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Literature DB >> 7574657

Nested PCR method for rapid and sensitive detection of Vibrio vulnificus in fish, sediments, and water.

Abstract

A nested PCR for the detection of Vibrio vulnificus in fish farms was developed as an alternative to cultural methods by using universal primers flanking the V. vulnificus-specific sequences directed against 23S rRNA genes. This specific assay detected 10 fg of DNA or 12 to 120 cells in artificially inoculated samples without enrichment and within 24 h.

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Year: 1995 PMID： 7574657 PMCID： PMC167627 DOI： 10.1128/aem.61.9.3476-3478.1995

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

12 in total

1. Polymerase chain reaction identification of Vibrio vulnificus in artificially contaminated oysters.

Authors: W E Hill; S P Keasler; M W Trucksess; P Feng; C A Kaysner; K A Lampel
Journal: Appl Environ Microbiol Date: 1991-03 Impact factor: 4.792

2. Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli.

Authors: J Brosius; T J Dull; D D Sleeter; H F Noller
Journal: J Mol Biol Date: 1981-05-15 Impact factor: 5.469

3. Use of the polymerase chain reaction in detection of culturable and nonculturable Vibrio vulnificus cells.

Authors: L A Brauns; M C Hudson; J D Oliver
Journal: Appl Environ Microbiol Date: 1991-09 Impact factor: 4.792

4. Formation of nonculturable Vibrio vulnificus cells and its relationship to the starvation state.

Authors: J D Oliver; L Nilsson; S Kjelleberg
Journal: Appl Environ Microbiol Date: 1991-09 Impact factor: 4.792

5. Detection of Aeromonas salmonicida from fish by using polymerase chain reaction amplification of the virulence surface array protein gene.

Authors: C E Gustafson; C J Thomas; T J Trust
Journal: Appl Environ Microbiol Date: 1992-12 Impact factor: 4.792

6. Rapid polymerase chain reaction method for detection of Vibrio cholerae in foods.

Authors: W H Koch; W L Payne; B A Wentz; T A Cebula
Journal: Appl Environ Microbiol Date: 1993-02 Impact factor: 4.792

7. Rapid identification of Vibrio vulnificus on nonselective media with an alkaline phosphatase-labeled oligonucleotide probe.

Authors: A C Wright; G A Miceli; W L Landry; J B Christy; W D Watkins; J G Morris
Journal: Appl Environ Microbiol Date: 1993-02 Impact factor: 4.792

8. The risk of Vibrio illness in the Florida raw oyster eating population, 1981-1988.

Authors: J C Desenclos; K C Klontz; L E Wolfe; S Hoecherl
Journal: Am J Epidemiol Date: 1991-08-01 Impact factor: 4.897

9. A PCR-based assay for the identification of the fish pathogen Renibacterium salmoninarum.

Authors: G León; N Maulén; J Figueroa; J Villanueva; C Rodríguez; M I Vera; M Krauskopf
Journal: FEMS Microbiol Lett Date: 1994-01-15 Impact factor: 2.742

10. Detection of Aeromonas salmonicida, causal agent of furunculosis in salmonid fish, from the tank effluent of hatchery-reared Atlantic salmon smolts.

Authors: D O'Brien; J Mooney; D Ryan; E Powell; M Hiney; P R Smith; R Powell
Journal: Appl Environ Microbiol Date: 1994-10 Impact factor: 4.792

15 in total

1. A selective medium and a specific probe for detection of Vibrio vulnificus.

Authors: M Cerdà-Cuéllar; J Jofre; A R Blanch
Journal: Appl Environ Microbiol Date: 2000-02 Impact factor: 4.792

Review 2. Biodiversity of vibrios.

Authors: Fabiano L Thompson; Tetsuya Iida; Jean Swings
Journal: Microbiol Mol Biol Rev Date: 2004-09 Impact factor: 11.056

3. Detection of cytotoxin-hemolysin mRNA in nonculturable populations of environmental and clinical Vibrio vulnificus strains in artificial seawater.

Authors: Marion Fischer-Le Saux; Dominique Hervio-Heath; Solen Loaec; Rita R Colwell; Monique Pommepuy
Journal: Appl Environ Microbiol Date: 2002-11 Impact factor: 4.792

4. Direct identification of Vibrio vulnificus in clinical specimens by nested PCR.

Authors: S E Lee; S Y Kim; S J Kim; H S Kim; J H Shin; S H Choi; S S Chung; J H Rhee
Journal: J Clin Microbiol Date: 1998-10 Impact factor: 5.948

5. Deep-sea hydrothermal vent bacteria related to human pathogenic Vibrio species.

Authors: Nur A Hasan; Christopher J Grim; Erin K Lipp; Irma N G Rivera; Jongsik Chun; Bradd J Haley; Elisa Taviani; Seon Young Choi; Mozammel Hoq; A Christine Munk; Thomas S Brettin; David Bruce; Jean F Challacombe; J Chris Detter; Cliff S Han; Jonathan A Eisen; Anwar Huq; Rita R Colwell
Journal: Proc Natl Acad Sci U S A Date: 2015-05-11 Impact factor: 11.205