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Literature DB >> 588263

The effect of matrix on the binding of albumin to immobilized Cibacron Blue.

Abstract

With Cibacron Blue as a ligand, the suitability of Sepharose, Sephacryl, cellulose and Ultrogel as column supports for the affinity chromatography of human serum albumin was examined. Sepharose and Sephacryl were the most effective matrices. Albumin was readily removed from such columns by using an electrophoretic desorption technique.

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Year: 1977 PMID： 588263 PMCID： PMC1183653 DOI： 10.1042/bj1670301

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

14 in total

1. DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY.

Authors: L ORNSTEIN
Journal: Ann N Y Acad Sci Date: 1964-12-28 Impact factor: 5.691

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Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

3. Blue Sepharose: a reusable affinity chromatography medium for purification of alcohol dehydrogenase.

Authors: G E Lamkin; E E King
Journal: Biochem Biophys Res Commun Date: 1976-09-20 Impact factor: 3.575

4. Isolation of albumin from whole human plasma and fractionation of albumin-depleted plasma.

Authors: J Travis; J Bowen; D Tewksbury; D Johnson; R Pannell
Journal: Biochem J Date: 1976-08-01 Impact factor: 3.857

5. Electrophoretic desorption of affinity adsorbents [proceedings].

Authors: P D Dean; P Brown; M J Leyland; D H Watson; S Angal; M J Harvey
Journal: Biochem Soc Trans Date: 1977 Impact factor: 5.407

6. Rapid purification of lactate dehydrogenase from rat liver and hepatoma: a new approach.

Authors: L D Ryan; C S Vestling
Journal: Arch Biochem Biophys Date: 1974-01 Impact factor: 4.013

7. Some parameters relevant to affinity chromatography on immobilized nucleotides.

Authors: C R Lowe; M J Harvey; D B Craven; P D Dean
Journal: Biochem J Date: 1973-07 Impact factor: 3.857

8. [The chromophore of blue dextran: a powerful ligand for the purification of interferon by affinity chromatography].

Authors: J de Maeyer-Guignard; E de Maeyer
Journal: C R Acad Hebd Seances Acad Sci D Date: 1976-09-27

9. Applications of blue dextran and Cibacron Blue F3GA in purification and structural studies of nucleotide-requiring enzymes.

Authors: J E Wilson
Journal: Biochem Biophys Res Commun Date: 1976-10-04 Impact factor: 3.575

10. A 3(17)beta-hydroxysteroid dehydrogenase in raterythrocytes. Conversion of 5alpha-dihydrotestosterone into 5alpha-androstane-3beta,17beta-diol and purification of the enzyme by affinity chromatography.

Authors: W Heyns; P De Moor
Journal: Biochim Biophys Acta Date: 1974-07-17

7 in total

1. Comparative studies of the binding of some ligands to human serum albumin non-covalently attached to immobilized Cibacron Blue, or covalently immobilized on Sepharose, by column affinity chromatography.

Authors: C Lagercrantz; T Larsson
Journal: Biochem J Date: 1983-08-01 Impact factor: 3.857

2. Chromatography of plasma proteins on immobilized Cibacron Blue F3-GA. Mechanism of the molecular interaction.

Authors: E Gianazza; P Arnaud
Journal: Biochem J Date: 1982-06-01 Impact factor: 3.857

3. A general method for fractionation of plasma proteins. Dye-ligand affinity chromatography on immobilized Cibacron blue F3-GA.

Authors: E Gianazza; P Arnaud
Journal: Biochem J Date: 1982-01-01 Impact factor: 3.857

4. Tandem dye-ligand chromatography and biospecific elution applied to the purification of glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides.

Authors: Y Hey; P D Dean
Journal: Biochem J Date: 1983-02-01 Impact factor: 3.857