Literature DB >> 9041425

Minimal effect of delayed sample processing on results of quantitative PCR for cytomegalovirus DNA in leukocytes compared to results of an antigenemia assay.

P Schäfer1, W Tenschert, K Gutensohn, R Laufs.   

Abstract

Quantitative cytomegalovirus antigenemia and DNAemia were determined in peripheral leukocytes of 25 patients stored for up to 72 h at room temperature (RT) and 4 degrees C before processing. Numbers of antigen-positive cells significantly decreased with time. The decline was greater at RT than at 4 degrees C. In contrast, no significant alterations in DNAemia occurred.

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Year:  1997        PMID： 9041425      PMCID： PMC229663          DOI： 10.1128/jcm.35.3.741-744.1997

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  23 in total

1.  Cytomegalovirus antigenemia as a useful marker of symptomatic cytomegalovirus infection after renal transplantation--a report of 130 consecutive patients.

Authors:  A P van den Berg; W van der Bij; W J van Son; J Anema; M van der Giessen; J Schirm; A M Tegzess; T H The
Journal:  Transplantation       Date:  1989-12       Impact factor: 4.939

2.  Prospective study of cytomegalovirus antigenemia in allograft recipients.

Authors:  H Miller; E Rossier; R Milk; C Thomas
Journal:  J Clin Microbiol       Date:  1991-05       Impact factor: 5.948

3.  Antigenemia in the diagnosis and monitoring of active cytomegalovirus infection after liver transplantation.

Authors:  A P van den Berg; I J Klompmaker; E B Haagsma; A Scholten-Sampson; C M Bijleveld; J Schirm; M van der Giessen; M J Slooff; T H The
Journal:  J Infect Dis       Date:  1991-08       Impact factor: 5.226

Review 4.  Cytomegalovirus antigenemia.

Authors:  T H The; W van der Bij; A P van den Berg; M van der Giessen; J Weits; H G Sprenger; W J van Son
Journal:  Rev Infect Dis       Date:  1990 Sep-Oct

Review 5.  The cytomegalovirus antigenemia assay: a plea for standardization.

Authors:  T H The; A P van den Berg; M C Harmsen; W van der Bij; W J van Son
Journal:  Scand J Infect Dis Suppl       Date:  1995

6.  Factors influencing detection of quantitative cytomegalovirus antigenemia.

Authors:  M Boeckh; P M Woogerd; T Stevens-Ayers; C G Ray; R A Bowden
Journal:  J Clin Microbiol       Date:  1994-03       Impact factor: 5.948

7.  Prospective study utilizing a quantitative polymerase chain reaction for detection of cytomegalovirus DNA in the blood of renal transplant patients.

Authors:  J C Gerdes; E K Spees; K Fitting; J Hiraki; M Sheehan; D Duda; T Jarvi; C Roehl; A D Robertson
Journal:  Transplant Proc       Date:  1993-02       Impact factor: 1.066

8.  Quantitative determination of human cytomegalovirus target sequences in peripheral blood leukocytes by nested polymerase chain reaction and temperature gradient gel electrophoresis.

Authors:  P Schäfer; R W Braun; K Möhring; K Henco; J Kang; T Wendland; J E Kühn
Journal:  J Gen Virol       Date:  1993-12       Impact factor: 3.891

9.  Quantification of human cytomegalovirus DNA in peripheral blood polymorphonuclear leukocytes of immunocompromised patients by the polymerase chain reaction.

Authors:  D Zipeto; F Baldanti; D Zella; M Furione; A Cavicchini; G Milanesi; G Gerna
Journal:  J Virol Methods       Date:  1993-09       Impact factor: 2.014

10.  Comparison between viremia and antigenemia for detection of cytomegalovirus in blood.

Authors:  W van der Bij; J Schirm; R Torensma; W J van Son; A M Tegzess; T H The
Journal:  J Clin Microbiol       Date:  1988-12       Impact factor: 5.948
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  20 in total

1.  Comparison of PCR, antigenemia assay, and rapid blood culture for detection and prevention of cytomegalovirus disease after lung transplantation.

Authors:  A Weinberg; T N Hodges; S Li; G Cai; M R Zamora
Journal:  J Clin Microbiol       Date:  2000-02       Impact factor: 5.948

2.  Enhanced analytical sensitivity of a quantitative PCR for CMV using a modified nucleic-acid extraction procedure.

Authors:  A Ferreira-Gonzalez; S Yanovich; M R Langley; L A Weymouth; D S Wilkinson; C T Garrett
Journal:  J Clin Lab Anal       Date:  2000       Impact factor: 2.352

3.  2-Hour cytomegalovirus pp65 antigenemia assay for rapid quantitation of cytomegalovirus in blood samples.

Authors:  M L Landry; D Ferguson
Journal:  J Clin Microbiol       Date:  2000-01       Impact factor: 5.948

4.  Optimization of quantitative detection of cytomegalovirus DNA in plasma by real-time PCR.

Authors:  Michael Boeckh; MeeiLi Huang; James Ferrenberg; Terry Stevens-Ayers; Laurence Stensland; W Garrett Nichols; Lawrence Corey
Journal:  J Clin Microbiol       Date:  2004-03       Impact factor: 5.948

5.  Human cytomegalovirus DNA in plasma and serum specimens of renal transplant recipients is highly fragmented.

Authors:  René Boom; Cees J A Sol; Tim Schuurman; Alex Van Breda; Jan F L Weel; Marcel Beld; Ineke J M Ten Berge; Pauline M E Wertheim-Van Dillen; Menno D De Jong
Journal:  J Clin Microbiol       Date:  2002-11       Impact factor: 5.948

Review 6.  New strategies for prevention and therapy of cytomegalovirus infection and disease in solid-organ transplant recipients.

Authors:  I G Sia; R Patel
Journal:  Clin Microbiol Rev       Date:  2000-01       Impact factor: 26.132

7.  Overview of the diagnosis of cytomegalovirus infection.

Authors:  S A Ross; Z Novak; S Pati; S B Boppana
Journal:  Infect Disord Drug Targets       Date:  2011-10

8.  False-positive results of plasma PCR for cytomegalovirus DNA due to delayed sample preparation.

Authors:  P Schäfer; W Tenschert; M Schröter; K Gutensohn; R Laufs
Journal:  J Clin Microbiol       Date:  2000-09       Impact factor: 5.948

Review 9.  Current management strategies for the prevention and treatment of cytomegalovirus infection in pediatric transplant recipients.

Authors:  Javier Bueno; Carmen Ramil; Michael Green
Journal:  Paediatr Drugs       Date:  2002       Impact factor: 3.022

Review 10.  Clinical utility of viral load in management of cytomegalovirus infection after solid organ transplantation.

Authors:  Raymund R Razonable; Randall T Hayden
Journal:  Clin Microbiol Rev       Date:  2013-10       Impact factor: 26.132
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