Literature DB >> 20962142

The liposome PCR assay is more sensitive than the Vibrio cholerae enterotoxin and Escherichia coli heat-labile enterotoxin reversed passive latex agglutination test at detecting cholera toxin in feces and water.

David L Evers1, Junkun He, Jeffrey T Mason, Timothy J O'Leary.   

Abstract

Practical detection of cholera toxin (CT) by a liposome PCR (LPCR) immunoassay was compared to that of an established V. cholerae enterotoxin and Escherichia coli heat-labile enterotoxin reversed passive latex agglutination (VET-RPLA) assay. LPCR detected CT in the range of 10 pg/ml to 100 ng/ml in simulated feces and environmental water. Detection by VET-RPLA required at least 4 to 19 ng/ml CT.

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Year:  2010        PMID： 20962142      PMCID： PMC3008463          DOI： 10.1128/JCM.02019-10

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  12 in total

1.  Ultrasensitive enzymatic radioimmunoassay: application to detection of cholera toxin and rotavirus.

Authors:  C C Harris; R H Yolken; H Krokan; I C Hsu
Journal:  Proc Natl Acad Sci U S A       Date:  1979-10       Impact factor: 11.205

2.  A liposome-PCR assay for the ultrasensitive detection of biological toxins.

Authors:  Jeffrey T Mason; Lixin Xu; Zong-mei Sheng; Timothy J O'Leary
Journal:  Nat Biotechnol       Date:  2006-04-16       Impact factor: 54.908

3.  Liposome polymerase chain reaction assay for the sub-attomolar detection of cholera toxin and botulinum neurotoxin type A.

Authors:  Jeffrey T Mason; Lixin Xu; Zong-mei Sheng; Junkun He; Timothy J O'Leary
Journal:  Nat Protoc       Date:  2006       Impact factor: 13.491

4.  Improved purification process for cholera toxin and its application to the quantification of residual toxin in cholera vaccines.

Authors:  Hyun Jang; Hyo Seung Kim; Jeong Ah Kim; Jin Ho Seo; Rodney Carbis
Journal:  J Microbiol Biotechnol       Date:  2009-01       Impact factor: 2.351

5.  Comparative study of different methods for detection of toxic and other enzymatic factors in Vibrio cholerae strains.

Authors:  Anca Israil; Carmen Balotescu; Maria Damian; Cristina Dinu; Nadia Bucurenci
Journal:  Roum Arch Microbiol Immunol       Date:  2004 Jan-Jun

6.  Quantitative studies of toxin in the stools and jejunal aspirates of patients with cholera.

Authors:  K M Aziz; W H Mosley
Journal:  J Infect Dis       Date:  1972-01       Impact factor: 5.226

7.  Evaluation of the bead enzyme-linked immunosorbent assay for detection of cholera toxin directly from stool specimens.

Authors:  T Ramamurthy; S K Bhattacharya; Y Uesaka; K Horigome; M Paul; D Sen; S C Pal; T Takeda; Y Takeda; G B Nair
Journal:  J Clin Microbiol       Date:  1992-07       Impact factor: 5.948

Review 8.  Cholera.

Authors:  David A Sack; R Bradley Sack; G Balakrish Nair; A K Siddique
Journal:  Lancet       Date:  2004-01-17       Impact factor: 79.321

9.  Direct detection of Vibrio cholerae in stool samples.

Authors:  P Varela; G D Pollevick; M Rivas; I Chinen; N Binsztein; A C Frasch; R A Ugalde
Journal:  J Clin Microbiol       Date:  1994-05       Impact factor: 5.948

10.  Comparison of a reversed passive latex agglutination and a polymerase chain reaction for identification of cholera toxin producing Vibrio cholerae O1.

Authors:  Y Honma; N Higa; T Tsuji; M Iwanaga
Journal:  Microbiol Immunol       Date:  1995       Impact factor: 1.955
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  1 in total

1.  Quantitative detection of Vibrio cholera toxin by real-time and dynamic cytotoxicity monitoring.

Authors:  Dazhi Jin; Yun Luo; Min Zheng; Haijing Li; Jing Zhang; Melinda Stampfl; Xiao Xu; Gangqiang Ding; Yanjun Zhang; Yi-Wei Tang
Journal:  J Clin Microbiol       Date:  2013-09-18       Impact factor: 5.948
  1 in total

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