Literature DB >> 9143135

Molecular analysis of the Rhodococcus sp. strain H1 her gene and characterization of its product, a heroin esterase, expressed in Escherichia coli.

D A Rathbone1, P J Holt, C R Lowe, N C Bruce.   

Abstract

The structural gene for heroin esterase was cloned from Rhodococcus sp. strain H1 and expressed in Escherichia coli BL21(DE3). The purified enzyme was found to be a tetramer with an M(r) of 137,000 and an apparent K(m) of 0.88 mM for 6-acetylmorphine. The G-x-S-x-G motif was observed in the deduced amino acid sequence, suggesting that the enzyme is a serin esterase.

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Year:  1997        PMID： 9143135      PMCID： PMC168495          DOI： 10.1128/aem.63.5.2062-2066.1997

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  20 in total

1.  Towards engineering pathways for the synthesis of analgesics and antitussives.

Authors:  N C Bruce; D A Caswell; C E French; A M Hailes; M T Long; D L Willey
Journal:  Ann N Y Acad Sci       Date:  1994-05-02       Impact factor: 5.691

2.  Bioluminescent assay for heroin and its metabolites.

Authors:  P J Holt; N C Bruce; C R Lowe
Journal:  Anal Chem       Date:  1996-06-01       Impact factor: 6.986

3.  The use of recombinant DNA technology in the design of a highly specific heroin sensor.

Authors:  D A Rathbone; P J Holt; N C Bruce; C R Lowe
Journal:  Ann N Y Acad Sci       Date:  1996-05-15       Impact factor: 5.691

4.  The use of a novel recombinant heroin esterase in the development of an illicit drugs biosensor.

Authors:  D A Rathbone; P J Holt; C R Lowe; N C Bruce
Journal:  Ann N Y Acad Sci       Date:  1996-10-12       Impact factor: 5.691

5.  Cloning and characterization of the gene encoding an esterase from Spirulina platensis.

Authors:  S Salvi; M Trinei; L Lanfaloni; C L Pon
Journal:  Mol Gen Genet       Date:  1994-04

6.  Identification of a heroin esterase in Rhodococcus sp. strain H1.

Authors:  G W Cameron; K N Jordan; P J Holt; P B Baker; C R Lowe; N C Bruce
Journal:  Appl Environ Microbiol       Date:  1994-10       Impact factor: 4.792

7.  Molecular characterization of the extracellular poly(3-hydroxyoctanoic acid) [P(3HO)] depolymerase gene of Pseudomonas fluorescens GK13 and of its gene product.

Authors:  A Schirmer; D Jendrossek
Journal:  J Bacteriol       Date:  1994-11       Impact factor: 3.490

8.  An amperometric opiate assay.

Authors:  P J Holt; L D Stephens; N C Bruce; C R Lowe
Journal:  Biosens Bioelectron       Date:  1995       Impact factor: 10.618

9.  Nucleotide sequence and over-expression of morphine dehydrogenase, a plasmid-encoded gene from Pseudomonas putida M10.

Authors:  D L Willey; D A Caswell; C R Lowe; N C Bruce
Journal:  Biochem J       Date:  1993-03-01       Impact factor: 3.857

10.  The peptide synthetase gene phsA from Streptomyces viridochromogenes is not juxtaposed with other genes involved in nonribosomal biosynthesis of peptides.

Authors:  D Schwartz; R Alijah; B Nussbaumer; S Pelzer; W Wohlleben
Journal:  Appl Environ Microbiol       Date:  1996-02       Impact factor: 4.792
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  4 in total

Review 1.  Genome of the extremely radiation-resistant bacterium Deinococcus radiodurans viewed from the perspective of comparative genomics.

Authors:  K S Makarova; L Aravind; Y I Wolf; R L Tatusov; K W Minton; E V Koonin; M J Daly
Journal:  Microbiol Mol Biol Rev       Date:  2001-03       Impact factor: 11.056

2.  Isolation and biochemical characterization of two novel metagenome-derived esterases.

Authors:  C Elend; C Schmeisser; C Leggewie; P Babiak; J D Carballeira; H L Steele; J-L Reymond; K-E Jaeger; W R Streit
Journal:  Appl Environ Microbiol       Date:  2006-05       Impact factor: 4.792

3.  Rhodococcus sp. strain CR-53 LipR, the first member of a new bacterial lipase family (family X) displaying an unusual Y-type oxyanion hole, similar to the Candida antarctica lipase clan.

Authors:  Arnau Bassegoda; F I Javier Pastor; Pilar Diaz
Journal:  Appl Environ Microbiol       Date:  2012-01-06       Impact factor: 4.792

4.  Screening, nucleotide sequence, and biochemical characterization of an esterase from Pseudomonas fluorescens with high activity towards lactones.

Authors:  V Khalameyzer; I Fischer; U T Bornscheuer; J Altenbuchner
Journal:  Appl Environ Microbiol       Date:  1999-02       Impact factor: 4.792
  4 in total

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