| Literature DB >> 12589475 |
Juliana Oliveira Lima1, Jildete Karla dos Santos, Jorge Fernando Pereira, Mário Lúcio Vilela de Resende, Elza Fernandes de Araújo, Marisa Vieira de Queiroz.
Abstract
Protoplasts of the pathogenic plant fungus, Crinipellis perniciosa, were transformed to hygromycin B resistance using the pAN7-1 plasmid, which contains the Escherichia coli hph gene under the control of Aspergillus nidulans regulatory sequences. The pAN7-1 plasmid was introduced by PEG/CaCl(2) treatment. Transformation frequencies of 1.6-2.5 transformants/microg of DNA were achieved. About 54% of the transformants were abortive and 40 analyzed transformants were mitotically stable and showed different hygromycin B resistance levels. The presence of the hph gene was checked by PCR in five transformants and the integration of multiple plasmid copies into different genome sites was observed by Southern analysis. This is the first report of a C. perniciosa transformation system and represents an important step for further research into genetic manipulation of this fungal plant pathogen.Entities:
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Year: 2002 PMID： 12589475 DOI： 10.1007/s00294-002-0358-z
Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886