Literature DB >> 7763926

A combined polymerase chain reaction-colour development hybridization assay in a microtitre format for the detection of Clostridium spp.

I Galindo1, R Rangel-Aldao, J L Ramírez.   

Abstract

We have developed a rapid and sensitive assay for the detection of clostridial cells or spores in liquid food samples. The method recognizes Clostridium 16S (small ribosomal subunit RNA) rDNA (ribosomal DNA) sequences by a polymerase chain reaction-digoxigenin-labelling protocol, coupled to a capture oligonucleotide immobilized on a microtitre plate. The positive results are revealed by means of a colour reaction. In 6 h of non-intensive labour, we can detect as few as two to five clostridial cells or spores in experimentally contaminated soft drinks.

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Year:  1993        PMID： 7763926     DOI： 10.1007/BF00205050

Source DB:  PubMed          Journal:  Appl Microbiol Biotechnol        ISSN： 0175-7598            Impact factor:   4.813


  10 in total

1.  Direct analysis of polymerase chain reaction products using enzyme-linked immunosorbent assay techniques.

Authors:  A Landgraf; B Reckmann; A Pingoud
Journal:  Anal Biochem       Date:  1991-10       Impact factor: 3.365

2.  Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses.

Authors:  D J Lane; B Pace; G J Olsen; D A Stahl; M L Sogin; N R Pace
Journal:  Proc Natl Acad Sci U S A       Date:  1985-10       Impact factor: 11.205

3.  Microplate hybridization of amplified viral DNA segment.

Authors:  S Inouye; R Hondo
Journal:  J Clin Microbiol       Date:  1990-06       Impact factor: 5.948

4.  Genome organization of the anaerobic pathogen Clostridium perfringens.

Authors:  B Canard; S T Cole
Journal:  Proc Natl Acad Sci U S A       Date:  1989-09       Impact factor: 11.205

5.  A procedure for productive coupling of synthetic oligonucleotides to polystyrene microtiter wells for hybridization capture.

Authors:  J A Running; M S Urdea
Journal:  Biotechniques       Date:  1990-03       Impact factor: 1.993

6.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors:  R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal:  Science       Date:  1988-01-29       Impact factor: 47.728

Review 7.  Molecular phylogeny of the animal kingdom.

Authors:  K G Field; G J Olsen; D J Lane; S J Giovannoni; M T Ghiselin; E C Raff; N R Pace; R A Raff
Journal:  Science       Date:  1988-02-12       Impact factor: 47.728

8.  Specific detection of Clostridium botulinum type B by using the polymerase chain reaction.

Authors:  E A Szabo; J M Pemberton; P M Desmarchelier
Journal:  Appl Environ Microbiol       Date:  1992-01       Impact factor: 4.792

9.  Genetic analysis of amplified DNA with immobilized sequence-specific oligonucleotide probes.

Authors:  R K Saiki; P S Walsh; C H Levenson; H A Erlich
Journal:  Proc Natl Acad Sci U S A       Date:  1989-08       Impact factor: 11.205

10.  Identification of toxigenic Clostridium difficile by the polymerase chain reaction.

Authors:  N Kato; C Y Ou; H Kato; S L Bartley; V K Brown; V R Dowell; K Ueno
Journal:  J Clin Microbiol       Date:  1991-01       Impact factor: 5.948
  10 in total
  4 in total

1.  A rapid PCR-based DNA test for enterotoxic Bacillus cereus.

Authors:  V Mäntynen; K Lindström
Journal:  Appl Environ Microbiol       Date:  1998-05       Impact factor: 4.792

2.  Identification and detection of Trypanosoma cruzi by using a DNA amplification fingerprint obtained from the ribosomal intergenic spacer.

Authors:  N González; I Galindo; P Guevara; E Novak; J V Scorza; N Añez; J F Da Silveira; J L Ramírez
Journal:  J Clin Microbiol       Date:  1994-01       Impact factor: 5.948

3.  Combined PCR-oligonucleotide ligation assay for rapid detection of Salmonella serovars.

Authors:  G G Stone; R D Oberst; M P Hays; S McVey; M M Chengappa
Journal:  J Clin Microbiol       Date:  1995-11       Impact factor: 5.948

4.  Presence of Leishmania braziliensis in blood samples from cured patients or at different stages of immunotherapy.

Authors:  P Guevara; E Rojas; N Gonzalez; J V Scorza; N Añez; M Valera; J L Ramírez
Journal:  Clin Diagn Lab Immunol       Date:  1994-07
  4 in total

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