Literature DB >> 803973

Two improved methods for preparing ferritin-protein conjugates for electron microscopy.

Y Kishida, B R Olsen, R A Berg, D J Prockop.   

Abstract

Two improved procedures were developed for activating ferritin so that the ferritin could be covalently linked to antibodies. One procedure involved use of a water-soluble carbodiimide and N-hydroxysuccinimide to prepare ferritin-containing activated esters. The other involved activation of the ferritin with excess glutaraldehyde. The ferritin-antibody conjugates prepared with the two procedures were shown to have a number of properties which made them suitable for locating antigenic components in cells.

Entities:  

Mesh：

Substances：

Year:  1975        PMID： 803973      PMCID： PMC2109494          DOI： 10.1083/jcb.64.2.331

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  29 in total

1.  Characterization and purification of ferritin-antibody globulin conjugates.

Authors:  F BOREK; A M SILVERSTEIN
Journal:  J Immunol       Date:  1961-11       Impact factor: 5.422

2.  Preparation of an electron-dense antibody conjugate.

Authors:  S J SINGER
Journal:  Nature       Date:  1959-05-30       Impact factor: 49.962

3.  The hydrolysis of rabbit y-globulin and antibodies with crystalline papain.

Authors:  R R PORTER
Journal:  Biochem J       Date:  1959-09       Impact factor: 3.857

4.  Enzyme-labeled antibodies for the light and electron microscopic localization of tissue antigens.

Authors:  P K Nakane; G B Pierce
Journal:  J Cell Biol       Date:  1967-05       Impact factor: 10.539

5.  The use of bis-diazotized benzidine for preparing ferritin-conjugated antibodies for electron microscopy.

Authors:  M A Williams; D W Gregory
Journal:  J R Microsc Soc       Date:  1967-04

6.  Ultrastructural localization of contractile protein (thrombosthenin) in human platelets using an unlabeled antibody-peroxidase staining technique.

Authors:  F M Booyse; L A Sternberger; D Zschocke; M E Rafelson
Journal:  J Histochem Cytochem       Date:  1971-09       Impact factor: 2.479

7.  A general method for the specific staining of intracellular antigens with ferritin-antibody conjugates.

Authors:  J D McLean; S J Singer
Journal:  Proc Natl Acad Sci U S A       Date:  1970-01       Impact factor: 11.205

8.  Glutaraldehyde as a protein cross-linkage reagent.

Authors:  F M Richards; J R Knowles
Journal:  J Mol Biol       Date:  1968-10-14       Impact factor: 5.469

9.  Immunoferritin localization of intracellular antigens: the use of ultracryotomy to obtain ultrathin sections suitable for direct immunoferritin staining.

Authors:  R G Painter; K T Tokuyasu; S J Singer
Journal:  Proc Natl Acad Sci U S A       Date:  1973-06       Impact factor: 11.205

10.  Preparation of antibody-ferritin conjugates for immunoelectron microscopy.

Authors:  J SRI RAM; S S TAWDE; G B PIERCE; A R MIDGLEY
Journal:  J Cell Biol       Date:  1963-06       Impact factor: 10.539
View more
  56 in total

1.  Application of rapid freezing followed by freeze-substitution acrolein fixation for cytochemical studies of the rat stomach.

Authors:  F Murata; S Suzuki; S Tsuyama; T Suganuma; M Imada; C Furihata
Journal:  Histochem J       Date:  1985-09

2.  Three-dimensional electron microscopical visualization of the cytoskeleton of animal cells: immunoferritin identification of actin- and tubulin-containing structures.

Authors:  R E Webster; D Henderson; M Osborn; K Weber
Journal:  Proc Natl Acad Sci U S A       Date:  1978-11       Impact factor: 11.205

3.  Light and electron microscopic localization of L-alpha-hydroxyacid oxidase in rat kidney revealed by immunocytochemical techniques.

Authors:  S Yokota; K Ichikawa; T Hashimoto
Journal:  Histochemistry       Date:  1985

4.  Immunoelectron microscopic localization of penicillinase in Bacillus licheniformis.

Authors:  A Ghosh; B K Ghosh
Journal:  J Bacteriol       Date:  1979-03       Impact factor: 3.490

5.  Compartment ablation analysis of the insulin-responsive glucose transporter (GLUT4) in 3T3-L1 adipocytes.

Authors:  C Livingstone; D E James; J E Rice; D Hanpeter; G W Gould
Journal:  Biochem J       Date:  1996-04-15       Impact factor: 3.857

6.  Immunocytochemical localization of pepsinogen in rat stomach.

Authors:  S Suzuki; C Furihata; S Tsuyama; F Murata
Journal:  Histochemistry       Date:  1983

7.  Immuno-electron-microscopic studies on the subcellular distribution of rat liver epoxide hydrolase and the effect of phenobarbitone and 2-acetamidofluorene treatment.

Authors:  F Waechter; P Bentley; M Germann; F Oesch; W Stäubli
Journal:  Biochem J       Date:  1982-03-15       Impact factor: 3.857

8.  Cell surface-localized alkaline phosphatase of Escherichia coli as visualized by reaction product deposition and ferritin-labeled antibodies.

Authors:  T J MacaAlister; R T Irvin; J W Costerton
Journal:  J Bacteriol       Date:  1977-04       Impact factor: 3.490

9.  Immunocytochemical localization of alpha-actinin in intestinal epithelial cells.

Authors:  B Geiger; K T Tokuyasu; S J Singer
Journal:  Proc Natl Acad Sci U S A       Date:  1979-06       Impact factor: 11.205

10.  Quantitative immunocytochemical studies on differential induction of serine:pyruvate aminotransferase in mitochondria and peroxisomes of rat liver cells by administration of glucagon or di-(2-ethylhexyl)phthalate.

Authors:  S Yokota
Journal:  Histochemistry       Date:  1986
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.