Literature DB >> 3317413

Determinants of membrane protein topology.

D Boyd1, C Manoil, J Beckwith.   

Abstract

The topology of the integral membrane protein MalF, which is required for maltose transport in Escherichia coli, has been analyzed using fusions of alkaline phosphatase (EC 3.1.3.1). The properties of such fusion strains support a MalF structure previously proposed on theoretical grounds. Several transmembrane segments within MalF can act as signal sequences in exporting alkaline phosphatase. Other transmembrane sequences, in conjunction with cytoplasmic domains, can stably anchor alkaline phosphatase in the cytoplasm. Our results suggest that features of the amino acid sequence (possibly the positively charged amino acids) of the cytoplasmic domains of membrane proteins are important in anchoring these domains in the cytoplasm. These studies in conjunction with our earlier results show that alkaline phosphatase fusions to membrane proteins can be an important aid in analyzing membrane topology and its determinants.

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Year:  1987        PMID： 3317413      PMCID： PMC299577          DOI： 10.1073/pnas.84.23.8525

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  22 in total

1.  RELEASE OF ALKALINE PHOSPHATASE FROM CELLS OF ESCHERICHIA COLI UPON LYSOZYME SPHEROPLAST FORMATION.

Authors:  M H MALAMY; B L HORECKER
Journal:  Biochemistry       Date:  1964-12       Impact factor: 3.162

2.  The use of transposon TnphoA to detect genes for cell envelope proteins subject to a common regulatory stimulus. Analysis of osmotically regulated genes in Escherichia coli.

Authors:  C Gutierrez; J Barondess; C Manoil; J Beckwith
Journal:  J Mol Biol       Date:  1987-05-20       Impact factor: 5.469

3.  A plasmid cloning system utilizing replication and packaging functions of the filamentous bacteriophage fd.

Authors:  K Geider; C Hohmeyer; R Haas; T F Meyer
Journal:  Gene       Date:  1985       Impact factor: 3.688

4.  The human glucose transporter can insert posttranslationally into microsomes.

Authors:  M Mueckler; H F Lodish
Journal:  Cell       Date:  1986-02-28       Impact factor: 41.582

5.  The nucleotide sequence of the gene for malF protein, an inner membrane component of the maltose transport system of Escherichia coli. Repeated DNA sequences are found in the malE-malF intercistronic region.

Authors:  S Froshauer; J Beckwith
Journal:  J Biol Chem       Date:  1984-09-10       Impact factor: 5.157

6.  Cloning vectors that yield high levels of single-stranded DNA for rapid DNA sequencing.

Authors:  R J Zagursky; M L Berman
Journal:  Gene       Date:  1984-02       Impact factor: 3.688

7.  Interference resistant mutants of phage f1.

Authors:  V Enea; N D Zinder
Journal:  Virology       Date:  1982-10-15       Impact factor: 3.616

8.  Trypsin modification of Escherichia coli alkaline phosphatase.

Authors:  C H Roberts; J F Chlebowski
Journal:  J Biol Chem       Date:  1984-01-25       Impact factor: 5.157

9.  Mutations that alter the signal sequence of alkaline phosphatase in Escherichia coli.

Authors:  S Michaelis; H Inouye; D Oliver; J Beckwith
Journal:  J Bacteriol       Date:  1983-04       Impact factor: 3.490

10.  The distribution of positively charged residues in bacterial inner membrane proteins correlates with the trans-membrane topology.

Authors:  G Heijne
Journal:  EMBO J       Date:  1986-11       Impact factor: 11.598
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  130 in total

1.  Analysis of F factor TraD membrane topology by use of gene fusions and trypsin-sensitive insertions.

Authors:  M H Lee; N Kosuk; J Bailey; B Traxler; C Manoil
Journal:  J Bacteriol       Date:  1999-10       Impact factor: 3.490

2.  The ArcB sensor kinase of Escherichia coli: genetic exploration of the transmembrane region.

Authors:  O Kwon; D Georgellis; A S Lynch; D Boyd; E C Lin
Journal:  J Bacteriol       Date:  2000-05       Impact factor: 3.490

3.  Characterization of transmembrane segments 3, 4, and 5 of MalF by mutational analysis.

Authors:  A Steinke; S Grau; A Davidson; E Hofmann; M Ehrmann
Journal:  J Bacteriol       Date:  2001-01       Impact factor: 3.490

4.  Septation, dephosphorylation, and the activation of sigmaF during sporulation in Bacillus subtilis.

Authors:  N King; O Dreesen; P Stragier; K Pogliano; R Losick
Journal:  Genes Dev       Date:  1999-05-01       Impact factor: 11.361

5.  Membrane topology of the ZntB efflux system of Salmonella enterica serovar Typhimurium.

Authors:  Andreia M Caldwell; Ronald L Smith
Journal:  J Bacteriol       Date:  2003-01       Impact factor: 3.490

6.  A family of receptor-linked protein tyrosine phosphatases in humans and Drosophila.

Authors:  M Streuli; N X Krueger; A Y Tsai; H Saito
Journal:  Proc Natl Acad Sci U S A       Date:  1989-11       Impact factor: 11.205

7.  Topology of OxlT, the oxalate transporter of Oxalobacter formigenes, determined by site-directed fluorescence labeling.

Authors:  L Ye; Z Jia; T Jung; P C Maloney
Journal:  J Bacteriol       Date:  2001-04       Impact factor: 3.490

8.  Evidence that subcellular localization of a bacterial membrane protein is achieved by diffusion and capture.

Authors:  David Z Rudner; Qi Pan; Richard M Losick
Journal:  Proc Natl Acad Sci U S A       Date:  2002-06-11       Impact factor: 11.205

9.  The N-terminal domain of OmpATb is required for membrane translocation and pore-forming activity in mycobacteria.

Authors:  Anuradha Alahari; Nathalie Saint; Sylvie Campagna; Virginie Molle; Gérard Molle; Laurent Kremer
Journal:  J Bacteriol       Date:  2007-06-15       Impact factor: 3.490

10.  Membrane topology analysis of cyclic glucan synthase, a virulence determinant of Brucella abortus.

Authors:  Andrés E Ciocchini; Mara S Roset; Nora Iñón de Iannino; Rodolfo A Ugalde
Journal:  J Bacteriol       Date:  2004-11       Impact factor: 3.490
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