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Literature DB >> 8743049

Purification and characterization of a Penicillium sp. lipase which discriminates against diglycerides.

K Gulomova¹, E Ziomek, J D Schrag, K Davranov, M Cygler.

Abstract

A lipase was isolated from Penicillium sp. strain UZLM-4 and characterized. This lipase has a molecular weight of 27,344 (determined by mass spectrometry) and hydrolyzes triglycerides in preference to mono- and diglyceride substrates. Among various triglyceride substrates, tributyrin is hydrolyzed about four times faster than any other tested. The lipase has a preference for hydrolysis at the 1,3 positions of the lipids and shows a weak stereoselectivity for the S enantiomer. Unlike most other lipases, this lipase is stable and has a high activity at low surface pressures (5-10 mN/m).

Entities: Chemical Species

Mesh：

Substances：

Year: 1996 PMID： 8743049 DOI： 10.1007/bf02522923

Source DB: PubMed Journal: Lipids ISSN： 0024-4201 Impact factor: 1.880

27 in total

1. Assay of proteins in the presence of interfering materials.

Authors: A Bensadoun; D Weinstein
Journal: Anal Biochem Date: 1976-01 Impact factor: 3.365

2. Geotrichum candidum produces several lipases with markedly different substrate specificities.

Authors: C M Sidebottom; E Charton; P P Dunn; G Mycock; C Davies; J L Sutton; A R Macrae; A R Slabas
Journal: Eur J Biochem Date: 1991-12-05

3. Cloning and structure of the mono- and diacylglycerol lipase-encoding gene from Penicillium camembertii U-150.

Authors: S Yamaguchi; T Mase; K Takeuchi
Journal: Gene Date: 1991-07-15 Impact factor: 3.688

Review 4. Enzymes in the synthesis of chiral drugs.

Authors: A L Margolin
Journal: Enzyme Microb Technol Date: 1993-04 Impact factor: 3.493

5. A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex.

Authors: A M Brzozowski; U Derewenda; Z S Derewenda; G G Dodson; D M Lawson; J P Turkenburg; F Bjorkling; B Huge-Jensen; S A Patkar; L Thim
Journal: Nature Date: 1991-06-06 Impact factor: 49.962

6. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.

Authors: P Matsudaira
Journal: J Biol Chem Date: 1987-07-25 Impact factor: 5.157

7. The sequence, crystal structure determination and refinement of two crystal forms of lipase B from Candida antarctica.

Authors: J Uppenberg; M T Hansen; S Patkar; T A Jones
Journal: Structure Date: 1994-04-15 Impact factor: 5.006

Purification and characterization of a Penicillium sp. lipase which discriminates against diglycerides.

1. Assay of proteins in the presence of interfering materials.

2. Geotrichum candidum produces several lipases with markedly different substrate specificities.

3. Cloning and structure of the mono- and diacylglycerol lipase-encoding gene from Penicillium camembertii U-150.

Review 4. Enzymes in the synthesis of chiral drugs.

5. A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex.

6. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.

7. The sequence, crystal structure determination and refinement of two crystal forms of lipase B from Candida antarctica.

8. Current progress in crystallographic studies of new lipases from filamentous fungi.

9. Stereoselective hydrolysis of triglycerides by animal and microbial lipases.

Review 10. Application of enzymes to the synthesis of surfactants.

1. Biochemical and structural characterization of triacylglycerol lipase from Penicillium cyclopium.

2. Kinetic properties of Penicillium cyclopium lipases studied with vinyl esters.