Literature DB >> 7989558

Identification and elimination of DNA sequences in Taq DNA polymerase.

M S Hughes1, L A Beck, R A Skuce.   

Abstract

This study confirms that different preparations of Taq DNA polymerase are contaminated with eubacterial DNA. The contaminants appeared to represent more than one strain or species but were not identified as Thermus aquaticus or Escherichia coli. Differences in microcentrifuge tube composition appeared to affect elimination of the contaminants.

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Year:  1994        PMID： 7989558      PMCID： PMC263920          DOI： 10.1128/jcm.32.8.2007-2008.1994

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  5 in total

1.  Taq polymerase contains bacterial DNA of unknown origin.

Authors:  K H Rand; H Houck
Journal:  Mol Cell Probes       Date:  1990-12       Impact factor: 2.365

2.  Detection of DNA contamination in Taq polymerase.

Authors:  T M Schmidt; B Pace; N R Pace
Journal:  Biotechniques       Date:  1991-08       Impact factor: 1.993

3.  Frequent contamination of Taq polymerase with DNA.

Authors:  E C Böttger
Journal:  Clin Chem       Date:  1990-06       Impact factor: 8.327

4.  Elimination of contaminating DNA within polymerase chain reaction reagents: implications for a general approach to detection of uncultured pathogens.

Authors:  A Meier; D H Persing; M Finken; E C Böttger
Journal:  J Clin Microbiol       Date:  1993-03       Impact factor: 5.948

5.  Identification of mycobacteria from animals by restriction enzyme analysis and direct DNA cycle sequencing of polymerase chain reaction-amplified 16S rRNA gene sequences.

Authors:  M S Hughes; R A Skuce; L A Beck; S D Neill
Journal:  J Clin Microbiol       Date:  1993-12       Impact factor: 5.948
  5 in total
  38 in total

1.  Does blood of healthy subjects contain bacterial ribosomal DNA?

Authors:  S Nikkari; I J McLaughlin; W Bi; D E Dodge; D A Relman
Journal:  J Clin Microbiol       Date:  2001-05       Impact factor: 5.948

2.  Contamination and sensitivity issues with a real-time universal 16S rRNA PCR.

Authors:  C E Corless; M Guiver; R Borrow; V Edwards-Jones; E B Kaczmarski; A J Fox
Journal:  J Clin Microbiol       Date:  2000-05       Impact factor: 5.948

3.  16S ribosomal DNA-based analysis of bacterial diversity in purified water used in pharmaceutical manufacturing processes by PCR and denaturing gradient gel electrophoresis.

Authors:  Mako Kawai; Eiichi Matsutera; Hisashi Kanda; Nobuyasu Yamaguchi; Katsuji Tani; Masao Nasu
Journal:  Appl Environ Microbiol       Date:  2002-02       Impact factor: 4.792

4.  DNase pretreatment of master mix reagents improves the validity of universal 16S rRNA gene PCR results.

Authors:  Alexandra Heininger; Marlies Binder; Andreas Ellinger; Konrad Botzenhart; Klaus Unertl; Gerd Döring
Journal:  J Clin Microbiol       Date:  2003-04       Impact factor: 5.948

Review 5.  Next-generation sequencing in the analysis of human microbiota: essential considerations for clinical application.

Authors:  Geraint B Rogers; Kenneth D Bruce
Journal:  Mol Diagn Ther       Date:  2010-12-01       Impact factor: 4.074

6.  Prospective study of use of PCR amplification and sequencing of 16S ribosomal DNA from cerebrospinal fluid for diagnosis of bacterial meningitis in a clinical setting.

Authors:  Tim Schuurman; Richard F de Boer; Anna M D Kooistra-Smid; Anton A van Zwet
Journal:  J Clin Microbiol       Date:  2004-02       Impact factor: 5.948

7.  Comparison of different decontamination methods for reagents to detect low concentrations of bacterial 16S DNA by real-time-PCR.

Authors:  Sven Klaschik; Lutz E Lehmann; Ansgar Raadts; Andreas Hoeft; Frank Stuber
Journal:  Mol Biotechnol       Date:  2002-11       Impact factor: 2.695

8.  Presence of bacterial phage-like DNA sequences in commercial Taq DNA polymerase reagents.

Authors:  Tamara Newsome; Bing-Jie Li; Nianxiang Zou; Shyh-Ching Lo
Journal:  J Clin Microbiol       Date:  2004-05       Impact factor: 5.948

Review 9.  False-positive results and contamination in nucleic acid amplification assays: suggestions for a prevent and destroy strategy.

Authors:  A Borst; A T A Box; A C Fluit
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2004-03-10       Impact factor: 3.267

10.  Rapid Enrichment of Dehalococcoides-Like Bacteria by Partial Hydrophobic Separation.

Authors:  Hanna R Temme; Kipp Sande; Tao Yan; Paige J Novak
Journal:  Appl Environ Microbiol       Date:  2017-03-02       Impact factor: 4.792
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