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Literature DB >> 8263195

Detection of caprine arthritis-encephalitis virus by polymerase chain reaction.

Abstract

Detection of caprine arthritis-encephalitis virus (CAEV) infection in goats is currently limited to serologic testing or cell culture. We developed a polymerase chain reaction (PCR) assay to detect CAEV sequences in peripheral blood mononuclear cells (PBMC), synovial fluid cells (SFC), and milk cells (MC) obtained from infected goats. Results were positive for 18 of 20 PBMC, 8 of 8 MC, and 5 of 5 SFC samples from seropositive goats, whereas 3 of 33 PBMC samples and none of 8 MC or 5 SFC samples from seronegative goats were positive. Two of the PCR-positive and seronegative goats seroconverted upon follow-up testing 2 months later. This PCR assay provides a useful method for detecting CAEV infection in goats.

Entities: Disease Species

Mesh：

Year: 1993 PMID： 8263195 PMCID： PMC266206 DOI： 10.1128/jcm.31.11.3042-3043.1993

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

13 in total

Review 1. Caprine arthritis encephalitis lentivirus transmission and disease.

Authors: T C McGuire; K I O'Rourke; D P Knowles; W P Cheevers
Journal: Curr Top Microbiol Immunol Date: 1990 Impact factor: 4.291

2. Avoiding false positives with PCR.

Authors: S Kwok; R Higuchi
Journal: Nature Date: 1989-05-18 Impact factor: 49.962

3. Infectious leukoencephalomyelitis of young goats.

Authors: L C Cork; W J Hadlow; T B Crawford; J R Gorham; R C Piper
Journal: J Infect Dis Date: 1974-02 Impact factor: 5.226

4. Nucleotide sequence and transcriptional analysis of molecular clones of CAEV which generate infectious virus.

Authors: M Saltarelli; G Querat; D A Konings; R Vigne; J E Clements
Journal: Virology Date: 1990-11 Impact factor: 3.616

5. Caprine arthritis-encephalitis: clinical features and presence of antibody in selected goat populations.

Authors: T B Crawford; D S Adams
Journal: J Am Vet Med Assoc Date: 1981-04-01 Impact factor: 1.936

6. Biological characterization of the virus causing leukoencephalitis and arthritis in goats.

Authors: O Narayan; J E Clements; J D Strandberg; L C Cork; D E Griffin
Journal: J Gen Virol Date: 1980-09 Impact factor: 3.891

7. Biochemical characterization of the virus causing leukoencephalitis and arthritis in goats.

Authors: J E Clements; O Narayan; L C Cork
Journal: J Gen Virol Date: 1980-10 Impact factor: 3.891

8. Ovine lentivirus is macrophagetropic and does not replicate productively in T lymphocytes.

Authors: M D Gorrell; M R Brandon; D Sheffer; R J Adams; O Narayan
Journal: J Virol Date: 1992-05 Impact factor: 5.103

9. Chronic arthritis in goats caused by a retrovirus.

Authors: T B Crawford; D S Adams; W P Cheevers; L C Cork
Journal: Science Date: 1980-02-29 Impact factor: 47.728

10. The pathogenesis of viral leukoencephalomyelitis-arthritis of goats. I. Persistent viral infection with progressive pathologic changes.

Authors: L C Cork; O Narayan
Journal: Lab Invest Date: 1980-06 Impact factor: 5.662

10 in total

10. (Non-)Sense of Milk Testing in Small Ruminant Lentivirus Control Programs in Goats. Comparative Analysis of Antibody Detection and Molecular Diagnosis in Blood and Milk.

Authors: Nadjah Radia Adjadj; Jo Vicca; Rodolphe Michiels; Nick De Regge
Journal: Viruses Date: 2019-12-18 Impact factor: 5.048

10 in total

Detection of caprine arthritis-encephalitis virus by polymerase chain reaction.

Review 1. Caprine arthritis encephalitis lentivirus transmission and disease.

2. Avoiding false positives with PCR.

3. Infectious leukoencephalomyelitis of young goats.

4. Nucleotide sequence and transcriptional analysis of molecular clones of CAEV which generate infectious virus.

5. Caprine arthritis-encephalitis: clinical features and presence of antibody in selected goat populations.

6. Biological characterization of the virus causing leukoencephalitis and arthritis in goats.

7. Biochemical characterization of the virus causing leukoencephalitis and arthritis in goats.

8. Ovine lentivirus is macrophagetropic and does not replicate productively in T lymphocytes.

9. Chronic arthritis in goats caused by a retrovirus.

10. The pathogenesis of viral leukoencephalomyelitis-arthritis of goats. I. Persistent viral infection with progressive pathologic changes.

1. Rapid diagnosis of Argentine hemorrhagic fever by reverse transcriptase PCR-based assay.

2. PCR detection of lentiviral GAG segment DNA in the white blood cells of sheep and goats.

3. Maedi in slaughtered sheep: a pathology and polymerase chain reaction study in southwestern Iran.

4. Pathogenic mechanisms of caprine arthritis-encephalitis virus.

5. Identification of the ovine mannose receptor and its possible role in Visna/Maedi virus infection.

Review 6. Advances in diagnosis of respiratory diseases of small ruminants.

7. Development and Field Testing of a Real-Time PCR Assay for Caprine Arthritis-Encephalitis-Virus (CAEV).

Review 8. Small ruminant lentiviruses: genetic variability, tropism and diagnosis.

Review 9. Applications of DNA amplification techniques in veterinary diagnostics.

10. (Non-)Sense of Milk Testing in Small Ruminant Lentivirus Control Programs in Goats. Comparative Analysis of Antibody Detection and Molecular Diagnosis in Blood and Milk.