Literature DB >> 8248781

Photoactivated conformational changes in rhodopsin: a time-resolved spin label study.

Z T Farahbakhsh1, K Hideg, W L Hubbell.   

Abstract

Rhodopsin has been selectively spin-labeled near the cytoplasmic termini of helices C and G. Photoactivation with a light flash induces an electron paramagnetic resonance spectral change in the millisecond time domain, coincident with the appearance of the active metarhodopsin II intermediate. The spectral change is consistent with a small movement near the cytoplasmic termination of the C helix and reverses upon formation of the MIII state. These results provide an important link between the optical changes associated with the retinal chromophore and protein conformational states.

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Year:  1993        PMID： 8248781     DOI： 10.1126/science.8248781

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  31 in total

1.  Solution 19F nuclear Overhauser effects in structural studies of the cytoplasmic domain of mammalian rhodopsin.

Authors:  M C Loewen; J Klein-Seetharaman; E V Getmanova; P J Reeves; H Schwalbe; H G Khorana
Journal:  Proc Natl Acad Sci U S A       Date:  2001-04-24       Impact factor: 11.205

2.  Time-resolved rhodopsin activation currents in a unicellular expression system.

Authors:  J M Sullivan; P Shukla
Journal:  Biophys J       Date:  1999-09       Impact factor: 4.033

3.  Agonist-induced conformational changes in the G-protein-coupling domain of the beta 2 adrenergic receptor.

Authors:  P Ghanouni; J J Steenhuis; D L Farrens; B K Kobilka
Journal:  Proc Natl Acad Sci U S A       Date:  2001-05-15       Impact factor: 11.205

4.  Structural insights into human GPCR protein OA1: a computational perspective.

Authors:  Anirban Ghosh; Uddhavesh Sonavane; Sai Krishna Andhirka; Gopala Krishna Aradhyam; Rajendra Joshi
Journal:  J Mol Model       Date:  2011-09-22       Impact factor: 1.810

Review 5.  G protein coupled receptor structure and activation.

Authors:  Brian K Kobilka
Journal:  Biochim Biophys Acta       Date:  2006-11-15

6.  Sequence of late molecular events in the activation of rhodopsin.

Authors:  Bernhard Knierim; Klaus Peter Hofmann; Oliver P Ernst; Wayne L Hubbell
Journal:  Proc Natl Acad Sci U S A       Date:  2007-12-11       Impact factor: 11.205

Review 7.  Spin labeling EPR.

Authors:  Johann P Klare; Heinz-Jürgen Steinhoff
Journal:  Photosynth Res       Date:  2009-08-29       Impact factor: 3.573

8.  Refolding of the integral membrane protein light-harvesting complex II monitored by pulse EPR.

Authors:  Christoph Dockter; Aleksei Volkov; Christian Bauer; Yevhen Polyhach; Zoé Joly-Lopez; Gunnar Jeschke; Harald Paulsen
Journal:  Proc Natl Acad Sci U S A       Date:  2009-10-15       Impact factor: 11.205

9.  Light-induced exposure of the cytoplasmic end of transmembrane helix seven in rhodopsin.

Authors:  N G Abdulaev; K D Ridge
Journal:  Proc Natl Acad Sci U S A       Date:  1998-10-27       Impact factor: 11.205

10.  Structure and function in rhodopsin: rhodopsin mutants with a neutral amino acid at E134 have a partially activated conformation in the dark state.

Authors:  J M Kim; C Altenbach; R L Thurmond; H G Khorana; W L Hubbell
Journal:  Proc Natl Acad Sci U S A       Date:  1997-12-23       Impact factor: 11.205
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