Literature DB >> 8899978

A simplified PCR-based method for the detection of Renibacterium salmoninarum utilizing preparations of rainbow trout (Oncorhynchus mykiss, Walbaum) lymphocytes.

D McIntosh1, P G Meaden, B Austin.   

Abstract

A method for the detection of Renibacterium salmoninarum by PCR is described. A rapid, reliable procedure was developed for the extraction of DNA, which could be applied to infected kidney homogenates and head kidney lymphocyte preparations. The target for DNA amplification was a 376-bp region of the gene encoding the 57-kDa major surface antigen (MSA). The PCR was specific for R. salmoninarum and allowed the detection of 10 to 100 cells of the pathogen. Use of the PCR for the examination of experimentally infected rainbow trout showed it to be as reliable as plate culture methods for the detection of R. salmoninarum in infected kidneys.

Entities:  

Mesh：

Substances：

Year:  1996        PMID： 8899978      PMCID： PMC168209          DOI： 10.1128/aem.62.11.3929-3932.1996

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  3 in total

1.  Molecular cloning and sequence analysis of the gene coding for the 57-kDa major soluble antigen of the salmonid fish pathogen Renibacterium salmoninarum.

Authors:  M S Chien; T L Gilbert; C Huang; M L Landolt; P J O'Hara; J R Winton
Journal:  FEMS Microbiol Lett       Date:  1992-09-15       Impact factor: 2.742

2.  A PCR-based assay for the identification of the fish pathogen Renibacterium salmoninarum.

Authors:  G León; N Maulén; J Figueroa; J Villanueva; C Rodríguez; M I Vera; M Krauskopf
Journal:  FEMS Microbiol Lett       Date:  1994-01-15       Impact factor: 2.742

3.  Renibacterium salmoninarum, the causative agent of bacterial kidney disease in salmonid fish, detected by nested reverse transcription-PCR of 16S rRNA sequences.

Authors:  H B Magnússon; O H Fridjónsson; O S Andrésson; E Benediktsdóttir; S Gudmundsdóttir; V Andrésdóttir
Journal:  Appl Environ Microbiol       Date:  1994-12       Impact factor: 4.792
  3 in total
  4 in total

1.  The gills are an important site of iNOS expression in rainbow trout Oncorhynchus mykiss after challenge with the gram-positive pathogen Renibacterium salmoninarum.

Authors:  J J Campos-Perez; M Ward; P S Grabowski; A E Ellis; C J Secombes
Journal:  Immunology       Date:  2000-01       Impact factor: 7.397

2.  A sensitive nested reverse transcriptase PCR assay to detect viable cells of the fish pathogen Renibacterium salmoninarum in Atlantic salmon (Salmo salar L.).

Authors:  M Cook; W H Lynch
Journal:  Appl Environ Microbiol       Date:  1999-07       Impact factor: 4.792

3.  Development of a PCR assay for detection of Yersinia ruckeri in tissues of inoculated and naturally infected trout.

Authors:  A Gibello; M M Blanco; M A Moreno; M T Cutuli; A Domenech; L Domínguez; J F Fernández-Garayzábal
Journal:  Appl Environ Microbiol       Date:  1999-01       Impact factor: 4.792

4.  Comparative Genomic Analysis of Two Chilean Renibacterium salmoninarum Isolates and the Type Strain ATCC 33209T.

Authors:  Jorn Bethke; Alejandro J Yáñez; Ruben Avendaño-Herrera
Journal:  Genome Biol Evol       Date:  2018-07-01       Impact factor: 3.416
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.