Literature DB >> 80396

Concanavalin A-peroxidase-diaminobenzidine-periodic acid-m-aminophenol-fast black salt K: a method for the dual staining of neutral complex carbohydrates.

K Yamada.   

Abstract

A method has been developed for the dual staining of neutral complex carbohydrates in light microscopy. It combines a concanavalin A-peroxidase-diaminobenzidine (Con A-PO-DAB) method with a period acid-m-aminophenol-Fast Black salt K (PA-AP-FBK) sequence. With the combined method it is possible to stain alpha-D-glycosyl and alpha-D-mannosyl residues brown and 1,2-glycol groups of neutral complex carbohydrates blackish purple. The validity of the method has been confirmed with appropriate histochemical controls and enzyme digestions on test tissues.

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Year:  1978        PMID： 80396     DOI： 10.1007/BF01003138

Source DB:  PubMed          Journal:  Histochem J        ISSN： 0018-2214


  10 in total

1.  Effects of fixation on carbohydrate histochemistry.

Authors:  J F McMANUS; R W MOWRY
Journal:  J Histochem Cytochem       Date:  1958-09       Impact factor: 2.479

2.  The histochemistry of galactose residues of complex carbohydrates as studied by peroxidase-labeled Ricinus communis agglutinin.

Authors:  K Yamada; S Shimizu
Journal:  Histochemistry       Date:  1977-08-01

3.  Black periodic and black Bauer methods for tissue polysaccharides.

Authors:  R D LILLIE; P R GILMER; R A WELSH
Journal:  Stain Technol       Date:  1961-11

4.  Morphochemical analysis of mucous cells in the skin and slime glands of hagfishes.

Authors:  T J Leppi
Journal:  Histochemie       Date:  1968

5.  Letter: Destruction of endogenous peroxidase activity in order to locate cellular antigens by peroxidase-labeled antibodies.

Authors:  A Pitts; E E Williams
Journal:  J Histochem Cytochem       Date:  1974-01       Impact factor: 2.479

6.  Histochemical detection of the -D-arabinopyranoside configuration using fluorescent-labelled concanavalin A.

Authors:  R W Stoddart; J A Kiernan
Journal:  Histochemie       Date:  1973

7.  Localization of alpha-D-glucosyl and alpha-D-mannosyl groups of mucosubstances with concanavalin A and horseradish peroxidase.

Authors:  J A Kiernan
Journal:  Histochemistry       Date:  1975-07-16

8.  [Use of concanavallin-A for isolation, detection and measurement of extra- or endo-cellular glycoproteins and glucides].

Authors:  S Avrameas
Journal:  C R Acad Hebd Seances Acad Sci D       Date:  1970-05-04

9.  Cytochemical variation of mast cells in the skin of the rat.

Authors:  M Lampe; J A Kiernan
Journal:  Arch Dermatol Res       Date:  1977-03-25       Impact factor: 3.017

10.  Concanavalin A-perioxidase-diaminobenzidine (Con-A-PO-DAB)-alcian blue (AB): a reliable method for dual staining of complex carbohydrates.

Authors:  K Yamada
Journal:  Histochemistry       Date:  1976-05-28
  10 in total
  6 in total

1.  The histochemistry of complex carbohydrates in the testes of patients of idiopathic male infertility.

Authors:  Y Maseki
Journal:  Histochemistry       Date:  1979

2.  The use of peroxidase-labelled Limulus polyphemus agglutinin for the histochemistry of sialic acid-containing glycoproteins in light microscopy.

Authors:  K Yamada; S Shimizu
Journal:  Histochem J       Date:  1979-07

3.  A sensitive method for the histochemical demonstration of vicinal diols of carbohydrates.

Authors:  M Nakamura; H Kitamura; K Yamada
Journal:  Histochem J       Date:  1985-04

4.  A dual staining method for neutral complex carbohydrates using alkaline phosphatase-labeled concanavalin A and periodic acid-Schiff.

Authors:  K Yamada; H Kitamura; Y Maseki
Journal:  Histochemistry       Date:  1981

Review 5.  Histochemistry of carbohydrates as performed by physical development procedures.

Authors:  K Yamada
Journal:  Histochem J       Date:  1993-02

6.  Lectin-binding in normal and fibrillated articular cartilage of human patellae.

Authors:  M Schünke; U Schumacher; B Tillmann
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1985
  6 in total

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