Literature DB >> 2860095

Expression of Actinomyces viscosus antigens in Escherichia coli: cloning of a structural gene (fimA) for type 2 fimbriae.

J A Donkersloot, J O Cisar, M E Wax, R J Harr, B M Chassy.   

Abstract

A cosmid gene library of Actinomyces viscosus T14V was prepared in Escherichia coli to examine the expression of A. viscosus antigens and to gain insight into the structure of A. viscosus type 1 and type 2 fimbriae. Out of this library of 550 clones, 28 reacted in a colony immunoassay with antibodies against A. viscosus cells. The proteins responsible for these reactions were identified in three clones. Clones AV1209 and AV2009 displayed nonfimbrial antigens with subunits of 40 and 58 kilodaltons, respectively. Clone AV1402 showed a 59-kilodalton protein that reacted with monospecific antibody against type 2 fimbriae and that comigrated with a subunit of type 2 fimbriae during sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This indicates that AV1402 expresses a gene (fimA) for a subunit of A. viscosus type 2 fimbriae.

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Year:  1985        PMID： 2860095      PMCID： PMC215885          DOI： 10.1128/jb.162.3.1075-1078.1985

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  25 in total

1.  A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.

Authors:  L Clarke; J Carbon
Journal:  Cell       Date:  1976-09       Impact factor: 41.582

2.  A small cosmid for efficient cloning of large DNA fragments.

Authors:  B Hohn; J Collins
Journal:  Gene       Date:  1980-11       Impact factor: 3.688

3.  A restriction map of the bacteriophage T4 genome.

Authors:  P H O'Farrell; E Kutter; M Nakanishi
Journal:  Mol Gen Genet       Date:  1980

4.  Method for the lysis of Gram-positive, asporogenous bacteria with lysozyme.

Authors:  B M Chassy; A Giuffrida
Journal:  Appl Environ Microbiol       Date:  1980-01       Impact factor: 4.792

5.  Organization of transcriptional signals in plasmids pBR322 and pACYC184.

Authors:  D Stüber; H Bujard
Journal:  Proc Natl Acad Sci U S A       Date:  1981-01       Impact factor: 11.205

6.  Fibril-mediated adherence of Actinomyces viscosus to saliva-treated hydroxyapatite.

Authors:  T T Wheeler; W B Clark
Journal:  Infect Immun       Date:  1980-05       Impact factor: 3.441

7.  Antibodies against the Ag2 fimbriae of Actinomyces viscosus T14V inhibit lactose-sensitive bacterial adherence.

Authors:  G J Revis; A E Vatter; A J Crowle; J O Cisar
Journal:  Infect Immun       Date:  1982-06       Impact factor: 3.441

8.  Detection and localization of a lectin on Actinomyces viscosus T14V by monoclonal antibodies.

Authors:  J O Cisar; E L Barsumian; S H Curl; A E Vatter; A L Sandberg; R P Siraganian
Journal:  J Immunol       Date:  1981-10       Impact factor: 5.422

9.  Expression of the thymidylate synthetase gene of the Bacillus subtilis bacteriophage Phi-3-T in Escherichia coli.

Authors:  S D Ehrlich; H Bursztyn-Pettegrew; I Stroynowski; J Lederberg
Journal:  Proc Natl Acad Sci U S A       Date:  1976-11       Impact factor: 11.205

10.  Molecular cloning of the gene for the beta-lactamase of Bacillus licheniformis and its expression in Escherichia coli.

Authors:  W J Brammar; S Muir; A McMorris
Journal:  Mol Gen Genet       Date:  1980-04
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  21 in total

Review 1.  Sortases and the art of anchoring proteins to the envelopes of gram-positive bacteria.

Authors:  Luciano A Marraffini; Andrea C Dedent; Olaf Schneewind
Journal:  Microbiol Mol Biol Rev       Date:  2006-03       Impact factor: 11.056

2.  Sortase-catalyzed assembly of distinct heteromeric fimbriae in Actinomyces naeslundii.

Authors:  Arunima Mishra; Asis Das; John O Cisar; Hung Ton-That
Journal:  J Bacteriol       Date:  2007-02-02       Impact factor: 3.490

3.  Mutants of Actinomyces viscosus T14V lacking type 1, type 2, or both types of fimbriae.

Authors:  J O Cisar; A E Vatter; W B Clark; S H Curl; S Hurst-Calderone; A L Sandberg
Journal:  Infect Immun       Date:  1988-11       Impact factor: 3.441

4.  Cloning and nucleotide sequence of a gene for Actinomyces naeslundii WVU45 type 2 fimbriae.

Authors:  M K Yeung; J O Cisar
Journal:  J Bacteriol       Date:  1988-09       Impact factor: 3.490

5.  Use of lytic bacteriophage for Actinomyces viscosus T14V as a probe for cell surface components mediating intergeneric coaggregation.

Authors:  A L Delisle; J A Donkersloot; P E Kolenbrander; C A Tylenda
Journal:  Infect Immun       Date:  1988-01       Impact factor: 3.441

6.  Synthesis and function of Actinomyces naeslundii T14V type 1 fimbriae require the expression of additional fimbria-associated genes.

Authors:  M K Yeung; P A Ragsdale
Journal:  Infect Immun       Date:  1997-07       Impact factor: 3.441

7.  Actinomyces naeslundii displays variant fimP and fimA fimbrial subunit genes corresponding to different types of acidic proline-rich protein and beta-linked galactosamine binding specificity.

Authors:  K Hallberg; C Holm; U Ohman; N Strömberg
Journal:  Infect Immun       Date:  1998-09       Impact factor: 3.441

8.  Identification and preliminary characterization of a Streptococcus sanguis fibrillar glycoprotein.

Authors:  E J Morris; N Ganeshkumar; M Song; B C McBride
Journal:  J Bacteriol       Date:  1987-01       Impact factor: 3.490

9.  Cloning and expression of a type 1 fimbrial subunit of Actinomyces viscosus T14V.

Authors:  M K Yeung; B M Chassy; J O Cisar
Journal:  J Bacteriol       Date:  1987-04       Impact factor: 3.490

10.  Cloning of the Streptococcus gordonii PK488 gene, encoding an adhesin which mediates coaggregation with Actinomyces naeslundii PK606.

Authors:  R N Andersen; N Ganeshkumar; P E Kolenbrander
Journal:  Infect Immun       Date:  1993-03       Impact factor: 3.441
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