Literature DB >> 8001174

Highly-efficient transformation of the homobasidiomycete Schizophyllum commune to phleomycin resistance.

F H Schuren1, J G Wessels.   

Abstract

Regulatory sequences of the glyceraldehyde-3-phosphate-dehydrogenase (GPD) gene from the homobasidiomycete Schizophyllum commune were fused to the coding sequence of the ble gene from Streptoalloteichus hindustanus, which codes for a phleomycin-binding protein. The resulting construct transformed S. commune to phleomycin resistance at a high frequency (up to 10(4) transformants/microgram DNA per 10(7) protoplasts) when regeneration was done in 0.5 M MgSO4. A similar construct with regulatory sequences from Aspergillus nidulans failed to give transformants, showing the importance of homologous regulatory sequences for the expression of genes in S. commune. The homologous GPD promoter could be deleted up to position -130 without any effect on the number of phleomycin-resistant transformants. This is the first effective stable transformation system in a homobasidiomycete employing antibiotic resistance.

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Year:  1994        PMID： 8001174     DOI： 10.1007/BF00313808

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  12 in total

1.  Two genes specifically expressed in fruiting dikaryons of Schizophyllum commune: homologies with a gene not regulated by mating-type genes.

Authors:  F H Schuren; J G Wessels
Journal:  Gene       Date:  1990-06-15       Impact factor: 3.688

2.  The Sc7/Sc14 gene family of Schizophyllum commune codes for extracellular proteins specifically expressed during fruit-body formation.

Authors:  F H Schuren; S A Asgeirsdóttir; E M Kothe; J M Scheer; J G Wessels
Journal:  J Gen Microbiol       Date:  1993-09

3.  Recovery of recombinant plasmids from Pleurotus ostreatus transformants.

Authors:  M Peng; N K Singh; P A Lemke
Journal:  Curr Genet       Date:  1992-07       Impact factor: 3.886

4.  Introduction of hygromycin B resistance into Schizophyllum commune: preferential methylation of donor DNA.

Authors:  H Mooibroek; A G Kuipers; J H Sietsma; P J Punt; J G Wessels
Journal:  Mol Gen Genet       Date:  1990-06

5.  Characterization of the genome of the basidiomycete Schizophyllum commune.

Authors:  J J Dons; O M de Vries; J G Wessels
Journal:  Biochim Biophys Acta       Date:  1979-06-20

6.  Sequence analysis of the glyceraldehyde-3-phosphate dehydrogenase genes from the basidiomycetes Schizophyllum commune, Phanerochaete chrysosporium and Agaricus bisporus.

Authors:  M C Harmsen; F H Schuren; S M Moukha; C M van Zuilen; P J Punt; J G Wessels
Journal:  Curr Genet       Date:  1992-12       Impact factor: 3.886

7.  A homologous gene-reporter system for the basidiomycete Schizophyllum commune based on internally deleted homologous genes.

Authors:  F H Schuren; M C Harmsen; J G Wessels
Journal:  Mol Gen Genet       Date:  1993-04

8.  Sequence analysis of the URA1 gene encoding orotidine-5'-monophosphate decarboxylase of Schizophyllum commune.

Authors:  E H Froeliger; R C Ullrich; C P Novotny
Journal:  Gene       Date:  1989-11-30       Impact factor: 3.688

9.  Genetic transformation of the symbiotic basidiomycete fungus Hebeloma cylindrosporum.

Authors:  R Marmeisse; G Gay; J C Debaud; L A Casselton
Journal:  Curr Genet       Date:  1992-07       Impact factor: 3.886

Review 10.  Molecular biology of the lignin-degrading basidiomycete Phanerochaete chrysosporium.

Authors:  M H Gold; M Alic
Journal:  Microbiol Rev       Date:  1993-09
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  17 in total

1.  Surface modifications created by using engineered hydrophobins.

Authors:  Karin Scholtmeijer; Meike I Janssen; Bertus Gerssen; Marcel L de Vocht; Babs M van Leeuwen; Theo G van Kooten; Han A B Wösten; Joseph G H Wessels
Journal:  Appl Environ Microbiol       Date:  2002-03       Impact factor: 4.792

2.  Homology-dependent silencing of the SC3 gene in Schizophyllum commune.

Authors:  T A Schuurs; E A Schaeffer; J G Wessels
Journal:  Genetics       Date:  1997-10       Impact factor: 4.562

3.  Transient expression and insertional mutagenesis of Puccinia triticina using biolistics.

Authors:  Craig A Webb; Les J Szabo; Guus Bakkeren; Clarke Garry; Richard C Staples; Merle Eversmeyer; John P Fellers
Journal:  Funct Integr Genomics       Date:  2005-11-12       Impact factor: 3.410

4.  Effect of introns and AT-rich sequences on expression of the bacterial hygromycin B resistance gene in the basidiomycete Schizophyllum commune.

Authors:  K Scholtmeijer; H A Wösten; J Springer; J G Wessels
Journal:  Appl Environ Microbiol       Date:  2001-01       Impact factor: 4.792

5.  Development of a transformation system for Crinipellis perniciosa, the causal agent of witches' broom in cocoa plants.

Authors:  Juliana Oliveira Lima; Jildete Karla dos Santos; Jorge Fernando Pereira; Mário Lúcio Vilela de Resende; Elza Fernandes de Araújo; Marisa Vieira de Queiroz
Journal:  Curr Genet       Date:  2002-12-17       Impact factor: 3.886

6.  An efficient gene deletion procedure for the mushroom-forming basidiomycete Schizophyllum commune.

Authors:  Robin A Ohm; Jan F de Jong; Elsa Berends; Fengfeng Wang; Han A B Wösten; Luis G Lugones
Journal:  World J Microbiol Biotechnol       Date:  2010-02-27       Impact factor: 3.312

7.  Establishing molecular tools for genetic manipulation of the pleuromutilin-producing fungus Clitopilus passeckerianus.

Authors:  Sreedhar Kilaru; Catherine M Collins; Amanda J Hartley; Andy M Bailey; Gary D Foster
Journal:  Appl Environ Microbiol       Date:  2009-09-18       Impact factor: 4.792

8.  A central role for Ras1 in morphogenesis of the basidiomycete Schizophyllum commune.

Authors:  Nicole Knabe; Elke-Martina Jung; Daniela Freihorst; Florian Hennicke; J Stephen Horton; Erika Kothe
Journal:  Eukaryot Cell       Date:  2013-04-19

9.  Investigating dominant selection markers for Coprinopsis cinerea: a carboxin resistance system and re-evaluation of hygromycin and phleomycin resistance vectors.

Authors:  Sreedhar Kilaru; Catherine M Collins; Amanda J Hartley; Claire Burns; Gary D Foster; Andy M Bailey
Journal:  Curr Genet       Date:  2009-07-28       Impact factor: 3.886

10.  RNA-mediated gene silencing in monokaryons and dikaryons of Schizophyllum commune.

Authors:  Jan F de Jong; Heine J Deelstra; Han A B Wösten; Luis G Lugones
Journal:  Appl Environ Microbiol       Date:  2006-02       Impact factor: 4.792
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