Literature DB >> 7046735

Immuno-electron-microscopic studies on the subcellular distribution of rat liver epoxide hydrolase and the effect of phenobarbitone and 2-acetamidofluorene treatment.

F Waechter, P Bentley, M Germann, F Oesch, W Stäubli.   

Abstract

The distribution of rat liver epoxide hydrolase in various subcellular fractions was investigated by immuno-electron-microscopy. Ferritin-linked monospecific anti-(epoxide hydrolase) immunoglobulins bound specifically to the cytoplasmic surfaces of total microsomal preparations and smooth and rough microsomal fractions as well as the nuclear envelope. Specific binding was not observed when the ferritin conjugates were incubated with peroxisomes, lysosomes and mitochondria. The average specific ferritin load of the individual subcellular fractions correlated well with the measured epoxide hydrolase activities. This correlation was observed with fractions prepared from control, phenobarbitone-treated and 2-acetamidofluorene-treated rats.

Entities:  

Mesh：

Substances：

Year:  1982        PMID： 7046735      PMCID： PMC1158162          DOI： 10.1042/bj2020677

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  44 in total

Review 1.  Mammalian epoxide hydrases: inducible enzymes catalysing the inactivation of carcinogenic and cytotoxic metabolites derived from aromatic and olefinic compounds.

Authors:  F Oesch
Journal:  Xenobiotica       Date:  1973-05       Impact factor: 1.908

2.  A spin-label study of the role of phospholipids in the regulation of membrane-bound microsomal enzymes.

Authors:  S Eletr; D Zakim; D A Vessey
Journal:  J Mol Biol       Date:  1973-08-05       Impact factor: 5.469

3.  [Studies on the distribution of enzyme proteins in liver-cell endoplasmic membranes. I. Separation of rat liver microsomes by differential and zonal centrifugation].

Authors:  H U Schulze; H Staudinger
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1971-12

4.  A radiometric assay for hepatic epoxide hydrase activity with [7-3H] styrene oxide.

Authors:  E Oesch; D M Jerina; J Daly
Journal:  Biochim Biophys Acta       Date:  1971-03-10

5.  Induction, activation and inhibition of epoxide hydrase: an anomalous prevention of chlorobenzene-induced hepatotoxicity by an inhibitor of epoxide hydrase.

Authors:  F Oesch; D M Jerina; J W Daly; J M Rice
Journal:  Chem Biol Interact       Date:  1973-03       Impact factor: 5.192

6.  A low-viscosity epoxy resin embedding medium for electron microscopy.

Authors:  A R Spurr
Journal:  J Ultrastruct Res       Date:  1969-01

7.  Nonphosphorylating respiration of mitochondria from brown adipose tissue of rats.

Authors:  R E Smith; J C Roberts; K J Hittelman
Journal:  Science       Date:  1966-11-04       Impact factor: 47.728

8.  Electron microscopic examination of subcellular fractions. I. The preparation of representative samples from suspensions of particles.

Authors:  P Baudhuin; P Evrard; J Berthet
Journal:  J Cell Biol       Date:  1967-01       Impact factor: 10.539

9.  Correlated morphometric and biochemical studies on the liver cell. I. Morphometric model, stereologic methods, and normal morphometric data for rat liver.

Authors:  E R Weibel; W Stäubli; H R Gnägi; F A Hess
Journal:  J Cell Biol       Date:  1969-07       Impact factor: 10.539

10.  The nucleoids of rat liver cell microbodies. Fine structure and enzymes.

Authors:  H Tsukada; Y Mochizuki; S Fujiwara
Journal:  J Cell Biol       Date:  1966-03       Impact factor: 10.539
View more
  4 in total

1.  The catalytic activity of the endoplasmic reticulum-resident protein microsomal epoxide hydrolase towards carcinogens is retained on inversion of its membrane topology.

Authors:  T Friedberg; R Holler; B Löllmann; M Arand; F Oesch
Journal:  Biochem J       Date:  1996-10-01       Impact factor: 3.857

2.  The microsomal epoxide hydrolase has a single membrane signal anchor sequence which is dispensable for the catalytic activity of this protein.

Authors:  T Friedberg; B Löllmann; R Becker; R Holler; F Oesch
Journal:  Biochem J       Date:  1994-11-01       Impact factor: 3.857

3.  Relationship between the microsomal epoxide hydrolase and the hepatocellular transport of bile acids and xenobiotics.

Authors:  W Honscha; H D Platte; F Oesch; T Friedberg
Journal:  Biochem J       Date:  1995-11-01       Impact factor: 3.857

4.  Epoxide hydrolase is a marker for the smooth endoplasmic reticulum in rat liver.

Authors:  M M Galteau; B Antoine; H Reggio
Journal:  EMBO J       Date:  1985-11       Impact factor: 11.598
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.