Literature DB >> 825322

[The chromophore of blue dextran: a powerful ligand for the purification of interferon by affinity chromatography].

J de Maeyer-Guignard, E de Maeyer.   

Abstract

The chromophore of blue dextran can be used as ligand for affinity chromatography of interferon. One passage of a crude interferon preparation on a blue-dextran-sepharose column eliminates over 90% of the contaminating proteins. Desorption with an aqueous buffer of high molarity yields interferon preparations with specific activities of up to 4.5 X 10(6) units per milligram of protein and the recovery is 100%.

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Year:  1976        PMID: 825322

Source DB:  PubMed          Journal:  C R Acad Hebd Seances Acad Sci D


  3 in total

1.  Biological properties of mouse interferon bound to a high molecular weight carrier: Blue Dextran 2000.

Authors:  A D Inglot; B Kisielow; O Inglot
Journal:  Arch Virol       Date:  1979       Impact factor: 2.574

2.  The effect of matrix on the binding of albumin to immobilized Cibacron Blue.

Authors:  S Angal; P D Dean
Journal:  Biochem J       Date:  1977-10-01       Impact factor: 3.857

3.  Binding of mouse interferon to polynucleotides.

Authors:  J De Maeyer-Guignard; M N Thang; E De Maeyer
Journal:  Proc Natl Acad Sci U S A       Date:  1977-09       Impact factor: 11.205

  3 in total

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