| Literature DB >> 33799781 |
Andre Mong Jie Ng1,2,3, Hongfang Zhang3, Giang Kien Truc Nguyen3.
Abstract
Lipases and esterases are important catalysts with wide varieties of industrial applications. Although many methods have been established for detecting their activities, a simple and sensitive approach for picogram detection of lipolytic enzyme quantity is still highly desirable. Here we report a lipase detection assay which is 1000-fold more sensitive than previously reported methods. Our assay enables the detection of as low as 5 pg and 180 pg of lipolytic activity by direct spotting and zymography, respectively. Furthermore, we demonstrated that the detection sensitivity was adjustable by varying the buffering capacity, which allows for screening of both high and low abundance lipolytic enzymes. Coupled with liquid chromatography-mass spectrometry, our method provides a useful tool for sensitive detection and identification of lipolytic enzymes.Entities:
Keywords: esterase; lipase; picogram; zymogram
Year: 2021 PMID: 33799781 PMCID: PMC7998489 DOI: 10.3390/molecules26061542
Source DB: PubMed Journal: Molecules ISSN: 1420-3049 Impact factor: 4.411