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Literature DB >> 825858

Expression of the thymidylate synthetase gene of the Bacillus subtilis bacteriophage Phi-3-T in Escherichia coli.

S D Ehrlich, H Bursztyn-Pettegrew, I Stroynowski, J Lederberg.

Abstract

The thymidylate synthetase gene of B. subtilis bacteriophage Phi-3-T, when cloned in plasmids pSC101 or pMB9 is expressed in E. coli. The promoter of the cloned gene is likely to originate in Phi-3-T. Rearrangements of hybrid plasmid sequences during the cloning have been noted. B. subtilis strains can be transformed with hybrid DNAs. The transformants contain sequences of Phi-3-T, but not those of plasmid vectors.

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Year: 1976 PMID： 825858 PMCID： PMC431360 DOI： 10.1073/pnas.73.11.4145

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

14 in total

1. Site specific recA--independent recombination between bacterial plasmids: involvement of palindromes at the recombinational loci.

Authors: D J Kopecko; S N Cohen
Journal: Proc Natl Acad Sci U S A Date: 1975-04 Impact factor: 11.205

2. Transposition of ampicillin resistance from RP4 to other replicons.

Authors: R W Hedges; A E Jacob
Journal: Mol Gen Genet Date: 1974

3. Construction of biologically functional bacterial plasmids in vitro.

Authors: S N Cohen; A C Chang; H W Boyer; R B Helling
Journal: Proc Natl Acad Sci U S A Date: 1973-11 Impact factor: 11.205

4. Acquisition of thymidylate synthetase activity by a thymine-requiring mutant of Bacillus subtilis following infection by the temperate phage phi 3.

Authors: R G Tucker
Journal: J Gen Virol Date: 1969-06 Impact factor: 3.891

5. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors: D B Clewell; D R Helinski
Journal: Proc Natl Acad Sci U S A Date: 1969-04 Impact factor: 11.205

6. Electrophoretic separation of Bacillus subtilis genes.

Authors: R M Harris-Warrick; Y Elkana; S D Ehrlich; J Lederberg
Journal: Proc Natl Acad Sci U S A Date: 1975-06 Impact factor: 11.205

7. Characterization of temperate bacteriophages of Bacillus subtilis by the restriction endonuclease EcoRI: evidence for three different temperate bacteriophages.

Authors: G A Wilson; M T Williams; H W Baney; F E Young
Journal: J Virol Date: 1974-10 Impact factor: 5.103

8. Expression of Actinomyces viscosus antigens in Escherichia coli: cloning of a structural gene (fimA) for type 2 fimbriae.

Authors: J A Donkersloot; J O Cisar; M E Wax; R J Harr; B M Chassy
Journal: J Bacteriol Date: 1985-06 Impact factor: 3.490

9. Transformation of Escherichia coli and Bacillus subtilis with a hybrid plasmid molecule.

Authors: I Mahler; H O Halvorson
Journal: J Bacteriol Date: 1977-07 Impact factor: 3.490

10. Replication and expression of plasmids from Staphylococcus aureus in Bacillus subtilis.

Authors: S D Ehrlich
Journal: Proc Natl Acad Sci U S A Date: 1977-04 Impact factor: 11.205

Expression of the thymidylate synthetase gene of the Bacillus subtilis bacteriophage Phi-3-T in Escherichia coli.

1. Site specific recA--independent recombination between bacterial plasmids: involvement of palindromes at the recombinational loci.

2. Transposition of ampicillin resistance from RP4 to other replicons.

3. Construction of biologically functional bacterial plasmids in vitro.

4. Acquisition of thymidylate synthetase activity by a thymine-requiring mutant of Bacillus subtilis following infection by the temperate phage phi 3.

5. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

6. Electrophoretic separation of Bacillus subtilis genes.

7. Characterization of temperate bacteriophages of Bacillus subtilis by the restriction endonuclease EcoRI: evidence for three different temperate bacteriophages.

8. A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster.

9. Restriction and modification in B. subtilis. Biological aspects.

10. Physical heterogeneity among Bacillus subtilis deoxyribonucleic acid molecules carrying particular genetic markers.

1. Cloning of Clostridium acetobutylicum genes and their expression in Escherichia coli and Bacillus subtilis.

2. Recombinant plasmids capable to replication in B. subtilis and E. coli.

3. DNA cloning in Bacillus subtilis.

4. Bacteriophage conversion of spore-negative mutants to spore-positive in Bacillus pumilus.

5. Organization of the pE194 genome.

6. Ribosomal RNA precursors of Bacillus subtilis.

7. New cryptic plasmid of Bacillus subtilis and restriction analysis of other plasmids found by general screening.

8. Expression of Actinomyces viscosus antigens in Escherichia coli: cloning of a structural gene (fimA) for type 2 fimbriae.

9. Transformation of Escherichia coli and Bacillus subtilis with a hybrid plasmid molecule.

10. Replication and expression of plasmids from Staphylococcus aureus in Bacillus subtilis.