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Literature DB >> 800403

Isolation of human eosinophils and their lymphocyte-like rosetting properties.

Abstract

A method for the isolation of large numbers of almost pure human eosinophils from peripheral blood using hypague-ficoll density gradients, gelatin sedimentation and carbonyl iron is described. Eosinophils were obtained from patients with parasitic infections, allergic reactions, undiagnosed eosinophilias and normal volunteers. Erythrocyte (E) and erythrocyte-antibody-complement (EAC) rosettes were formed by a number of eosinophils.

Entities: Chemical Disease Species

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Year: 1976 PMID： 800403 PMCID： PMC1445251

Source DB: PubMed Journal: Immunology ISSN： 0019-2805 Impact factor: 7.397

6 in total

1. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g.

Authors: A Böyum
Journal: Scand J Clin Lab Invest Suppl Date: 1968

2. An extension of the 51Cr-release assay for the estimation of mouse cytotoxins.

Authors: W Boyle
Journal: Transplantation Date: 1968-09 Impact factor: 4.939

3. Eosinophil cell separation from human peripheral blood.

Authors: R P Day
Journal: Immunology Date: 1970-06 Impact factor: 7.397

4. T and CR+ lymphocyte profile in leprosy and the effect of treatment.

Authors: R Sher; G Holm; S H Kok; H J Koornhof; A Glover
Journal: Infect Immun Date: 1976-01 Impact factor: 3.441

5. Role of the eosinophil in the allergic reactions. I. EDI-an eosinophil-derived inhibitor of histamine release.

Authors: T Hubscher
Journal: J Immunol Date: 1975-04 Impact factor: 5.422

6. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells.

Authors: M Jondal; G Holm; H Wigzell
Journal: J Exp Med Date: 1972-08-01 Impact factor: 14.307

6 in total

10 in total

1. Purification of normal human eosinophils using the different binding capacities of blood leucocytes for complexed rabbit IgG.

Authors: P C Tai; C J Spry
Journal: Clin Exp Immunol Date: 1977-05 Impact factor: 4.330

Review 2. Eosinophils. Assays and interpretation.

Authors: D E Maddox
Journal: Clin Rev Allergy Date: 1988

3. Eosinophil degranulation. Monitoring by interference contrast microscopy.

Authors: R Sher; A A Wadee
Journal: Inflammation Date: 1981-03 Impact factor: 4.092

4. An improved method for the isolation of eosinophilic leukocytes from normal human blood.

Authors: P Portmann; P Köppel; A Jörg
Journal: Experientia Date: 1980-01-15

5. Antibody-dependent cellular cytotoxicity of Trypanosoma cruzi: characterization of the effector cell from normal human blood.

Authors: E D Madeira; A F de Andrade; M M Bunn-Moreno; M Barcinski
Journal: Infect Immun Date: 1979-07 Impact factor: 3.441

6. Human eosinophils and parasitic diseases: light and electron microscopy evidence of interaction with sheep erythrocyte.

Authors: C de Simone; G Donelli; D Meli; F Rosati; F Sorice
Journal: Clin Exp Immunol Date: 1980-01 Impact factor: 4.330

7. The effects of a soluble factor released by sensitized mononuclear cells incubated with S. haematobium ova on eosinophil migration.

Authors: A A Wadee; R Sher
Journal: Immunology Date: 1980-12 Impact factor: 7.397