Literature DB >> 8253988

Evaluation of a genetically engineered cell line and a histochemical beta-galactosidase assay to detect herpes simplex virus in clinical specimens.

E C Stabell1, S R O'Rourke, G A Storch, P D Olivo.   

Abstract

A novel histochemical method was compared with a cytopathic effect (CPE) assay for the identification of herpes simplex virus (HSV) in clinical specimens. The method utilizes a stably transformed cell line (BHKICP6LacZ-5) that expresses beta-galactosidase only after infection with HSV. A total of 96 specimens submitted to our diagnostic virology laboratory were analyzed. Thirty-one specimens contained HSV as evidenced by positive CPE, and all were positive for beta-galactosidase staining. CPE were not evident for 2 or more days in 15 of the 31 positive specimens, whereas the histochemical stain was positive in all 31 positives by 16 to 24 h. This preliminary study shows that the BHKICP6LacZ-5 cell line can be used in a rapid, sensitive, and specific assay for the detection of HSV in clinical specimens.

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Year:  1993        PMID： 8253988      PMCID： PMC266019          DOI： 10.1128/jcm.31.10.2796-2798.1993

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  6 in total

1.  Comparison of enzyme immunoassay, shell vial culture, and conventional cell culture for the rapid detection of herpes simplex virus.

Authors:  S L Johnston; C S Siegel
Journal:  Diagn Microbiol Infect Dis       Date:  1990 May-Jun       Impact factor: 2.803

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Authors:  D J Goldstein; S K Weller
Journal:  J Virol       Date:  1988-01       Impact factor: 5.103

3.  Detection and serotyping of herpes simplex virus in MRC-5 cells by use of centrifugation and monoclonal antibodies 16 h postinoculation.

Authors:  C A Gleaves; D J Wilson; A D Wold; T F Smith
Journal:  J Clin Microbiol       Date:  1985-01       Impact factor: 5.948

4.  Comparison of primary rabbit kidney and MRC-5 cells and two stain procedures for herpes simplex virus detection by a shell vial centrifugation method.

Authors:  E M Peterson; B L Hughes; S L Aarnaes; L M de la Maza
Journal:  J Clin Microbiol       Date:  1988-02       Impact factor: 5.948

5.  Impact of cell culture sensitivity and virus concentration on rapid detection of herpes simplex virus by cytopathic effects and immunoperoxidase staining.

Authors:  L S Zhao; M L Landry; E S Balkovic; G D Hsiung
Journal:  J Clin Microbiol       Date:  1987-08       Impact factor: 5.948

6.  Isolation of a cell line for rapid and sensitive histochemical assay for the detection of herpes simplex virus.

Authors:  E C Stabell; P D Olivo
Journal:  J Virol Methods       Date:  1992-08       Impact factor: 2.014
  6 in total
  14 in total

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Review 7.  Virological and Immunological Outcomes of Coinfections.

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8.  A rapid and simple isothermal nucleic acid amplification test for detection of herpes simplex virus types 1 and 2.

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9.  Multicenter Evaluation of Meridian Bioscience HSV 1&2 Molecular Assay for Detection of Herpes Simplex Virus 1 and 2 from Clinical Cutaneous and Mucocutaneous Specimens.

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Review 10.  Light microscopy, culture, molecular, and serologic methods for detection of herpes simplex virus.

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