Literature DB >> 8190066

Cloning and characterization of the gene encoding an esterase from Spirulina platensis.

S Salvi1, M Trinei, L Lanfaloni, C L Pon.   

Abstract

The gene encoding a 23 kDA serine esterase from the cyanobacterium Spirulina platensis has been identified, cloned, characterized and expressed in Escherichia coli. The primary structure of the esterase deduced from the DNA sequence displayed 32% sequence identity with the carboxylesterase (esterase II) encoded by estB of Pseudomonas fluorescens; the highest degree of homology is found in a stretch of 11 identical or highly conserved amino acid residues corresponding to the GXSXG consensus motif found in the catalytic site of many serine proteases, lipases and esterases.

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Year:  1994        PMID： 8190066     DOI： 10.1007/bf00283885

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  10 in total

1.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

2.  Molecular cloning and nucleotide sequence of the lipase gene from Pseudomonas fragi.

Authors:  W Kugimiya; Y Otani; Y Hashimoto; Y Takagi
Journal:  Biochem Biophys Res Commun       Date:  1986-11-26       Impact factor: 3.575

Review 3.  The biochemistry and industrial potential of Spirulina.

Authors:  O Ciferri; O Tiboni
Journal:  Annu Rev Microbiol       Date:  1985       Impact factor: 15.500

4.  Plasmid vectors for high-efficiency expression controlled by the PL promoter of coliphage lambda.

Authors:  E Remaut; P Stanssens; W Fiers
Journal:  Gene       Date:  1981-10       Impact factor: 3.688

5.  Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.

Authors:  F Sanger; A R Coulson; B G Barrell; A J Smith; B A Roe
Journal:  J Mol Biol       Date:  1980-10-25       Impact factor: 5.469

Review 6.  Spirulina, the edible microorganism.

Authors:  O Ciferri
Journal:  Microbiol Rev       Date:  1983-12

7.  Structure of the canine pancreatic lipase gene.

Authors:  F S Mickel; F Weidenbach; B Swarovsky; K S LaForge; G A Scheele
Journal:  J Biol Chem       Date:  1989-08-05       Impact factor: 5.157

8.  Molecular cloning and characterization of esterase-6, a serine hydrolase of Drosophila.

Authors:  J G Oakeshott; C Collet; R W Phillis; K M Nielsen; R J Russell; G K Chambers; V Ross; R C Richmond
Journal:  Proc Natl Acad Sci U S A       Date:  1987-05       Impact factor: 11.205

9.  The molecular evolution of genes and proteins: a tale of two serines.

Authors:  S Brenner
Journal:  Nature       Date:  1988-08-11       Impact factor: 49.962

10.  Characterization of Pseudomonas fluorescens carboxylesterase: cloning and expression of the esterase gene in Escherichia coli.

Authors:  K H Hong; W H Jang; K D Choi; O J Yoo
Journal:  Agric Biol Chem       Date:  1991-11
  10 in total
  2 in total

1.  Bacterial lipolytic enzymes: classification and properties.

Authors:  J L Arpigny; K E Jaeger
Journal:  Biochem J       Date:  1999-10-01       Impact factor: 3.857

2.  Molecular analysis of the Rhodococcus sp. strain H1 her gene and characterization of its product, a heroin esterase, expressed in Escherichia coli.

Authors:  D A Rathbone; P J Holt; C R Lowe; N C Bruce
Journal:  Appl Environ Microbiol       Date:  1997-05       Impact factor: 4.792
  2 in total

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