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Literature DB >> 4189988

A general method for the specific staining of intracellular antigens with ferritin-antibody conjugates.

Abstract

A general method is described whereby an intracellular macromolecule can be specifically stained with its antibody conjugated to ferritin. In this method cells or cell organelles are fixed, embedded in bovine serum albumin cross-linked with glutaraldehyde or formaldehyde, and then sectioned. This procedure preserves antigenic determinants as well as the cellular ultrastructure. The application of the ferritin-antibody conjugate to the section produces specific staining of the exposed antigen.

Entities: Chemical Species

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Year: 1970 PMID： 4189988 PMCID： PMC286200 DOI： 10.1073/pnas.65.1.122

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

13 in total

1. FERRITIN-ANTIBODY CONJUGATES AS STAINS FOR ELECTRON MICROSCOPY.

Authors: S J SINGER; J D MCLEAN
Journal: Lab Invest Date: 1963-10 Impact factor: 5.662

2. The chemistry of the cell nucleus.

Authors: V G ALLFREY; A E MIRSKY; H STERN
Journal: Adv Enzymol Relat Subj Biochem Date: 1955

3. Electron microscopic localization of intracellular viral antigen by the use of ferritin-conjugated antibody.

Authors: C MORGAN; R A RIFKIND; K C HSU; M HOLDEN; B C SEEGAL; H M ROSE
Journal: Virology Date: 1961-06 Impact factor: 3.616

4. ON THE PRESERVATION OF ANTIGENIC DETERMINANTS DURING FIXATION AND EMBEDDING FOR ELECTRON MICROSCOPY.

Authors: R S Spendlove; S J Singer
Journal: Proc Natl Acad Sci U S A Date: 1961-01 Impact factor: 11.205

5. Post-embedding staining with ferritin labelled antibodies.

Authors: R O Thomson; P D Walker; I Batty; A Baillie
Journal: Nature Date: 1967-07-22 Impact factor: 49.962

6. CROSS-LINKED POLYAMPHOLYTES. NEW WATER-SOLUBLE EMBEDDING MEDIA FOR ELECTRON MICROSCOPY.

Authors: J D MCLEAN; S J SINGER
Journal: J Cell Biol Date: 1964-03 Impact factor: 10.539

7. Structure in nucleated erythrocytes.

Authors: H G DAVIES
Journal: J Biophys Biochem Cytol Date: 1961-03

8. Alteration of the conformation of proteins in red blood cell membranes and in solution by fixatives used in electron microscopy.

Authors: J Lenard; S J Singer
Journal: J Cell Biol Date: 1968-04 Impact factor: 10.539

9. Ultrathin frozen sections. I. Methods and ultrastructural preservation.

Authors: W Bernhard; E H Leduc
Journal: J Cell Biol Date: 1967-09 Impact factor: 10.539

10. Some enzymes of isolated nuclei.

Authors: H STERN; V ALLFREY; A E MIRSKY; H SAETREN
Journal: J Gen Physiol Date: 1952-01 Impact factor: 4.086

23 in total

1. Molecular immunolabeling with recombinant single-chain variable fragment (scFv) antibodies designed with metal-binding domains.

Authors: Marek Malecki; Annie Hsu; Lynn Truong; Sylvia Sanchez
Journal: Proc Natl Acad Sci U S A Date: 2001-12-26 Impact factor: 11.205

2. Detection of type A oncornavirus-like structures in chicken embryo cells after infection with Rous sarcoma virus.

Authors: G G Maul; L J Lewandowski
Journal: J Virol Date: 1975-10 Impact factor: 5.103

3. Immunocytochemical localization of soluble protein in the adrenal medulla.

Authors: D H Smith; J A Redick; D E Van Orden; L S Van Orden
Journal: Histochem J Date: 1973-05

4. Studies on mouse liver urate oxidase. 3. Fine localization of urate oxidase in liver cells revealed by means of ultracryotomy-immunoferritin method.

Authors: S Yokota; T Nagata
Journal: Histochemistry Date: 1974-05-03

5. Solid-phase conjugation of ferritin to Fab-fragments of immunoglobulin G for use in antigen localization on thin sections.

Authors: J P Kraehenbuhl; J D Jamieson
Journal: Proc Natl Acad Sci U S A Date: 1972-07 Impact factor: 11.205