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Literature DB >> 191638

Immunological reactivity of antisera to sodium dodecyl sulfate-derived polypeptides of polyoma virions.

Abstract

A study was undertaken to produce antisera to sodium dodecyl sulfate-derived polyoma virion polypeptides. With the use of this antisera, it was possible to detect, by immunofluorescence, cytoplasmic synthesis of V1, V2, and V3 polypeptides at 18 h postinfection and subsequent transport to the nucleus by 22 h postinfection. Anti-V1, anti-V2, and anti-V3 sera did not react with intact virions in an immunodiffusion assay, nor did they possess hemagglutination inhibition or viral neutralization activity. Antiserum produced against the four host histone polypeptides (V4 through V7) demonstrated immunofluorescence when reacted with polyoma-infected cells but not with uninfected cells. Antihistone serum was also capable of neutralizing viral infectivity, inhibiting hemagglutination and reacting with whole virions in an immunodiffusion assay.

Entities: Chemical Disease

Mesh：

Substances：

Year: 1977 PMID： 191638 PMCID： PMC515652

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

25 in total

10. Antibody to the type 5 adenovirus hexon polypeptide: detection of nascent polypeptides in the cytoplasm of infected KB cells.

Authors: M F Stinski; H S Ginsberg
Journal: Intervirology Date: 1974 Impact factor: 1.763

30 in total

6. Characterization of a DNA-protein complex and capsomere subunits derived from polyoma virus by treatment with ethyleneglycol-bis-N,N'-tetraacetic acid and dithiothreitol.

Authors: J N Brady; V D Winston; R A Consigli
Journal: J Virol Date: 1978-07 Impact factor: 5.103

Immunological reactivity of antisera to sodium dodecyl sulfate-derived polypeptides of polyoma virions.

1. PHYSICAL AND IMMUNOLOGICAL PROPERTIES OF A SOLUBLE PRECURSOR OF THE POLIOVIRUS CAPSID.

2. A simple chromatographic method for preparation of gamma globulin.

3. The serum proteins in multiple myelomatosis.

4. Origin of the polyoma virus-associated endonuclease.

5. Deficiency in histone acetylation in nontransforming host range mutants of polyoma virus.

6. Decapsidation of polyoma virus: identification of subviral species.

7. Polyoma virus strain with enhanced synthesis of capsid protein.

8. Protein measurement with the Folin phenol reagent.

9. Early events in polyoma virus infection: attachment, penetration, and nuclear entry.

10. Antibody to the type 5 adenovirus hexon polypeptide: detection of nascent polypeptides in the cytoplasm of infected KB cells.

1. Differential adsorption of polyoma virions and capsids to mouse kidney cells and guinea pig erythrocytes.

2. Intracellular localization of viral polypeptides during simian virus 40 infection.

3. Chromatographic separation of the polyoma virus proteins and renaturation of the isolated VP1 major capsid protein.

4. Production and characterization of monoclonal antibodies to polyomavirus major capsid protein VP1.

5. Localization of calcium on the polyomavirus VP1 capsid protein.

6. Characterization of a DNA-protein complex and capsomere subunits derived from polyoma virus by treatment with ethyleneglycol-bis-N,N'-tetraacetic acid and dithiothreitol.

7. Characterization of the DNA binding properties of polyomavirus capsid protein.

8. Isolation and characterization of polyoma uncoating intermediates from the nuclei of infected mouse cells.

9. Detection and quantitation of Newcastle disease virus proteins in infected chicken embryo cells.

10. Improved infectivity of reassembled polyoma virus.