An unusual metal-binding cluster found exclusively in the avian breast muscle troponin T of Galliformes and Craciformes.

A repeating metal-binding (Cu2+ > Ni2+ > Zn2+ approximately Co2+) sequence (HE/AEAH)4 has been identified in troponin T isoforms specifically expressed in the breast but not leg muscles of all Galliformes and Craciformes. It is absent in the skeletal and cardiac muscles of mammals and all other avian species investigated. Concentration of the metal-binding sites is adequate to affect free metal levels in the muscle cell and we suggest a possible link between its presence in breast muscle of Galliformes and the high ratio of breast muscle to total body muscle mass and explosive but short-lived flight pattern of these birds. This sequence can be used for a highly selective metal-affinity chromatographic purification of muscle or engineered TnTs even in high salt and/or urea.