Literature DB >> 8038389

Measurement of the individual pKa values of acidic residues of hen and turkey lysozymes by two-dimensional 1H NMR.

K Bartik1, C Redfield, C M Dobson.   

Abstract

The pH dependence of the two-dimensional 1H nuclear magnetic resonance spectra of hen and turkey egg-white lysozymes has been recorded over the pH range 1-7. By monitoring the chemical shifts of the resonances of the various protons of ionizable residues, individual pKa values for the acidic residues have been determined for both proteins. The pKa values are displaced, with the exception of those of the residues in the active site cleft, by an average of 1 unit to low pH compared to model compounds.

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Year:  1994        PMID： 8038389      PMCID： PMC1275825          DOI： 10.1016/S0006-3495(94)80900-2

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  12 in total

1.  pKa's of ionizable groups in proteins: atomic detail from a continuum electrostatic model.

Authors:  D Bashford; M Karplus
Journal:  Biochemistry       Date:  1990-11-06       Impact factor: 3.162

2.  Electrostatic effects and hydrogen exchange behaviour in proteins. The pH dependence of exchange rates in lysozyme.

Authors:  M Delepierre; C M Dobson; M Karplus; F M Poulsen; D J States; R E Wedin
Journal:  J Mol Biol       Date:  1987-09-05       Impact factor: 5.469

Review 3.  Electrostatic interactions in macromolecules: theory and applications.

Authors:  K A Sharp; B Honig
Journal:  Annu Rev Biophys Biophys Chem       Date:  1990

4.  Sequential 1H NMR assignments and secondary structure of hen egg white lysozyme in solution.

Authors:  C Redfield; C M Dobson
Journal:  Biochemistry       Date:  1988-01-12       Impact factor: 3.162

5.  Crystallographic studies of the activity of hen egg-white lysozyme.

Authors:  C C Blake; L N Johnson; G A Mair; A C North; D C Phillips; V R Sarma
Journal:  Proc R Soc Lond B Biol Sci       Date:  1967-04-18

6.  1H-NMR analysis of turkey egg-white lysozyme and comparison with hen egg-white lysozyme.

Authors:  K Bartik; C M Dobson; C Redfield
Journal:  Eur J Biochem       Date:  1993-07-15

7.  Enzymatic activity of avian egg-white lysozymes.

Authors:  T Fukamizo; T Torikata; T Nagayama; T Minematsu; K Hayashi
Journal:  J Biochem       Date:  1983-07       Impact factor: 3.387

8.  Electrostatic effects in proteins.

Authors:  M F Perutz
Journal:  Science       Date:  1978-09-29       Impact factor: 47.728

9.  Analysis of the acid-base titration curve of hen lysozyme.

Authors:  S Kuramitsu; K Hamaguchi
Journal:  J Biochem       Date:  1980-04       Impact factor: 3.387

10.  Study of the tryptophan residues of lysozyme using 1H nuclear magnetic resonance.

Authors:  R Cassels; C M Dobson; F M Poulsen; R J Williams
Journal:  Eur J Biochem       Date:  1978-12-01
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  34 in total

1.  A self-consistent, microenvironment modulated screened coulomb potential approximation to calculate pH-dependent electrostatic effects in proteins.

Authors:  E L Mehler; F Guarnieri
Journal:  Biophys J       Date:  1999-07       Impact factor: 4.033

2.  Highly perturbed pKa values in the unfolded state of hen egg white lysozyme.

Authors:  John Bradley; Fergal O'Meara; Damien Farrell; Jens Erik Nielsen
Journal:  Biophys J       Date:  2012-04-03       Impact factor: 4.033

3.  Calculating pKa values in the cAMP-dependent protein kinase: the effect of conformational change and ligand binding.

Authors:  Una Bjarnadottir; Jens Erik Nielsen
Journal:  Protein Sci       Date:  2010-12       Impact factor: 6.725

4.  Chemically accurate protein structures: validation of protein NMR structures by comparison of measured and predicted pKa values.

Authors:  N Powers; Jan H Jensen
Journal:  J Biomol NMR       Date:  2006-06-03       Impact factor: 2.835

5.  Accurate, conformation-dependent predictions of solvent effects on protein ionization constants.

Authors:  P Barth; T Alber; P B Harbury
Journal:  Proc Natl Acad Sci U S A       Date:  2007-03-14       Impact factor: 11.205

6.  A fast and accurate computational approach to protein ionization.

Authors:  Velin Z Spassov; Lisa Yan
Journal:  Protein Sci       Date:  2008-08-19       Impact factor: 6.725

7.  Large-scale modulation of thermodynamic protein folding barriers linked to electrostatics.

Authors:  Oyvind Halskau; Raul Perez-Jimenez; Beatriz Ibarra-Molero; Jarl Underhaug; Victor Muñoz; Aurora Martinez; Jose M Sanchez-Ruiz
Journal:  Proc Natl Acad Sci U S A       Date:  2008-06-11       Impact factor: 11.205

8.  Protein dielectric constants determined from NMR chemical shift perturbations.

Authors:  Predrag Kukic; Damien Farrell; Lawrence P McIntosh; Bertrand García-Moreno E; Kristine Steen Jensen; Zigmantas Toleikis; Kaare Teilum; Jens Erik Nielsen
Journal:  J Am Chem Soc       Date:  2013-10-31       Impact factor: 15.419

9.  Modeling the electrophoresis of lysozyme. II. Inclusion of ion relaxation.

Authors:  S A Allison; M Potter; J A McCammon
Journal:  Biophys J       Date:  1997-07       Impact factor: 4.033

10.  Incorporating protein conformational flexibility into the calculation of pH-dependent protein properties.

Authors:  E G Alexov; M R Gunner
Journal:  Biophys J       Date:  1997-05       Impact factor: 4.033
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