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Literature DB >> 810659

Regulated in vitro synthesis of the enzymes of the deo operon of Escerichia coli. properties of the DNA directed system.

Abstract

The four enzymes deoxyriboaldolase, thymidine phosporylase, deoxyribomutase, and purine nucleoside phosphorylase have been synthesized in substantial amounts in a DNA-dependent in vitro system programmed with DNA containing the deo operon. The synthesis is greatly stimulated by deoxyribose-5-phosphate and cyclic AMP indicating that the deoR repressor and the catabolite activating protein (CAP) are highly active under our cell-free conditions. In contrast it has not yet been possible to observe a reproducible effect of the cytR repressor in vitro. The sequential appearance of active enzymes has confirmed the direction of transcription as being dra-tpp-drm-pup and has indicated that the four genes are transcribed into a single tetracistronic message.

Entities: Chemical Gene

Mesh：

Substances：

Year: 1975 PMID： 810659 DOI： 10.1007/BF00703255

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

21 in total

1. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

Authors: K BURTON
Journal: Biochem J Date: 1956-02 Impact factor: 3.857

2. Relief of polarity in DNA-dependent cell-free synthesis of enzymes of the galactose operon of Escherichia coli.

Authors: W Wetekam; K Staack; R Ehring
Journal: Mol Gen Genet Date: 1972

3. Mutants constitutive for nucleoside-catabolizing enzymes in Escherichia coli K12. Isolation, charactrization and mapping.

Authors: A Munch-Petersen; P Nygaard; K Hammer-Jespersen; N Fiil
Journal: Eur J Biochem Date: 1972-05-23

4. Regulated in vitro synthesis of Escherichia coli tryptophan operon messenger ribonucleic acid and enzymes.

Authors: H Zalkin; C Yanofsky; C L Squires
Journal: J Biol Chem Date: 1974-01-25 Impact factor: 5.157

5. An adenosine 3':5'-cyclic monophosphate-binding protein that acts on the transcription process.

Authors: L Eron; R Arditti; G Zubay; S Connaway; J R Beckwith
Journal: Proc Natl Acad Sci U S A Date: 1971-01 Impact factor: 11.205

6. A regulatory mutant affecting the synthesis of enzymes involved in the catabolism of nucleosides in Escherichia coli.

Authors: S I Ahmad; R H Pritchard
Journal: Mol Gen Genet Date: 1971

7. A map of four genes specifying enzymes involved in catabolism of nucleosides and deoxynucleosides in Escherichia coli.

Authors: S I Ahmad; R H Pritchard
Journal: Mol Gen Genet Date: 1969-08-15

8. The absence of deoxyriboaldolase activity in a thymineless mutant of Escherichia coli strain 15: a possible explanation for the low thymine requirement of some thymineless strains.

Authors: T R Breitman; R M Bradford
Journal: Biochim Biophys Acta Date: 1967-03-29

9. On the catabolism of deoxyribonucleosides in cells and cell extracts of Escherichia coli.

Authors: A Munch-Petersen
Journal: Eur J Biochem Date: 1968-11

10. Cytidine deaminase from Escherichia coli. Purification, properties and inhibition by the potential transition state analog 3,4,5,6-tetrahydrouridine.

Authors: R M Cohen; R Wolfenden
Journal: J Biol Chem Date: 1971-12-25 Impact factor: 5.157

12 in total

1. Multiple regulation of nucleoside catabolizing enzymes: regulation of the deo operon by the cytR and deoR gene products.

Authors: K Hammer-Jespersen; A Munch-Ptersen
Journal: Mol Gen Genet Date: 1975

2. On the structure of the deo operon of Escherichia coli.

Authors: P Jorgensen; J Collins; P Valentin-Hansen
Journal: Mol Gen Genet Date: 1977-09-21

3. Regulation of the deo operon in Escherichia coli: the double negative control of the deo operon by the cytR and deoR repressors in a DNA directed in vitro system.

Authors: P Valentin-Hansen; B A Svenningsen; A Munch-Petersen; K Hammer-Jespersen
Journal: Mol Gen Genet Date: 1978-02-16

Review 4. Recalibrated linkage map of Escherichia coli K-12.

Authors: B J Bachmann; K B Low; A L Taylor
Journal: Bacteriol Rev Date: 1976-03

5. The primary structure of the DeoR repressor from Escherichia coli K-12.

Authors: P Valentin-Hansen; P Højrup; S Short
Journal: Nucleic Acids Res Date: 1985-08-26 Impact factor: 16.971

6. Reevaluation of the mode of action of streptolydigin in Escherichia coli: induction of transcription termination in vivo.

Authors: K von Meyenburg; L D Nielsen; K Johnsen; S Molin; B Svenningsen; G Miozzari
Journal: Antimicrob Agents Chemother Date: 1978-02 Impact factor: 5.191

10. Insertion of bacteriophage lambda into the deo operon of Escherichia coli K-12 and isolation of plaque-forming lambdadeo+ transducing bacteriophages.

Authors: R S Buxton; K Hammer-Jespersen; T D Hansen
Journal: J Bacteriol Date: 1978-11 Impact factor: 3.490