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Literature DB >> 8228289

Analysis of active antibody concentration. Separation of affinity and concentration parameters.

R Karlsson¹, L Fägerstam, H Nilshans, B Persson.

Abstract

Antibody binding to surfaces with differing amounts of immobilised antigen was measured in a biosensor system using surface plasmon resonance detection. Binding rates obtained during the initial binding phase on high density antigen surfaces were proportional to antibody concentration and independent of antigen-antibody affinity. One antibody calibration curve covering the range from 0.5 to 160 nM (0.08-25 micrograms/ml) antibody was valid for IgG antibodies with different antigen specificities. To illustrate the use of this methodology active antibody concentrations were analysed in culture media and in rabbit serum.

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Year: 1993 PMID： 8228289 DOI： 10.1016/0022-1759(93)90330-a

Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303

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Cited

11 in total

1. Resonant mirror biosensor analysis of type Ialpha cAMP-dependent protein kinase B domain--cyclic nucleotide interactions.

Authors: W W Muhonen; J B Shabb
Journal: Protein Sci Date: 2000-12 Impact factor: 6.725

2. Functional aspects of co-variant surface charges in an antibody fragment.

Authors: Nicolas Hugo; Virginie Lafont; Mervyn Beukes; Danièle Altschuh
Journal: Protein Sci Date: 2002-11 Impact factor: 6.725

3. Nonregeneration protocol for surface plasmon resonance: study of high-affinity interaction with high-density biosensors.

Authors: Yijun Tang; Ray Mernaugh; Xiangqun Zeng
Journal: Anal Chem Date: 2006-03-15 Impact factor: 6.986

Review 4. Biosensor binding data and its applicability to the determination of active concentration.

Authors: Robert Karlsson
Journal: Biophys Rev Date: 2016-10-17

5. IgM anti-myeloperoxidase antibody-secreting lymphocytes are present in the peripheral repertoire of lupus mice but rarely differentiate into IgG-producing cells.

Authors: O Vittecoq; F Brard; F Jovelin; X Le Loet; F Tron; D Gilbert
Journal: Clin Exp Immunol Date: 1999-10 Impact factor: 4.330

Analysis of active antibody concentration. Separation of affinity and concentration parameters.

1. Resonant mirror biosensor analysis of type Ialpha cAMP-dependent protein kinase B domain--cyclic nucleotide interactions.

2. Functional aspects of co-variant surface charges in an antibody fragment.

3. Nonregeneration protocol for surface plasmon resonance: study of high-affinity interaction with high-density biosensors.

Review 4. Biosensor binding data and its applicability to the determination of active concentration.

5. IgM anti-myeloperoxidase antibody-secreting lymphocytes are present in the peripheral repertoire of lupus mice but rarely differentiate into IgG-producing cells.

6. Analysis of agalacto-IgG in rheumatoid arthritis using surface plasmon resonance.

7. Determining the Binding Kinetics of Peptide Macrocycles Using Bio-Layer Interferometry (BLI).

8. Two functional states of the CD11b A-domain: correlations with key features of two Mn2+-complexed crystal structures.

9. Isolation of high affinity human antibodies directly from large synthetic repertoires.

10. Solution-phase vs surface-phase aptamer-protein affinity from a label-free kinetic biosensor.