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Literature DB >> 7948313

Detection of IL-6 and its receptor mRNAs in a megakaryocytic cell line, CMK, by an RT-nested PCR.

Abstract

An autocrine loop in a megakaryocytic cell line, CMK, has been proposed by Fuse et al. They detected IL-6 mRNA by means of Northern blotting, but not IL-6 receptor (IL-6R) mRNA. I also failed to detect IL-6R mRNA by the same means. In this study I showed the expression of IL-6 and its receptor mRNAs by means of reverse transcriptase-nested polymerase chain reaction (RT-nested PCR) with an automated electrophoresis PhastSystem. These two procedures considerably enhanced detection sensitivity. The proliferation and the differentiation of CMK cells are augmented by IL-6 and inhibited by anti-IL-6 antibody. This study continued the autocrine theory of Fuse et al. in CMK cells. Moreover, this is the first detection of IL-6R mRNA in CMK.

Entities: Gene

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Year: 1994 PMID： 7948313 DOI： 10.1007/bf01700278

Source DB: PubMed Journal: Ann Hematol ISSN： 0939-5555 Impact factor: 3.673

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References

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Detection of IL-6 and its receptor mRNAs in a megakaryocytic cell line, CMK, by an RT-nested PCR.

1. Stimulation of thrombopoiesis in mice by human recombinant interleukin 6.

2. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

3. Direct sequencing of enzymatically amplified human genomic DNA.

4. Simple, sensitive, and specific detection of human immunodeficiency virus type 1 in clinical specimens by polymerase chain reaction with nested primers.

5. Cloning and expression of the human interleukin-6 (BSF-2/IFN beta 2) receptor.

6. Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin.

7. Quantitation of mRNA by the polymerase chain reaction.

8. Interleukin 6, a possible autocrine growth and differentiation factor for the human megakaryocytic cell line, CMK.

9. Regulation of megakaryocyte development by interleukin-6.

10. Receptors for B cell stimulatory factor 2. Quantitation, specificity, distribution, and regulation of their expression.