| Literature DB >> 7957045 |
D J Hilton1, A A Hilton, A Raicevic, S Rakar, M Harrison-Smith, N M Gough, C G Begley, D Metcalf, N A Nicola, T A Willson.
Abstract
An adult mouse liver cDNA library was screened with oligonucleotides corresponding to the conserved WSXWS motif of the haemopoietin receptor family. Using this method, cDNA clones encoding a novel receptor were isolated. The new receptor, named NR1, was most similar in sequence and predicted structure to the alpha-chain of the IL-6 receptor and mRNA was expressed in the 3T3-L1 pre-adipocytic cell line and in a range of primary tissues. Expression of NR1 in the factor-dependent haemopoietic cell line Ba/F3 resulted in the generation of low affinity receptors for IL-11 (Kd approximately 10 nM). The capacity to bind IL-11 with high affinity (Kd = 300-800 pM) appeared to require coexpression of both NR1 and gp130, the common subunit of the IL-6, leukaemia inhibitory factor (LIF), oncostatin M (OSM) and ciliary neurotrophic factor (CNTF) receptors. The expression of both NR1 and gp130 was also necessary for Ba/F3 cells to proliferate and M1 cells to undergo macrophage differentiation in response to IL-11.Entities:
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Year: 1994 PMID： 7957045 PMCID： PMC395415 DOI： 10.1002/j.1460-2075.1994.tb06802.x
Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598