Literature DB >> 7929016

Gene fusion analysis of membrane protein topology: a direct comparison of alkaline phosphatase and beta-lactamase fusions.

W A Prinz1, J Beckwith.   

Abstract

To compare two approaches to analyzing membrane protein topology, a number of alkaline phosphatase fusions to membrane proteins were converted to beta-lactamase fusions. While some alkaline phosphatase fusions near the N terminus of cytoplasmic loops of membrane proteins have anomalously high levels of activity, the equivalent beta-lactamase fusions do not. This disparity may reflect differences in the folding of beta-lactamase and alkaline phosphatase in the cytoplasm.

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Year:  1994        PMID： 7929016      PMCID： PMC196986          DOI： 10.1128/jb.176.20.6410-6413.1994

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  27 in total

1.  Retardation of folding as a possible means of suppression of a mutation in the leader sequence of an exported protein.

Authors:  G P Liu; T B Topping; W H Cover; L L Randall
Journal:  J Biol Chem       Date:  1988-10-15       Impact factor: 5.157

2.  A genetic approach to analyzing membrane protein topology.

Authors:  C Manoil; J Beckwith
Journal:  Science       Date:  1986-09-26       Impact factor: 47.728

3.  Correlation of competence for export with lack of tertiary structure of the mature species: a study in vivo of maltose-binding protein in E. coli.

Authors:  L L Randall; S J Hardy
Journal:  Cell       Date:  1986-09-12       Impact factor: 41.582

4.  Suppression of a signal sequence mutation by an amino acid substitution in the mature portion of the maltose-binding protein.

Authors:  W H Cover; J P Ryan; P J Bassford; K A Walsh; J Bollinger; L L Randall
Journal:  J Bacteriol       Date:  1987-05       Impact factor: 3.490

5.  Determinants of membrane protein topology.

Authors:  D Boyd; C Manoil; J Beckwith
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

6.  A vector for the construction of translational fusions to TEM beta-lactamase and the analysis of protein export signals and membrane protein topology.

Authors:  J K Broome-Smith; B G Spratt
Journal:  Gene       Date:  1986       Impact factor: 3.688

7.  Mutations that allow disulfide bond formation in the cytoplasm of Escherichia coli.

Authors:  A I Derman; W A Prinz; D Belin; J Beckwith
Journal:  Science       Date:  1993-12-10       Impact factor: 47.728

8.  Stability of wild-type and mutant RTEM-1 beta-lactamases: effect of the disulfide bond.

Authors:  S C Schultz; G Dalbadie-McFarland; J J Neitzel; J H Richards
Journal:  Proteins       Date:  1987

9.  Mutations that alter the signal sequence of alkaline phosphatase in Escherichia coli.

Authors:  S Michaelis; H Inouye; D Oliver; J Beckwith
Journal:  J Bacteriol       Date:  1983-04       Impact factor: 3.490

10.  The precursor of beta-lactamase: purification, properties and folding kinetics.

Authors:  A A Laminet; A Plückthun
Journal:  EMBO J       Date:  1989-05       Impact factor: 11.598
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  11 in total

1.  Analysis of F factor TraD membrane topology by use of gene fusions and trypsin-sensitive insertions.

Authors:  M H Lee; N Kosuk; J Bailey; B Traxler; C Manoil
Journal:  J Bacteriol       Date:  1999-10       Impact factor: 3.490

Review 2.  Membrane topology and insertion of membrane proteins: search for topogenic signals.

Authors:  M van Geest; J S Lolkema
Journal:  Microbiol Mol Biol Rev       Date:  2000-03       Impact factor: 11.056

3.  Characterization of transmembrane segments 3, 4, and 5 of MalF by mutational analysis.

Authors:  A Steinke; S Grau; A Davidson; E Hofmann; M Ehrmann
Journal:  J Bacteriol       Date:  2001-01       Impact factor: 3.490

4.  Biotinylation in vivo as a sensitive indicator of protein secretion and membrane protein insertion.

Authors:  G Jander; J E Cronan; J Beckwith
Journal:  J Bacteriol       Date:  1996-06       Impact factor: 3.490

5.  Characterization of transmembrane domains 6, 7, and 8 of MalF by mutational analysis.

Authors:  R Ehrle; C Pick; R Ulrich; E Hofmann; M Ehrmann
Journal:  J Bacteriol       Date:  1996-04       Impact factor: 3.490

6.  Topology of the membrane protein LamB by epitope tagging and a comparison with the X-ray model.

Authors:  S M Newton; P E Klebba; V Michel; M Hofnung; A Charbit
Journal:  J Bacteriol       Date:  1996-06       Impact factor: 3.490

7.  Aeropyrum pernix membrane topology of protein VKOR promotes protein disulfide bond formation in two subcellular compartments.

Authors:  Stijntje Hibender; Cristina Landeta; Mehmet Berkmen; Jon Beckwith; Dana Boyd
Journal:  Microbiology       Date:  2017-11-15       Impact factor: 2.777

8.  Ferric citrate transport of Escherichia coli: functional regions of the FecR transmembrane regulatory protein.

Authors:  D Welz; V Braun
Journal:  J Bacteriol       Date:  1998-05       Impact factor: 3.490

9.  Cell cycle-controlled proteolysis of a flagellar motor protein that is asymmetrically distributed in the Caulobacter predivisional cell.

Authors:  U Jenal; L Shapiro
Journal:  EMBO J       Date:  1996-05-15       Impact factor: 11.598

10.  Interactions of VirB9, -10, and -11 with the membrane fraction of Agrobacterium tumefaciens: solubility studies provide evidence for tight associations.

Authors:  K E Finberg; T R Muth; S P Young; J B Maken; S M Heitritter; A N Binns; L M Banta
Journal:  J Bacteriol       Date:  1995-09       Impact factor: 3.490
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