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Literature DB >> 893144

The histochemistry of galactose residues of complex carbohydrates as studied by peroxidase-labeled Ricinus communis agglutinin.

Abstract

A peroxidase-labeled Ricinus communis agglutinin diaminobenzidine (PO-RCA-DAB) procedure has been utilized to determine the light microscopic localization of galactose residues of complex carbohydrates in a variety of tissues from different vertebrate species. In the same tissues, the localization of mannose and glucose residues was studied by means of a concanavalin A peroxidase diaminobenzidine (Con A-PO-DAB) procedure for comparison. The distribution pattern of galactose residues was found to be in distinct contrast with that of mannose and glucose residues in connective and epithelial tissues such as those involved in the umbilical cord, cartilage, aorta, comb, thyroid gland, submaxillary gland, duodenum, jejunum, colon and gill of various vertebrates. The results of the present study indicate that the PO-RCA-DAB procedure is useful in providing a means of dividing periodic acid-Schiff reactive neutral complex carbohydrates into subgroups in comparison with the intensity of their Con A-PO-DAB reaction in the histochemistry of complex carbohydrates.

Entities: Chemical Disease Gene Species

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Year: 1977 PMID： 893144 DOI： 10.1007/bf00498489

Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564

27 in total

1. Distribution and nature of saccharides of plasma membranes of rat ascites hepatoma cells as detected by ferritin-conjugated lectins and dialysed iron.

Authors: S Shimizu; K Yamada
Journal: Exp Cell Res Date: 1976-02 Impact factor: 3.905

2. Letter: Destruction of endogenous peroxidase activity in order to locate cellular antigens by peroxidase-labeled antibodies.

Authors: A Pitts; E E Williams
Journal: J Histochem Cytochem Date: 1974-01 Impact factor: 2.479

3. The effect of digestion with chondroitinases upon certain histochemical reactions of mucosaccharide-containing tissues.

Authors: K Yamada
Journal: J Histochem Cytochem Date: 1974-04 Impact factor: 2.479

4. The effect of digestion with Streptomyces hyaluronidase upon certain histochemical reactions of hyaluronic acid-containing tissues.

Authors: K Yamada
Journal: J Histochem Cytochem Date: 1973-09 Impact factor: 2.479

5. Ultrastructural visualization of cellular carbohydrate components by means of concanavalin A.

Authors: W Bernhard; S Avrameas
Journal: Exp Cell Res Date: 1971-01 Impact factor: 3.905

6. Aminotriazole goiter. Fine structure and localization of thyroid peroxidase activity.

Authors: J M Strum; M J Karnovsky
Journal: Lab Invest Date: 1971-01 Impact factor: 5.662

7. [Use of concanavallin-A for isolation, detection and measurement of extra- or endo-cellular glycoproteins and glucides].

Authors: S Avrameas
Journal: C R Acad Hebd Seances Acad Sci D Date: 1970-05-04

8. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.

Authors: R C Graham; M J Karnovsky
Journal: J Histochem Cytochem Date: 1966-04 Impact factor: 2.479

9. Ferritin-conjugated plant agglutinins as specific saccharide stains for electron microscopy: application to saccharides bound to cell membranes.

Authors: G L Nicolson; S J Singer
Journal: Proc Natl Acad Sci U S A Date: 1971-05 Impact factor: 11.205

5. The use of peroxidase-labelled Limulus polyphemus agglutinin for the histochemistry of sialic acid-containing glycoproteins in light microscopy.

Authors: K Yamada; S Shimizu
Journal: Histochem J Date: 1979-07

6. A dual staining method for neutral complex carbohydrates using alkaline phosphatase-labeled concanavalin A and periodic acid-Schiff.

Authors: K Yamada; H Kitamura; Y Maseki
Journal: Histochemistry Date: 1981

7. Histochemical demonstration of asparagine-linked oligosaccharides in glycoproteins of human placenta and umbilical cord tissues by means of almond glycopeptidase digestion.

Authors: K Yamada; S Shimizu; N Takahashi
Journal: Histochem J Date: 1983-12

The histochemistry of galactose residues of complex carbohydrates as studied by peroxidase-labeled Ricinus communis agglutinin.

1. Distribution and nature of saccharides of plasma membranes of rat ascites hepatoma cells as detected by ferritin-conjugated lectins and dialysed iron.

2. Letter: Destruction of endogenous peroxidase activity in order to locate cellular antigens by peroxidase-labeled antibodies.

3. The effect of digestion with chondroitinases upon certain histochemical reactions of mucosaccharide-containing tissues.

4. The effect of digestion with Streptomyces hyaluronidase upon certain histochemical reactions of hyaluronic acid-containing tissues.

5. Ultrastructural visualization of cellular carbohydrate components by means of concanavalin A.

6. Aminotriazole goiter. Fine structure and localization of thyroid peroxidase activity.

7. [Use of concanavallin-A for isolation, detection and measurement of extra- or endo-cellular glycoproteins and glucides].

8. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.

9. Ferritin-conjugated plant agglutinins as specific saccharide stains for electron microscopy: application to saccharides bound to cell membranes.

10. Cytochemical localization of endogenous peroxidase in thyroid follicular cells.

1. Embryonal feather growth in the chicken.

2. The histochemistry of complex carbohydrates in the testes of patients of idiopathic male infertility.

3. The cytochemistry of glycoconjugates in the zona pellucida of murine ovarian oocytes and two-cell embryos.

4. Histochemistry of complex carbohydrates in the hairy skin of the domestic pig.

5. The use of peroxidase-labelled Limulus polyphemus agglutinin for the histochemistry of sialic acid-containing glycoproteins in light microscopy.

6. A dual staining method for neutral complex carbohydrates using alkaline phosphatase-labeled concanavalin A and periodic acid-Schiff.

7. Histochemical demonstration of asparagine-linked oligosaccharides in glycoproteins of human placenta and umbilical cord tissues by means of almond glycopeptidase digestion.

8. Glycoconjugates in the secretory epithelium of the chicken mandibular gland.

9. Complex carbohydrates in the secretory epithelium of the goat prostate.

10. The histochemistry of complex carbohydrates in the scrotum of the boar.