Literature DB >> 8138127

A PCR-based assay for the identification of the fish pathogen Renibacterium salmoninarum.

G León1, N Maulén, J Figueroa, J Villanueva, C Rodríguez, M I Vera, M Krauskopf.   

Abstract

By means of a one-step one-tube extraction from less than 1 mg of tissue it is possible to identify, via the polymerase chain reaction, Renibacterium salmoninarum in salmon with bacterial kidney disease. A 149-bp DNA sequence unique to R. salmoninarum was specifically amplified and its nature confirmed by Southern hybridization using a non-isotopically labelled probe. The sensitivity of the approach allowed the detection of 22 R. salmoninarum cells. The procedure was successfully applied in the identification of the causative agent of bacterial kidney disease in kidney tissue from infected fishes.

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Year:  1994        PMID： 8138127     DOI： 10.1111/j.1574-6968.1994.tb06627.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  7 in total

1.  A simplified PCR-based method for the detection of Renibacterium salmoninarum utilizing preparations of rainbow trout (Oncorhynchus mykiss, Walbaum) lymphocytes.

Authors:  D McIntosh; P G Meaden; B Austin
Journal:  Appl Environ Microbiol       Date:  1996-11       Impact factor: 4.792

2.  A sensitive nested reverse transcriptase PCR assay to detect viable cells of the fish pathogen Renibacterium salmoninarum in Atlantic salmon (Salmo salar L.).

Authors:  M Cook; W H Lynch
Journal:  Appl Environ Microbiol       Date:  1999-07       Impact factor: 4.792

3.  PCR and probe-PCR assays to monitor broodstock Atlantic salmon (Salmo salar L.) ovarian fluid and kidney tissue for presence of DNA of the fish pathogen Renibacterium salmoninarum.

Authors:  A Miriam; S G Griffiths; J E Lovely; W H Lynch
Journal:  J Clin Microbiol       Date:  1997-06       Impact factor: 5.948

4.  Nested PCR method for rapid and sensitive detection of Vibrio vulnificus in fish, sediments, and water.

Authors:  C R Arias; E Garay; R Aznar
Journal:  Appl Environ Microbiol       Date:  1995-09       Impact factor: 4.792

5.  Development of a specific biotinylated DNA probe for the detection of Renibacterium salmoninarum.

Authors:  H Hariharan; B Qian; B Despres; F S Kibenge; S B Heaney; D J Rainnie
Journal:  Can J Vet Res       Date:  1995-10       Impact factor: 1.310

Review 6.  Taxonomy of bacterial fish pathogens.

Authors:  Brian Austin
Journal:  Vet Res       Date:  2011-02-02       Impact factor: 3.683

Review 7.  Applications of DNA amplification techniques in veterinary diagnostics.

Authors:  M Pfeffer; M Wiedmann; C A Batt
Journal:  Vet Res Commun       Date:  1995       Impact factor: 2.459
  7 in total

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