Bovine microvascular endothelial cells of separate morphology differ in growth and response to the action of interferon-gamma.

Five cell types recently isolated from the bovine corpus luteum differed in their epithelioid morphology and their cytoskeleton, but shared common criteria of microvascular endothelial cells. To give strong evidence for the separate entity, the growth rate of the 5 phenotypically different cells was studied. They were seeded at low density on day 0. Most of these cells were treated with 200 to 1000 U recombinant bovine interferon-gamma (IFN-gamma) for 3 days. The untreated remainder served as controls. Cell counts were made for all cultures on days 4, 7, 10 and 13. Morphology: 13 d after treatment with IFN-gamma senescent cells as well as intact cells occurred in cultures of cell types 1 to 4. Cultures of cell type 5 were apparently unchanged and resembled their untreated counterparts. Desmin-positive cells in cultures of cell type 2 developed cell processes. Growth rate: In the absence of IFN-gamma, the growth rate was high for cell types 3 and 4, moderate for cell type 1, and low for cell types 2 and 5. The presence of IFN-gamma caused anti-proliferative effects. These were higher for cell types 3 and 4 than for cell types 1 and 2. IFN-gamma could be cytotoxic on cell type 3. In contrast, the cytokine tended to support the cell growth of cell type 5. These findings substantiate the postulate that endothelial cells exhibiting separate morphology in culture also function differently.