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Literature DB >> 7358123

An improved method for the isolation of eosinophilic leukocytes from normal human blood.

Abstract

A relatively short and simple method for the isolation of the eosinophils from normal human blood is reported. With a recovery of about 45-55%, cell preparations, showing a degree of purity of 90-98%, are obtained. The isolated cells are morphologically intact and viable, as assessed by the trypan blue exclusion test and by active phagocytosis.

Entities: Chemical Species

Mesh：

Year: 1980 PMID： 7358123 DOI： 10.1007/bf02004024

Source DB: PubMed Journal: Experientia ISSN： 0014-4754

11 in total

1. Purification of normal human eosinophils using the different binding capacities of blood leucocytes for complexed rabbit IgG.

Authors: P C Tai; C J Spry
Journal: Clin Exp Immunol Date: 1977-05 Impact factor: 4.330

2. Studies on blood eosinophils. II. Patients with Löffler's cardiomyopathy.

Authors: C J Spry; P C Tai
Journal: Clin Exp Immunol Date: 1976-06 Impact factor: 4.330

Review 3. Eosinophils as effector cells in disease.

Authors: C J Spry
Journal: Schweiz Med Wochenschr Date: 1978-10-14

4. Biological defense mechanisms. The production by leukocytes of superoxide, a potential bactericidal agent.

Authors: B M Babior; R S Kipnes; J T Curnutte
Journal: J Clin Invest Date: 1973-03 Impact factor: 14.808

5. Quantitative intranasal pollen challenge. II. Effect of daily pollen challenge, environmental pollen exposure, and placebo challenge on the nasal membrane.

Authors: J T Connell
Journal: J Allergy Date: 1968-03

6. Phagocytic properties of isolated human eosinophils.

Authors: W H Grover; H H Winkler; D E Normansell
Journal: J Immunol Date: 1978-08 Impact factor: 5.422

7. A rapid and simple method for the isolation of pure eosinophilic leukocytes from horse blood.

Authors: A Jörg; P Portmann; G Fellay; J L Dreyer; J Meyer
Journal: Experientia Date: 1978-12-15

8. Human eosinophils. Purification and cytotoxic capability of eosinophils from patients with the hypereosinophilic syndrome.

Authors: J E Parrillo; A S Fauci
Journal: Blood Date: 1978-03 Impact factor: 22.113

An improved method for the isolation of eosinophilic leukocytes from normal human blood.

1. Purification of normal human eosinophils using the different binding capacities of blood leucocytes for complexed rabbit IgG.

2. Studies on blood eosinophils. II. Patients with Löffler's cardiomyopathy.

Review 3. Eosinophils as effector cells in disease.

4. Biological defense mechanisms. The production by leukocytes of superoxide, a potential bactericidal agent.

5. Quantitative intranasal pollen challenge. II. Effect of daily pollen challenge, environmental pollen exposure, and placebo challenge on the nasal membrane.

6. Phagocytic properties of isolated human eosinophils.

7. A rapid and simple method for the isolation of pure eosinophilic leukocytes from horse blood.

8. Human eosinophils. Purification and cytotoxic capability of eosinophils from patients with the hypereosinophilic syndrome.

9. Isolation of human eosinophils and their lymphocyte-like rosetting properties.

10. Chemiluminescence and superoxide production by myeloperoxidase-deficient leukocytes.

1. Cells and inflammation: modern trends and technical outlook.

2. Isolation of bovine eosinophils and characterization of their leukotriene formation.