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Literature DB >> 822115

The Ly-3 antigens on mouse thymocytes: immune precipitation and molecular weight characterization.

Abstract

Specific anti-Ly sera were employed to precipitate Ly antigens from Nonidet P-40 extracts of mouse thymocytes labeled with 125I using lactoperoxidase and with NaB3H4 using galactose oxidase. Thymocytes from mice of the congenic strains C57BL/6J (Ly-2.2, Ly-3.2 positive), C57BL/6Ly-2a, Ly-3a (Ly-2.1, Ly-3.1 positive) and C57BL/6-Ly-2a (Ly-2.1, Ly-3.2-positive) were used as sources of labeled antigens and as immune adsorbants to permit evaluation of the specificity of each anti-Ly serum employed. Results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions are consistent with the Ly-3.1 antigen containing a glycoprotein subunit with an apparent mol wt of 35,000 daltons. Specific precipitates obtained using anti-Ly-2.1 serum yielded SDS-PAGE profiles identical to that obtained with anti-Ly-3.1 serum, suggesting that the Ly-2 and Ly-3 antigens have the same molecular weight distribution. The relationships of these results to the observed close genetic and topological linkage of Ly-2 and Ly-3 and to the genetic linkage of these loci with the IB-peptide marker, a mouse Bk-region polymorphism, are discussed.

Entities: CellLine Chemical Gene Species

Mesh：

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Year: 1976 PMID： 822115 PMCID： PMC2190382 DOI： 10.1084/jem.144.2.476

Source DB: PubMed Journal: J Exp Med ISSN： 0022-1007 Impact factor: 14.307

30 in total

1. Human erythrocyte membrane glycoprotein: a re-evaluation of the molecular weight as determined by SDS polyacrylamide gel electrophoresis.

Authors: J P Segrest; R L Jackson; E P Andrews; V T Marchesi
Journal: Biochem Biophys Res Commun Date: 1971-07-16 Impact factor: 3.575

2. Ly-A and Ly-B: two systems of lymphocyte isoantigens in the mouse.

Authors: E A Boyse; M Miyazawa; T Aoki; L J Old
Journal: Proc R Soc Lond B Biol Sci Date: 1968-06-11

3. Membrane molecules determined by the H-2 associated immune response region: isolation and some properties.

Authors: S E Cullen; C S David; D C Shreffler; S G Nathenson
Journal: Proc Natl Acad Sci U S A Date: 1974-03 Impact factor: 11.205

4. The covalent structure of a human gamma G-immunoglobulin. X. Intrachain disulfide bonds.

Authors: W E Gall; G M Edelman
Journal: Biochemistry Date: 1970-08-04 Impact factor: 3.162

5. Association of a beta2-microglobulin-like subunit with H-2 and TL alloantigens on murine thymocytes.

Authors: E S Vitetta; J W Uhr; E A Boyse
Journal: J Immunol Date: 1975-01 Impact factor: 5.422

6. Subunit structure, cell surface orientation, and partial amino-acid sequences of murine histocompatibility antigens.

Authors: R Henning; R J Milner; K Reske; B A Cunningham; G M Edelman
Journal: Proc Natl Acad Sci U S A Date: 1976-01 Impact factor: 11.205

7. Cell surface immunoglobulin. IV. Distribution among thymocytes, bone mrrow cells, and their derived populations.

Authors: E S Vitetta; C Bianco; V Nussenzweig; J W Uhr
Journal: J Exp Med Date: 1972-07-01 Impact factor: 14.307

8. Properties of antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. I. In vivo activity and immunochemical characterization.

Authors: T Takemori; T Tada
Journal: J Exp Med Date: 1975-11-01 Impact factor: 14.307

9. Functional subclasses of T lymphocytes bearing different Ly antigens. II. Cooperation between subclasses of Ly+ cells in the generation of killer activity.

Authors: H Cantor; E A Boyse
Journal: J Exp Med Date: 1975-06-01 Impact factor: 14.307

10. Lyt-2 and lyt-3 antigens are on two different polypeptide subunits linked by disulfide bonds. Relationship of subunits to T cell cytolytic activity.

Authors: J A Ledbetter; W E Seaman; T T Tsu; L A Herzenberg
Journal: J Exp Med Date: 1981-06-01 Impact factor: 14.307

The Ly-3 antigens on mouse thymocytes: immune precipitation and molecular weight characterization.

1. Human erythrocyte membrane glycoprotein: a re-evaluation of the molecular weight as determined by SDS polyacrylamide gel electrophoresis.

2. Ly-A and Ly-B: two systems of lymphocyte isoantigens in the mouse.

3. Membrane molecules determined by the H-2 associated immune response region: isolation and some properties.

4. The covalent structure of a human gamma G-immunoglobulin. X. Intrachain disulfide bonds.

5. Association of a beta2-microglobulin-like subunit with H-2 and TL alloantigens on murine thymocytes.

6. Subunit structure, cell surface orientation, and partial amino-acid sequences of murine histocompatibility antigens.

7. Cell surface immunoglobulin. IV. Distribution among thymocytes, bone mrrow cells, and their derived populations.

8. Properties of antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. I. In vivo activity and immunochemical characterization.

9. Functional subclasses of T lymphocytes bearing different Ly antigens. II. Cooperation between subclasses of Ly+ cells in the generation of killer activity.

10. Genetic correlation of a mouse light chain variable region marker with a thymocyte surface antigen.

1. Inducible functions in hybrids of a Lyt-2+ BW5147 transfectant and the 2C CTL line.

2. Differential susceptibility of mouse Lyt-2 and Lyt-3 genes to negative regulation.

3. Effects of Lyt antibodies on T-cell functions: augmentation by anti-Lyt-1 as opposed to inhibition by anti-Lyt-2.

4. Construction and properties of new Lyt-congenic strains and anti-Lyt-2.2 and anti-Lyt-3.1 monoclonal antibodies.

5. Surface glycoproteins of resting and activated human T lymphocytes.

6. Structure and expression of the Lyt-3a gene of C.AKR mice.

7. Mouse Lyt-2 antigen: evidence for two heterodimers with a common subunit.

8. Purification, chain separation and sequence of the MRC OX-8 antigen, a marker of rat cytotoxic T lymphocytes.

9. Lyt-2-/Lyt 3- variants of a cloned cytolytic T cell line lack an antigen receptor functional in cytolysis.

10. Lyt-2 and lyt-3 antigens are on two different polypeptide subunits linked by disulfide bonds. Relationship of subunits to T cell cytolytic activity.