Literature DB >> 8027328

Effect of delayed processing of blood samples on performance of cytomegalovirus antigenemia assay.

J Niubò1, J L Pérez, A Carvajal, C Ardanuy, R Martín.   

Abstract

This prospective, parallel, and blind study of 120 blood samples from immunocompromised patients examined the influence of delayed sample processing on cytomegalovirus antigenemia assay. Cytomegalovirus was detected in 49 samples (40.8%): 41 were processed within 2 to 4 h, and 40 were reprocessed the following day. Results were discrepant in 17 of the samples with the lowest positive cell counts. Differences between the two days did not reach statistical significance.

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Year:  1994        PMID： 8027328      PMCID： PMC267202          DOI： 10.1128/jcm.32.4.1119-1120.1994

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  11 in total

Review 1.  Direct detection of cytomegalovirus in peripheral blood leukocytes--a review of the antigenemia assay and polymerase chain reaction.

Authors:  T H The; M van der Ploeg; A P van den Berg; A M Vlieger; M van der Giessen; W J van Son
Journal:  Transplantation       Date:  1992-08       Impact factor: 4.939

2.  Cytomegalovirus antigenemia assay.

Authors:  M van der Giessen; T H The; W J van Son
Journal:  J Clin Microbiol       Date:  1991-12       Impact factor: 5.948

3.  Prospective study of cytomegalovirus antigenemia in allograft recipients.

Authors:  H Miller; E Rossier; R Milk; C Thomas
Journal:  J Clin Microbiol       Date:  1991-05       Impact factor: 5.948

Review 4.  Cytomegalovirus antigenemia.

Authors:  T H The; W van der Bij; A P van den Berg; M van der Giessen; J Weits; H G Sprenger; W J van Son
Journal:  Rev Infect Dis       Date:  1990 Sep-Oct

5.  Specimens for cytomegalovirus antigenemia assay.

Authors:  F J Michalski
Journal:  J Clin Microbiol       Date:  1993-05       Impact factor: 5.948

6.  Detection of human cytomegalovirus immediate early antigen in leukocytes as a marker of viremia in immunocompromised patients.

Authors:  M G Revello; E Percivalle; M Zavattoni; M Parea; P Grossi; G Gerna
Journal:  J Med Virol       Date:  1989-10       Impact factor: 2.327

7.  The detection of human cytomegalovirus immediate early antigen in peripheral blood leucocytes.

Authors:  G Bein; A Bitsch; J Hoyer; H Kirchner
Journal:  J Immunol Methods       Date:  1991-03-21       Impact factor: 2.303

8.  Cytomegalovirus (CMV) antigenemia assay is more sensitive than shell vial cultures for rapid detection of CMV in polymorphonuclear blood leukocytes.

Authors:  A Erice; M A Holm; P C Gill; S Henry; C L Dirksen; D L Dunn; R P Hillam; H H Balfour
Journal:  J Clin Microbiol       Date:  1992-11       Impact factor: 5.948

9.  Comparison between viremia and antigenemia for detection of cytomegalovirus in blood.

Authors:  W van der Bij; J Schirm; R Torensma; W J van Son; A M Tegzess; T H The
Journal:  J Clin Microbiol       Date:  1988-12       Impact factor: 5.948

10.  Comparison of different immunostaining techniques and monoclonal antibodies to the lower matrix phosphoprotein (pp65) for optimal quantitation of human cytomegalovirus antigenemia.

Authors:  G Gerna; M G Revello; E Percivalle; F Morini
Journal:  J Clin Microbiol       Date:  1992-05       Impact factor: 5.948
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  8 in total

Review 1.  Real-time PCR in virology.

Authors:  Ian M Mackay; Katherine E Arden; Andreas Nitsche
Journal:  Nucleic Acids Res       Date:  2002-03-15       Impact factor: 16.971

2.  Effect of delayed specimen processing on cytomegalovirus antigenemia test results.

Authors:  M L Landry; D Ferguson; S Cohen; K Huber; P Wetherill
Journal:  J Clin Microbiol       Date:  1995-01       Impact factor: 5.948

3.  Comparison of culture and the antigenemia assay for detection of cytomegalovirus in blood specimens submitted to a reference laboratory.

Authors:  B G Brumback; S N Bolejack; M V Morris; C Mohla; T E Shutzbank
Journal:  J Clin Microbiol       Date:  1997-07       Impact factor: 5.948

4.  Minimal effect of delayed sample processing on results of quantitative PCR for cytomegalovirus DNA in leukocytes compared to results of an antigenemia assay.

Authors:  P Schäfer; W Tenschert; K Gutensohn; R Laufs
Journal:  J Clin Microbiol       Date:  1997-03       Impact factor: 5.948

5.  Effects of storage temperature and time on qualitative and quantitative detection of cytomegalovirus in blood specimens by shell vial culture and PCR.

Authors:  T C Roberts; R S Buller; M Gaudreault-Keener; K E Sternhell; K Garlock; G G Singer; D C Brennan; G A Storch
Journal:  J Clin Microbiol       Date:  1997-09       Impact factor: 5.948

6.  Comparison of EDTA and acid-citrate-dextrose collection tubes for detection of cytomegalovirus antigenemia and infectivity in leukocytes before and after storage.

Authors:  M L Landry; S Cohen; K Huber
Journal:  J Clin Microbiol       Date:  1997-01       Impact factor: 5.948

7.  Comparison of several fixation methods for cytomegalovirus antigenemia assay.

Authors:  J L Pérez; M De Oña; J Niubò; H Villar; S Melón; A García; R Martín
Journal:  J Clin Microbiol       Date:  1995-06       Impact factor: 5.948

8.  Standardization of the human cytomegalovirus antigenemia assay by means of in vitro-generated pp65-positive peripheral blood polymorphonuclear leukocytes.

Authors:  G Gerna; E Percivalle; M Torsellini; M G Revello
Journal:  J Clin Microbiol       Date:  1998-12       Impact factor: 5.948
  8 in total

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