Literature DB >> 8151798

Purification of recombinant budgerigar fledgling disease virus VP1 capsid protein and its ability for in vitro capsid assembly.

R E Rodgers1, D Chang, X Cai, R A Consigli.   

Abstract

A recombinant system for the major capsid VP1 protein of budgerigar fledgling disease virus has been established. The VP1 gene was inserted into a truncated form of the pFlag-1 vector and expressed in Escherichia coli. The budgerigar fledgling disease virus VP1 protein was purified to near homogeneity by immunoaffinity chromatography. Fractions containing highly purified VP1 were pooled and found to constitute 3.3% of the original E. coli-expressed VP1 protein. Electron microscopy revealed that the VP1 protein was isolated as pentameric capsomeres. Electron microscopy also revealed that capsid-like particles were formed in vitro from purified VP1 capsomeres with the addition of Ca2+ ions and the removal of chelating and reducing agents.

Entities:  

Keywords:  NASA Discipline Developmental Biology; Non-NASA Center

Mesh：

Substances：

Year:  1994        PMID： 8151798      PMCID： PMC236831     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  43 in total

1.  Chromatographic separation of the polyoma virus proteins and renaturation of the isolated VP1 major capsid protein.

Authors:  J N Brady; R A Consigli
Journal:  J Virol       Date:  1978-08       Impact factor: 5.103

2.  Immunological reactivity of antisera to sodium dodecyl sulfate-derived polypeptides of polyoma virions.

Authors:  J McMillen; R A Consigli
Journal:  J Virol       Date:  1977-03       Impact factor: 5.103

3.  Differences in the subpopulations of the structural proteins of polyoma virions and capsids: biological functions of the multiple VP1 species.

Authors:  J B Bolen; D G Anders; J Trempy; R A Consigli
Journal:  J Virol       Date:  1981-01       Impact factor: 5.103

4.  Comparison of nonphosphorylated and phosphorylated species of polyomavirus major capsid protein VP1 and identification of the major phosphorylation region.

Authors:  D G Anders; R A Consigli
Journal:  J Virol       Date:  1983-10       Impact factor: 5.103

5.  A viral disease of fledgling budgerigars.

Authors:  R B Davis; L H Bozeman; D Gaudry; O J Fletcher; P D Lukert; M J Dykstra
Journal:  Avian Dis       Date:  1981 Jan-Mar       Impact factor: 1.577

6.  In vitro reassembly of infectious polyoma virions.

Authors:  J N Brady; J D Kendall; R A Consigli
Journal:  J Virol       Date:  1979-11       Impact factor: 5.103

7.  A generalized inclusion body disease in the budgerigar (Melopsittacus undulatus) caused by a papovavirus-like agent.

Authors:  G Bernier; M Morin; G Marsolais
Journal:  Avian Dis       Date:  1981 Oct-Dec       Impact factor: 1.577

8.  Characterization of a papovavirus isolated from fledgling budgerigars.

Authors:  L H Bozeman; R B Davis; D Gaudry; P D Lukert; O J Fletcher; M J Dykstra
Journal:  Avian Dis       Date:  1981 Oct-Dec       Impact factor: 1.577

9.  Improved infectivity of reassembled polyoma virus.

Authors:  L K Yuen; R A Consigli
Journal:  J Virol       Date:  1982-07       Impact factor: 5.103

10.  Chemical cleavage of polyomavirus major structural protein VP1: identification of cleavage products and evidence that the receptor moiety resides in the carboxy-terminal region.

Authors:  D G Anders; R A Consigli
Journal:  J Virol       Date:  1983-10       Impact factor: 5.103
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  6 in total

1.  The structure of avian polyomavirus reveals variably sized capsids, non-conserved inter-capsomere interactions, and a possible location of the minor capsid protein VP4.

Authors:  Peter S Shen; Dirk Enderlein; Christian D S Nelson; Weston S Carter; Masaaki Kawano; Li Xing; Robert D Swenson; Norman H Olson; Timothy S Baker; R Holland Cheng; Walter J Atwood; Reimar Johne; David M Belnap
Journal:  Virology       Date:  2011-01-15       Impact factor: 3.616

2.  Structure and assembly of a T=1 virus-like particle in BK polyomavirus.

Authors:  Josefina Nilsson; Naoyuki Miyazaki; Li Xing; Bomu Wu; Lena Hammar; Tian Cheng Li; Naokazu Takeda; Tatsuo Miyamura; R Holland Cheng
Journal:  J Virol       Date:  2005-05       Impact factor: 5.103

3.  Hamster polyomavirus-derived virus-like particles are able to transfer in vitro encapsidated plasmid DNA to mammalian cells.

Authors:  Tatyana Voronkova; Andris Kazaks; Velta Ose; Muhsin Ozel; Siegfried Scherneck; Paul Pumpens; Rainer Ulrich
Journal:  Virus Genes       Date:  2006-08-22       Impact factor: 2.332

4.  Importance of calcium-binding site 2 in simian virus 40 infection.

Authors:  Peggy P Li; Albert P Nguyen; Qiumin Qu; Qumber H Jafri; Saharat Aungsumart; R Holland Cheng; Harumi Kasamatsu
Journal:  J Virol       Date:  2007-03-14       Impact factor: 5.103

5.  Nuclear localization of avian polyomavirus structural protein VP1 is a prerequisite for the formation of virus-like particles.

Authors:  Reimar Johne; Hermann Müller
Journal:  J Virol       Date:  2004-01       Impact factor: 5.103

6.  Production of recombinant VP1-derived virus-like particles from novel human polyomaviruses in yeast.

Authors:  Milda Norkiene; Jomante Stonyte; Danguole Ziogiene; Egle Mazeike; Kestutis Sasnauskas; Alma Gedvilaite
Journal:  BMC Biotechnol       Date:  2015-08-04       Impact factor: 2.563
  6 in total

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