Literature DB >> 6167536

A dual staining method for neutral complex carbohydrates using alkaline phosphatase-labeled concanavalin A and periodic acid-Schiff.

K Yamada, H Kitamura, Y Maseki.   

Abstract

A method has been developed for the dual staining of neutral complex carbohydrates in light microscopy. It combines an alkaline phosphatase-labeled concanavalin A-5-bromo-3-indolyl phosphate, p-toluidine salt (Con A-ALP-BIPT) method with periodic acid-Schiff (PAS) sequence. With the present dual staining method, it is possible to color alpha-D-glucosyl and alpha-D-mannosyl residues blue and 1,2-glycol groups of neutral complex carbohydrates magenta. The validity of this method has been confirmed with appropriate histochemical controls and enzyme digestions on test tissues.

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Year:  1981        PMID： 6167536     DOI： 10.1007/BF00508377

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  9 in total

1.  A correlative study of the histochemical properties of rodent acid mucopolysaccharides.

Authors:  S S SPICER
Journal:  J Histochem Cytochem       Date:  1960-01       Impact factor: 2.479

2.  Effects of fixation on carbohydrate histochemistry.

Authors:  J F McMANUS; R W MOWRY
Journal:  J Histochem Cytochem       Date:  1958-09       Impact factor: 2.479

3.  The histochemistry of galactose residues of complex carbohydrates as studied by peroxidase-labeled Ricinus communis agglutinin.

Authors:  K Yamada; S Shimizu
Journal:  Histochemistry       Date:  1977-08-01

4.  Histological and histochemical uses of periodic acid.

Authors:  J F A McMANUS
Journal:  Stain Technol       Date:  1948-07

5.  Localization of concanavalin A binding sites with a concanavalin A-alkaline phosphatase method.

Authors:  S Shimizu; K Yamada
Journal:  Okajimas Folia Anat Jpn       Date:  1979-10

6.  Morphochemical analysis of mucous cells in the skin and slime glands of hagfishes.

Authors:  T J Leppi
Journal:  Histochemie       Date:  1968

7.  Histochemical detection of the -D-arabinopyranoside configuration using fluorescent-labelled concanavalin A.

Authors:  R W Stoddart; J A Kiernan
Journal:  Histochemie       Date:  1973

8.  Cytochemical variation of mast cells in the skin of the rat.

Authors:  M Lampe; J A Kiernan
Journal:  Arch Dermatol Res       Date:  1977-03-25       Impact factor: 3.017

9.  Concanavalin A-peroxidase-diaminobenzidine-periodic acid-m-aminophenol-fast black salt K: a method for the dual staining of neutral complex carbohydrates.

Authors:  K Yamada
Journal:  Histochem J       Date:  1978-09
  9 in total

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