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Literature DB >> 8126172

Identification and detection of Trypanosoma cruzi by using a DNA amplification fingerprint obtained from the ribosomal intergenic spacer.

N González¹, I Galindo, P Guevara, E Novak, J V Scorza, N Añez, J F Da Silveira, J L Ramírez.

Abstract

We designed a PCR assay targeted on repeated elements of the ribosomal intergenic spacer which produces highly polymorphic DNA band patterns for different strains of Trypanosoma cruzi. By labeling the PCR products with digoxigenin and by chemiluminescence detection, we improved the assay sensitivity by three orders of magnitude to get T. cruzi strain fingerprints in feces of the trypanosome-infected triatomine bug vector. We also developed a capture assay for the digoxigenin-labeled PCR products that allowed us to detect T. cruzi in triatomine bug vector feces and in human serum samples with a solid support.

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Year: 1994 PMID： 8126172 PMCID： PMC262987 DOI： 10.1128/jcm.32.1.153-158.1994

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

22 in total

1. Diagnosis of Chagas disease using recombinant DNA technology.

Authors: A C Frasch; M B Reyes
Journal: Parasitol Today Date: 1990-04

2. Random amplified polymorphic DNA.

Authors: J M Macpherson; A A Gajadhar
Journal: Parasitol Today Date: 1992-07

3. Direct analysis of polymerase chain reaction products using enzyme-linked immunosorbent assay techniques.

Authors: A Landgraf; B Reckmann; A Pingoud
Journal: Anal Biochem Date: 1991-10 Impact factor: 3.365

4. Mathematical model for studying genetic variation in terms of restriction endonucleases.

Authors: M Nei; W H Li
Journal: Proc Natl Acad Sci U S A Date: 1979-10 Impact factor: 11.205

5. A combined polymerase chain reaction-colour development hybridization assay in a microtitre format for the detection of Clostridium spp.

Authors: I Galindo; R Rangel-Aldao; J L Ramírez
Journal: Appl Microbiol Biotechnol Date: 1993-07 Impact factor: 4.813

6. Schizodeme analysis of Trypanosoma cruzi stocks from South and Central America by analysis of PCR-amplified minicircle variable region sequences.

Authors: H Avila; A M Goncalves; N S Nehme; C M Morel; L Simpson
Journal: Mol Biochem Parasitol Date: 1990 Sep-Oct Impact factor: 1.759

7. Sensitive detection and schizodeme classification of Trypanosoma cruzi cells by amplification of kinetoplast minicircle DNA sequences: use in diagnosis of Chagas' disease.

Authors: N R Sturm; W Degrave; C Morel; L Simpson
Journal: Mol Biochem Parasitol Date: 1989-03-15 Impact factor: 1.759

8. Minichromosomal repetitive DNA in Trypanosoma cruzi: its use in a high-sensitivity parasite detection assay.

Authors: A Gonzalez; E Prediger; M E Huecas; N Nogueira; P M Lizardi
Journal: Proc Natl Acad Sci U S A Date: 1984-06 Impact factor: 11.205

9. Genetic characterization of six parasitic protozoa: parity between random-primer DNA typing and multilocus enzyme electrophoresis.

Authors: M Tibayrenc; K Neubauer; C Barnabé; F Guerrini; D Skarecky; F J Ayala
Journal: Proc Natl Acad Sci U S A Date: 1993-02-15 Impact factor: 11.205

4. Development and Application of an ELISA Assay Using Excretion/Secretion Proteins from Epimastigote Forms of T. cruzi (ESEA Antigens) for the Diagnosis of Chagas Disease.

Authors: Mariolga Berrizbeitia; Milagros Figueroa; Brian J Ward; Jessicca Rodríguez; Alicia Jorquera; Maria A Figuera; Leomerys Romero; Momar Ndao
Journal: J Trop Med Date: 2012-09-25

4 in total