Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Fractionation of RNA from tetahymena by affinity chromatography on poly-U-Sepharose.

Literature DB >> 823341

Fractionation of RNA from tetahymena by affinity chromatography on poly-U-Sepharose.

Abstract

Exponential growing Tetrahymena pyriformis organisms were labelled with (3H) uridine or (3H) adenosine. The labelled RNA was extracted and isolated by affinity chromatography on poly-uridylic-acid sepharose and further analysed by means of sucrose gradient centrifugation and RNase digestion. Experimental evidence proved the existence of RNase resistant poly adenylic-acid fragments in the RNA of Tetrahymena cells. This poly adenylic-acid segment has a sedimentation rate of 4-5 S and would be localised in the 10-12S region of the RNA which is probably the m-RNA.

Entities: Chemical Species

Mesh：

Substances：
Poly A
Poly U
RNA

Year: 1976 PMID： 823341 DOI： 10.1007/BF01739099

Source DB: PubMed Journal: J Mol Evol ISSN： 0022-2844 Impact factor: 2.395

14 in total

1. Polyadenylic acid sequences in yeast messenger ribonucleic acid.

Authors: C S McLaughlin; J R Warner; M Edmonds; H Nakazato; M H Vaughan
Journal: J Biol Chem Date: 1973-02-25 Impact factor: 5.157

2. Lack of polyadenylic acid sequences in the messenger RNA of E. coli.

Authors: R P Perry; D E Kelley; J LaTorre
Journal: Biochem Biophys Res Commun Date: 1972-09-26 Impact factor: 3.575

3. Isolation and hybridization kinetics of messenger RNA from Dictyostelium discoideum.

Authors: R A Firtel; A Jacobson; H F Lodish
Journal: Nat New Biol Date: 1972-10-25

4. Isolation of mRNA from KB-cells by affinity chromatography on polyuridylic acid covalently linked to Sepharose.

Authors: U Lindberg; T Persson
Journal: Eur J Biochem Date: 1972-12-04

5. Addition of polyadenylate sequences to virus-specific RNA during adenovirus replication.

Authors: L Philipson; R Wall; G Glickman; J E Darnell
Journal: Proc Natl Acad Sci U S A Date: 1971-11 Impact factor: 11.205

6. Ribonucleic acid synthesis in vaccinia virus. II. Synthesis of polyriboadenylic acid.

Authors: J Kates; J Beeson
Journal: J Mol Biol Date: 1970-05-28 Impact factor: 5.469

7. An adenylic acid-rich sequence in messenger RNA of HeLa cells and its possible relationship to reiterated sites in DNA.

Authors: J E Darnell; R Wall; R J Tushinski
Journal: Proc Natl Acad Sci U S A Date: 1971-06 Impact factor: 11.205

8. A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells.

Authors: S Y Lee; J Mendecki; G Brawerman
Journal: Proc Natl Acad Sci U S A Date: 1971-06 Impact factor: 11.205

Fractionation of RNA from tetahymena by affinity chromatography on poly-U-Sepharose.

1. Polyadenylic acid sequences in yeast messenger ribonucleic acid.

2. Lack of polyadenylic acid sequences in the messenger RNA of E. coli.

3. Isolation and hybridization kinetics of messenger RNA from Dictyostelium discoideum.

4. Isolation of mRNA from KB-cells by affinity chromatography on polyuridylic acid covalently linked to Sepharose.

5. Addition of polyadenylate sequences to virus-specific RNA during adenovirus replication.

6. Ribonucleic acid synthesis in vaccinia virus. II. Synthesis of polyriboadenylic acid.

7. An adenylic acid-rich sequence in messenger RNA of HeLa cells and its possible relationship to reiterated sites in DNA.

8. A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells.

9. Polyadenylic acid in the cytoplasm of rat liver.

10. Messenger and heterogeneous nuclear RNA in HeLa cells: differential inhibition by cordycepin.

1. Increased RNA synthesis during preconjugation and its effect on pair formation in tetrahymena.

2. RNA and macronuclear transcription in the ciliate Stylonychia mytilus.