Literature DB >> 8080082

Determination of lipase activity by a rhodamine-triglyceride-agarose assay.

J F Jette1, E Ziomek.   

Abstract

A quantitative fluorescence lipase assay based on the interaction of rhodamine B with fatty acids released during the enzymatic hydrolysis of triglycerides is described. The assay is linear over the range of 0.5-2 mM oleic acid and 0.05-1 microgram pure lipase. The method allows flexibility in the choice of substrate. A large number of samples can be assayed simultaneously, making it practical for assaying lipase activity in column fractions during purification. The substrate profiles of Geotrichum candidum lipase obtained by both a titrimetric assay and the RTA assay indicated the highest activity against triolein. The method is rapid and can be further automated.

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Year:  1994        PMID： 8080082     DOI： 10.1006/abio.1994.1265

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  9 in total

1.  Purification and characterization of a Penicillium sp. lipase which discriminates against diglycerides.

Authors:  K Gulomova; E Ziomek; J D Schrag; K Davranov; M Cygler
Journal:  Lipids       Date:  1996-04       Impact factor: 1.880

2.  Identification and Characterization of a Novel Thermophilic, Organic Solvent Stable Lipase of Bacillus from a Hot Spring.

Authors:  Jiang Li; Xiumeng Liu
Journal:  Lipids       Date:  2017-06-03       Impact factor: 1.880

3.  Isolation of a novel lipase gene from Serratia liquefaciens S33 DB-1, functional expression in Pichia pastoris and its properties.

Authors:  Hongyan Yao; Shunwu Yu; Lida Zhang; Kaijing Zuo; Hua Ling; Fei Zhang; Kexuan Tang
Journal:  Mol Biotechnol       Date:  2007-10-10       Impact factor: 2.695

Review 4.  Halophilic archaea and their potential to generate renewable fuels and chemicals.

Authors:  Lakshmi Kasirajan; Julie A Maupin-Furlow
Journal:  Biotechnol Bioeng       Date:  2020-12-16       Impact factor: 4.530

5.  Enzyme engineering: A synthetic biology approach for more effective library generation and automated high-throughput screening.

Authors:  Daniela Quaglia; Maximilian C C J C Ebert; Paul F Mugford; Joelle N Pelletier
Journal:  PLoS One       Date:  2017-02-08       Impact factor: 3.240

6.  Zymography for Picogram Detection of Lipase and Esterase Activities.

Authors:  Andre Mong Jie Ng; Hongfang Zhang; Giang Kien Truc Nguyen
Journal:  Molecules       Date:  2021-03-11       Impact factor: 4.411

7.  Diversity and bioprospecting for industrial hydrolytic enzymes of microbial communities isolated from deserted areas of south-east Morocco.

Authors:  Amina Manni; Abdelkarim Filali-Maltouf
Journal:  AIMS Microbiol       Date:  2022-01-20

8.  Bacillus cereus (EG-Q3) in the Gut of Ectropis grisescens Contributes to Host Response to Starvation Conditions.

Authors:  Xiayu Li; Yong Zhang; Linlin Zhou; Tian Gao; Yu Zhao; Song Liu; Qingqing Su; Chaoling Wei; Yunqiu Yang; Yanhua Long
Journal:  Front Microbiol       Date:  2022-04-11       Impact factor: 6.064

9.  Phytochemical and antimicrobial investigation of the leaves of five Egyptian mango cultivars and evaluation of their essential oils as preservatives materials.

Authors:  Salama A Ouf; Alaaeldin M F Galal; Heba S Ibrahim; Amal Z Hassan; Maged K G Mekhael; Khaled F El-Yasergy; Mohamed N Abd El-Ghany; Mohamed A Rizk; Atef G Hanna
Journal:  J Food Sci Technol       Date:  2020-10-01       Impact factor: 3.117
  9 in total

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