Literature DB >> 8051251

Direct detection of Vibrio cholerae in stool samples.

P Varela1, G D Pollevick, M Rivas, I Chinen, N Binsztein, A C Frasch, R A Ugalde.   

Abstract

A direct method to detect Vibrio cholerae in stool samples was developed by using a PCR procedure that did not require a DNA purification step. Dilution (1/100) of stool samples prevented inhibition of the reaction by contaminants, and two consecutive PCRs, the second one with a nested primer, achieved the desired sensitivity. Comparison of the results obtained from stool swab samples processed by the two-step PCR and by an enzyme-linked immunosorbent assay using GM1 as the capture molecule showed that the former is more sensitive and gave positive results even when V. cholerae was not culturable or dead.

Entities:  

Mesh：

Substances：

Year:  1994        PMID： 8051251      PMCID： PMC263658          DOI： 10.1128/jcm.32.5.1246-1248.1994

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  13 in total

1.  Cholera toxin genes: nucleotide sequence, deletion analysis and vaccine development.

Authors:  J J Mekalanos; D J Swartz; G D Pearson; N Harford; F Groyne; M de Wilde
Journal:  Nature       Date:  1983 Dec 8-14       Impact factor: 49.962

2.  Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reactions.

Authors:  J Wilde; J Eiden; R Yolken
Journal:  J Clin Microbiol       Date:  1990-06       Impact factor: 5.948

3.  Molecular characterization of environmental and nontoxigenic strains of Vibrio cholerae.

Authors:  J B Kaper; S L Moseley; S Falkow
Journal:  Infect Immun       Date:  1981-05       Impact factor: 3.441

4.  Rapid polymerase chain reaction method for detection of Vibrio cholerae in foods.

Authors:  W H Koch; W L Payne; B A Wentz; T A Cebula
Journal:  Appl Environ Microbiol       Date:  1993-02       Impact factor: 4.792

5.  Polymerase chain reaction for detection of the cholera enterotoxin operon of Vibrio cholerae.

Authors:  H Shirai; M Nishibuchi; T Ramamurthy; S K Bhattacharya; S C Pal; Y Takeda
Journal:  J Clin Microbiol       Date:  1991-11       Impact factor: 5.948

6.  Production of cholera-like enterotoxin by a Vibrio cholerae non-O1 strain isolated from the environment.

Authors:  J P Craig; K Yamamoto; Y Takeda; T Miwatani
Journal:  Infect Immun       Date:  1981-10       Impact factor: 3.441

7.  Identification of toxigenic Vibrio cholerae from the Argentine outbreak by PCR for ctx A1 and ctx A2-B.

Authors:  P Varela; M Rivas; N Binsztein; M L Cremona; P Herrmann; O Burrone; R A Ugalde; A C Frasch
Journal:  FEBS Lett       Date:  1993-01-02       Impact factor: 4.124

8.  Detection of toxigenic Clostridium difficile in stool specimens by the polymerase chain reaction.

Authors:  N Kato; C Y Ou; H Kato; S L Bartley; C C Luo; G E Killgore; K Ueno
Journal:  J Infect Dis       Date:  1993-02       Impact factor: 5.226

9.  Use of polymerase chain reaction for detection of toxigenic Vibrio cholerae O1 strains from the Latin American cholera epidemic.

Authors:  P I Fields; T Popovic; K Wachsmuth; O Olsvik
Journal:  J Clin Microbiol       Date:  1992-08       Impact factor: 5.948

10.  [Production of thermolabile exotoxin by strains of Escherichia coli isolated in Argentina].

Authors:  M Rivas; N Binsztein; L López Moral; R Cinto
Journal:  Rev Argent Microbiol       Date:  1987 Jul-Sep       Impact factor: 1.852
View more
  5 in total

1.  Viable but nonculturable Vibrio cholerae O1 in the aquatic environment of Argentina.

Authors:  Norma Binsztein; Marcela C Costagliola; Mariana Pichel; Verónica Jurquiza; Fernando C Ramírez; Rut Akselman; Marta Vacchino; Anwarul Huq; Rita Colwell
Journal:  Appl Environ Microbiol       Date:  2004-12       Impact factor: 4.792

2.  Genetic diversity of Vibrio cholerae O1 in Argentina and emergence of a new variant.

Authors:  Mariana Pichel; Marta Rivas; Isabel Chinen; Fernando Martín; Cristina Ibarra; Norma Binsztein
Journal:  J Clin Microbiol       Date:  2003-01       Impact factor: 5.948

3.  The liposome PCR assay is more sensitive than the Vibrio cholerae enterotoxin and Escherichia coli heat-labile enterotoxin reversed passive latex agglutination test at detecting cholera toxin in feces and water.

Authors:  David L Evers; Junkun He; Jeffrey T Mason; Timothy J O'Leary
Journal:  J Clin Microbiol       Date:  2010-10-20       Impact factor: 5.948

4.  Direct Dried Stool Sampling on Filter Paper for Molecular Analyses of Cholera.

Authors:  Stanislas Rebaudet; Sandra Moore; Anne-Cécile Normand; Lamine Koivogui; Eric Garnotel; Amara Jambai; Renaud Piarroux
Journal:  Am J Trop Med Hyg       Date:  2016-07-06       Impact factor: 2.345

5.  Genomics of the Argentinian cholera epidemic elucidate the contrasting dynamics of epidemic and endemic Vibrio cholerae.

Authors:  Matthew J Dorman; Daryl Domman; Tomás Poklepovich; Charlotte Tolley; Gisella Zolezzi; Leanne Kane; María Rosa Viñas; Marcela Panagópulo; Miriam Moroni; Norma Binsztein; María Inés Caffer; Simon Clare; Gordon Dougan; George P C Salmond; Julian Parkhill; Josefina Campos; Nicholas R Thomson
Journal:  Nat Commun       Date:  2020-10-01       Impact factor: 14.919
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.