture of Onchocerca volvulus DNA for PCR pool screening of vector black flies.

Authors:  Hemavathi Gopal; Hassan K Hassan; Mario A Rodríguez-Pérez; Laurent D Toé; Sara Lustigman; Thomas R Unnasch
Journal:  PLoS Negl Trop Dis       Date:  2012-06-19

6.  A comparison of methods used to unveil the genetic and metabolic pool in the built environment.

Authors:  Cinta Gomez-Silvan; Marcus H Y Leung; Katherine A Grue; Randeep Kaur; Xinzhao Tong; Patrick K H Lee; Gary L Andersen
Journal:  Microbiome       Date:  2018-04-16       Impact factor: 14.650

7.  Incorporating polymerase chain reaction-based identification, population characterization, and quantification of microorganisms into aerosol science: A review.

Authors:  Jordan Peccia; Mark Hernandez
Journal:  Atmos Environ (1994)       Date:  2006-04-18       Impact factor: 4.798

8.  Ultrasensitive hybridization capture: Reliable detection of <1 copy/mL short cell-free DNA from large-volume urine samples.

Authors:  Amy Oreskovic; Barry R Lutz
Journal:  PLoS One       Date:  2021-02-26       Impact factor: 3.240

9.  Pneumocystis murina colonization in immunocompetent surfactant protein A deficient mice following environmental exposure.

Authors:  Michael J Linke; Alan D Ashbaugh; Jeffery A Demland; Peter D Walzer
Journal:  Respir Res       Date:  2009-02-19
  9 in total

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