Literature DB >> 5288382

A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells.

S Y Lee, J Mendecki, G Brawerman.   

Abstract

The rapidly-labeled polyribosomal RNA component from mouse sarcoma 180 cells is retained on nitrocellulose (Millipore) membrane-filters at high ionic strength. This property is due to the presence of a polynucleotide sequence rich in adenylic acid that resists both T(1) and pancreatic RNase digestion. The resistant material shows sedimentation characteristics close to those of transfer RNA. The RNA molecules that contain this material can be separated from the rest of the polysomal RNA by differential phenol extraction with neutral and alkaline Tris buffers. Synthetic poly(A) exhibits the same behavior as the rapidly-labeled polysomal RNA with respect to Millipore binding and phenol fractionation. The characteristics of the rapidly-labeled polysomal RNA component permit its isolation free of ribosomal RNA.

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Year:  1971        PMID： 5288382      PMCID： PMC389183          DOI： 10.1073/pnas.68.6.1331

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  20 in total

1.  Cytoplasmic particles carrying rapidly labeled RNA in developing insect epidermis.

Authors:  F C Kafatos
Journal:  Proc Natl Acad Sci U S A       Date:  1968-04       Impact factor: 11.205

2.  Adenine-rich polymer associated with rabbit reticulocyte messenger RNA.

Authors:  L Lim; E S Canellakis
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

3.  Pulse-labeled ribonucleic acid complexes released by dissociation of rat liver polysomes.

Authors:  S Y Lee; G Brawerman
Journal:  Biochemistry       Date:  1971-02-02       Impact factor: 3.162

4.  The isolation and characterization of adenosine monophosphate-rich polynucleotides synthesized by Ehrlich ascites cells.

Authors:  M Edmonds; M G Caramela
Journal:  J Biol Chem       Date:  1969-03-10       Impact factor: 5.157

5.  Selective inhibition of the synthesis of mitochondria-associated RNA by ethidium bromide.

Authors:  E Zylber; C Vesco; S Penman
Journal:  J Mol Biol       Date:  1969-08-28       Impact factor: 5.469

6.  Unexpectedly large size of globin messenger ribonucleic acid.

Authors:  P Gaskill; D Kabat
Journal:  Proc Natl Acad Sci U S A       Date:  1971-01       Impact factor: 11.205

7.  Messenger RNA-protein complexes and newly synthesized ribosomal subunits: analysis of free particles and components of polyribosomes.

Authors:  R P Perry; D E Kelley
Journal:  J Mol Biol       Date:  1968-07-14       Impact factor: 5.469

8.  Messenger RNA in rat liver polyribosomes: evidence that it exists as ribonucleoprotein particles.

Authors:  E C Henshaw
Journal:  J Mol Biol       Date:  1968-09-28       Impact factor: 5.469

9.  Polyadenylic acid in the cytoplasm of rat liver.

Authors:  A Hadjivassiliou; G Brawerman
Journal:  J Mol Biol       Date:  1966-09       Impact factor: 5.469

10.  Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship.

Authors:  M Edmonds; M H Vaughan; H Nakazato
Journal:  Proc Natl Acad Sci U S A       Date:  1971-06       Impact factor: 11.205
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  163 in total

1.  Lack of poly(A) sequence in half of the messenger RNA coding for ewe alpha S casein.

Authors:  L M Houdebine; P Gaye; A Favre
Journal:  Nucleic Acids Res       Date:  1974-03       Impact factor: 16.971

2.  Messenger activity of ribonucleic acid form yeast mitochondria.

Authors:  M J Eggitt; A H Scragg
Journal:  Biochem J       Date:  1975-09       Impact factor: 3.857

3.  Kinetics of induced and repressed enzyme synthesis in Saccharomyces cerevisiae.

Authors:  R P Lawther; T G Cooper
Journal:  J Bacteriol       Date:  1975-03       Impact factor: 3.490

4.  Synthesis of polyadenylic acid-containing ribonucleic acid during the germination of Neurospora crassa conidia.

Authors:  P E Mirkes; B McCalley
Journal:  J Bacteriol       Date:  1976-01       Impact factor: 3.490

5.  E.B. Wilson Lecture, 1998. Eukaryotic RNAs: once more from the beginning.

Authors:  J E Darnell
Journal:  Mol Biol Cell       Date:  1999-06       Impact factor: 4.138

6.  Hybridization of polyuridylic acid to human DNA immobilized onto nitrocellulose filters.

Authors:  R W Pero; T Bryngelsson; C Bryngelsson; A Deutsch; A Nordén; A Norgren
Journal:  Nucleic Acids Res       Date:  1975-07       Impact factor: 16.971

7.  Absence of mRNA for casein in free polysomes of lactating ewe mammary gland.

Authors:  L M Houdebine; P Gaye
Journal:  Nucleic Acids Res       Date:  1975-02       Impact factor: 16.971

8.  Isolation and characterization of casein mRNAs from lactating ewe mammary glands.

Authors:  P Gaye; L M Houdebine
Journal:  Nucleic Acids Res       Date:  1975-05       Impact factor: 16.971

9.  Ribosomal and informational ribonucleoprotein complexes of animal cells. Study on rat liver ribonucleic acids as constituents of ribonucleoprotein complexes by chromatography on nucleoprotein-celite columns.

Authors:  A V Lichtenstein; R P Alechina; V S Shapot
Journal:  Biochem J       Date:  1975-06       Impact factor: 3.857

10.  Origin of reovirus oligo(A).

Authors:  C Carter; C M Stoltzfus; A K Banerjee; A J Shatkin
Journal:  J Virol       Date:  1974-06       Impact factor: 5.103
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