Literature DB >> 8177738

The DNA-dependent RNA-polymerase of Thermotoga maritima; characterisation of the enzyme and the DNA-sequence of the genes for the large subunits.

P Palm1, C Schleper, I Arnold-Ammer, I Holz, T Meier, F Lottspeich, W Zillig.   

Abstract

An improved purification procedure for Thermotoga maritima RNA-polymerase holoenzyme was developed. The enzyme is highly active with poly dAT or T7 phage DNA as template. DNA gyrase was found to be a side product of this RNA-polymerase purification. The genes for the large subunits beta and beta' of RNA-polymerase were cloned and sequenced. The phylogenetic position of T.maritima within the bacterial domain was determined by various methods. It is the lowest bacterial offspring but slightly higher than the chloroplasts.

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Year:  1993        PMID： 8177738      PMCID： PMC311404          DOI： 10.1093/nar/21.21.4904

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  35 in total

1.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

2.  DNA-dependent RNA polymerase from the archaebacterium Sulfolobus acidocaldarius.

Authors:  W Zillig; K O Stetter; D Janeković
Journal:  Eur J Biochem       Date:  1979-06-01

3.  Isolation of preribosomes from HeLa cells and their characterization by electrophoresis on uniform and exponential-gradient-polyacrylamide gels.

Authors:  M E Mirault; K Scherrer
Journal:  Eur J Biochem       Date:  1971-11-11

4.  Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.

Authors:  F Sanger; A R Coulson; B G Barrell; A J Smith; B A Roe
Journal:  J Mol Biol       Date:  1980-10-25       Impact factor: 5.469

5.  A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.

Authors:  J Messing; J Vieira
Journal:  Gene       Date:  1982-10       Impact factor: 3.688

6.  The primary structure of E. coli RNA polymerase, Nucleotide sequence of the rpoC gene and amino acid sequence of the beta'-subunit.

Authors:  G S Monastyrskaya; V V Gubanov; S O Guryev; I S Salomatina; T M Shuvaeva; V M Lipkin; E D Sverdlov
Journal:  Nucleic Acids Res       Date:  1982-07-10       Impact factor: 16.971

7.  A comprehensive set of sequence analysis programs for the VAX.

Authors:  J Devereux; P Haeberli; O Smithies
Journal:  Nucleic Acids Res       Date:  1984-01-11       Impact factor: 16.971

8.  An improved strategy for rapid direct sequencing of both strands of long DNA molecules cloned in a plasmid.

Authors:  L H Guo; R C Yang; R Wu
Journal:  Nucleic Acids Res       Date:  1983-08-25       Impact factor: 16.971

9.  A new computer method for the storage and manipulation of DNA gel reading data.

Authors:  R Staden
Journal:  Nucleic Acids Res       Date:  1980-08-25       Impact factor: 16.971

10.  The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.

Authors:  J Shine; L Dalgarno
Journal:  Proc Natl Acad Sci U S A       Date:  1974-04       Impact factor: 11.205
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  4 in total

1.  A NusG-like protein from Thermotoga maritima binds to DNA and RNA.

Authors:  D Liao; R Lurz; B Dobrinski; P P Dennis
Journal:  J Bacteriol       Date:  1996-07       Impact factor: 3.490

2.  Both DNA gyrase and reverse gyrase are present in the hyperthermophilic bacterium Thermotoga maritima.

Authors:  O Guipaud; E Marguet; K M Noll; C B de la Tour; P Forterre
Journal:  Proc Natl Acad Sci U S A       Date:  1997-09-30       Impact factor: 11.205

3.  Sequencing of heat shock protein 70 (DnaK) homologs from Deinococcus proteolyticus and Thermomicrobium roseum and their integration in a protein-based phylogeny of prokaryotes.

Authors:  R S Gupta; K Bustard; M Falah; D Singh
Journal:  J Bacteriol       Date:  1997-01       Impact factor: 3.490

4.  In vitro transcription close to the melting point of DNA: analysis of Thermotoga maritima RNA polymerase-promoter complexes at 75 degrees C using chemical probes.

Authors:  T Meier; P Schickor; A Wedel; L Cellai; H Heumann
Journal:  Nucleic Acids Res       Date:  1995-03-25       Impact factor: 16.971
  4 in total

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